165,203 results

1. pHAGE2-TetOminiCMV-mCherry

   Plasmid

   #136615

   Purpose

   Dox-inducible lentivral vector expressing mCherry red fluorescent protein

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-O-FUW-FOSL2-P2A-mCherry

   Plasmid

   #203860

   Purpose

   Tet-inducible lentiviral plasmid expressing FOSL2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   FOSL2 (FOSL2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

   Plasmid

   #35511

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Halo-DNAPKcs HRD

   Plasmid

   #207088

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous DNA-PKcs locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag with internal PuroR cassette flanked by human DNAPKcs locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET FLIPPi-5u

   Plasmid

   #13554

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi S52A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   S52A in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rc/CMV cyclin C

   Plasmid

   #8961

   Depositor

   Bob Weinberg

   Article

   Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

   Insert

   cyclin C (CCNC Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pUdO1k

   Plasmid

   #210234

   Purpose

   high-copy plasmid for bacterial expression

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-p47-Strep-HA

   Plasmid

   #113475

   Purpose

   Expression of human p47 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   p47 (NSFL1C Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEAT8-137 M1V

   Plasmid

   #173807

   Purpose

   Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

   Depositor

   Anne Gershenson

   Article

   Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

   Insert

   SERPiNA1 (SERPINA1 Human)
   

   Expression

   Bacterial

   Mutation

   E1M Otherwise this plasmid encodes the most commo…

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 8522-M31-663

    Plasmid

    #225661

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCherry
    

    Use

    Lentiviral

    Promoter

    SORE6-mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IZ-HaloTag7-Galectin3

    Plasmid

    #184896

    Purpose

    Stable expression of HaloTag7-Galectin3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    Galectin3
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIG PIK3CA Wildtype

    Plasmid

    #73056

    Purpose

    Human PIK3CA Wildtype

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    PIK3CA Wildtype (PIK3CA Human)
    

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. huntingtin_FL_NbioCflag

    Plasmid

    #194767

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    Q23 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA-HA-TRAF3IP3 (mouse)

    Plasmid

    #71459

    Purpose

    Inducible expression of TRAF3IP3 in mammalian cells

    Depositor

    Shao-Cong Sun

    Article

    Zou et al J Exp Med. 2015 Jul 27;212(8):1323-36. d

    Insert

    TRAF3IP3 (Traf3ip3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

    Plasmid

    #50030

    Purpose

    Expression of cytosolic S. pyogenes SrtA in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

    Insert

    SrtA(strep)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-SAKS1-Strep-HA

    Plasmid

    #113487

    Purpose

    Expression of human SAKS1 with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    SAKS1 (UBXN1 Human)
    

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

    Plasmid

    #191391

    Purpose

    Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(30-120)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS0806 His14-bdSUMO-AwaIscB

    Plasmid

    #176539

    Purpose

    Bacterial expression of His14-bdSUMO-AwaIscB

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    AwaIscB
    

    Use

    CRISPR

    Tags

    14xHis-bdSUMO tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 Flag Fli1

    Plasmid

    #66976

    Purpose

    Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Fli1 (Fli1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. NEK7_HUMAN_D0

    Plasmid

    #79735

    Purpose

    This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK7 (NEK7 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only