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pcDNA3-EGFP-RhoA-T19N Citations (34)

Originally described in: Rho family proteins modulate rapid apoptosis induced by cytotoxic T lymphocytes and Fas.
Subauste MC, Von Herrath M, Benard V, Chamberlain CE, Chuang TH, Chu K, Bokoch GM, Hahn KM J Biol Chem. 2000 Mar 31. 275(13):9725-33.
PubMed Journal

Articles Citing pcDNA3-EGFP-RhoA-T19N

Geldanamycin anisimycins activate Rho and stimulate Rho- and ROCK-dependent actin stress fiber formation. Amiri A, Noei F, Feroz T, Lee JM. Mol Cancer Res. 2007 Sep;5(9):933-42. PubMed
Treponema denticola Msp-deduced peptide conjugate, P34BSA, promotes RhoA-dependent actin stress fiber formation independent of its internalization by fibroblasts. Amin M, Magnusson KE, Kapus A, Glogauer M, Ellen RP. Cell Motil Cytoskeleton. 2008 May;65(5):406-21. doi: 10.1002/cm.20270. PubMed
Structure based design of a Ca2+-sensitive RhoA protein that controls cell morphology. Mills E, Pham E, Truong K. Cell Calcium. 2010 Oct;48(4):195-201. PubMed

Associated Plasmids

Polarized migration of lymphatic endothelial cells is critically dependent on podoplanin regulation of Cdc42. Navarro A, Perez RE, Rezaiekhaligh MH, Mabry SM, Ekekezie II. Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L32-42. doi: 10.1152/ajplung.00171.2010. Epub 2010 Oct 29. PubMed
Mechanisms by which the inhibition of specific intracellular signaling pathways increase osteoblast proliferation on apatite surfaces. Yang S, Tian YS, Lee YJ, Yu FH, Kim HM. Biomaterials. 2011 Apr;32(11):2851-61. doi: 10.1016/j.biomaterials.2011.01.015. Epub 2011 Feb 1. PubMed
WT1-interacting protein (Wtip) regulates podocyte phenotype by cell-cell and cell-matrix contact reorganization. Kim JH, Mukherjee A, Madhavan SM, Konieczkowski M, Sedor JR. Am J Physiol Renal Physiol. 2012 Jan 1;302(1):F103-15. doi: 10.1152/ajprenal.00419.2011. Epub 2011 Sep 7. PubMed
The RhoA-ROCK-PTEN pathway as a molecular switch for anchorage dependent cell behavior. Yang S, Kim HM. Biomaterials. 2012 Apr;33(10):2902-15. doi: 10.1016/j.biomaterials.2011.12.051. Epub 2012 Jan 13. PubMed
RhoA mediates flow-induced endothelial sprouting in a 3-D tissue analogue of angiogenesis. Song JW, Daubriac J, Tse JM, Bazou D, Munn LL. Lab Chip. 2012 Dec 7;12(23):5000-6. doi: 10.1039/c2lc40389g. PubMed
Foxc2 induces Wnt4 and Bmp4 expression during muscle regeneration and osteogenesis. Gozo MC, Aspuria PJ, Cheon DJ, Walts AE, Berel D, Miura N, Karlan BY, Orsulic S. Cell Death Differ. 2013 Aug;20(8):1031-42. doi: 10.1038/cdd.2013.34. Epub 2013 May 3. PubMed
Matrix identity and tractional forces influence indirect cardiac reprogramming. Kong YP, Carrion B, Singh RK, Putnam AJ. Sci Rep. 2013 Dec 11;3:3474. doi: 10.1038/srep03474. PubMed

Associated Plasmids

ROCK inhibition activates MCF-7 cells. Yang S, Kim HM. PLoS One. 2014 Feb 11;9(2):e88489. doi: 10.1371/journal.pone.0088489. eCollection 2014. PubMed
The PDZ-binding domain of syndecan-2 inhibits LFA-1 high-affinity conformation. Rovira-Clave X, Angulo-Ibanez M, Reina M, Espel E. Cell Signal. 2014 Jul;26(7):1489-99. doi: 10.1016/j.cellsig.2014.03.012. Epub 2014 Mar 21. PubMed

Associated Plasmids

Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. Bentzinger CF, von Maltzahn J, Dumont NA, Stark DA, Wang YX, Nhan K, Frenette J, Cornelison DD, Rudnicki MA. J Cell Biol. 2014 Apr 14;205(1):97-111. doi: 10.1083/jcb.201310035. Epub 2014 Apr 7. PubMed
Hypertension Causing Mutations in Cullin3 Impair RhoA Ubiquitination and Augment Association with Substrate Adaptors. Ibeawuchi SC, Agbor LN, Quelle FW, Sigmund CD. J Biol Chem. 2015 Jun 22. pii: jbc.M115.645358. PubMed
Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization. Lomakin AJ, Lee KC, Han SJ, Bui DA, Davidson M, Mogilner A, Danuser G. Nat Cell Biol. 2015 Nov;17(11):1435-45. doi: 10.1038/ncb3246. Epub 2015 Sep 28. PubMed
Amphetamine activates Rho GTPase signaling to mediate dopamine transporter internalization and acute behavioral effects of amphetamine. Wheeler DS, Underhill SM, Stolz DB, Murdoch GH, Thiels E, Romero G, Amara SG. Proc Natl Acad Sci U S A. 2015 Dec 22;112(51):E7138-47. doi: 10.1073/pnas.1511670112. Epub 2015 Nov 9. PubMed
Caveolin-1-mediated Japanese encephalitis virus entry requires a two-step regulation of actin reorganization. Xu Q, Cao M, Song H, Chen S, Qian X, Zhao P, Ren H, Tang H, Wang Y, Wei Y, Zhu Y, Qi Z. Future Microbiol. 2016 Mar 17. PubMed
Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation. Yuan X, Cao J, He X, Serra R, Qu J, Cao X, Yang S. Nat Commun. 2016 Mar 21;7:11024. doi: 10.1038/ncomms11024. PubMed
The Andes Virus Nucleocapsid Protein Directs Basal Endothelial Cell Permeability by Activating RhoA. Gorbunova EE, Simons MJ, Gavrilovskaya IN, Mackow ER. MBio. 2016 Oct 25;7(5). pii: e01747-16. doi: 10.1128/mBio.01747-16. PubMed
Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. Rafiq NB, Lieu ZZ, Jiang T, Yu CH, Matsudaira P, Jones GE, Bershadsky AD. J Cell Biol. 2017 Jan 2;216(1):181-197. doi: 10.1083/jcb.201605104. Epub 2016 Dec 22. PubMed
Influence of Inherent Mechanophenotype on Competitive Cellular Adherence. Shah MK, Garcia-Pak IH, Darling EM. Ann Biomed Eng. 2017 Aug;45(8):2036-2047. doi: 10.1007/s10439-017-1841-5. Epub 2017 Apr 26. PubMed
A Cytokine-like Protein DKK3 Is Atheroprotective. Yu B, Kiechl S, Qi D, Wang X, Song Y, Weger S, Mayr A, Le Bras A, Karamariti E, Zhang Z, Del Barco Barrantes I, Niehrs C, Schett G, Hu Y, Wang W, Willeit J, Qu A, Xu Q. Circulation. 2017 Jul 3. pii: CIRCULATIONAHA.117.027690. doi: 10.1161/CIRCULATIONAHA.117.027690. PubMed
Dendritic remodeling of D1 neurons by RhoA/Rho-kinase mediates depression-like behavior. Fox ME, Chandra R, Menken MS, Larkin EJ, Nam H, Engeln M, Francis TC, Lobo MK. Mol Psychiatry. 2018 Aug 17. pii: 10.1038/s41380-018-0211-5. doi: 10.1038/s41380-018-0211-5. PubMed
PKCepsilon Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Schaffer TB, Smith JE, Cook EK, Phan T, Margolis SS. Cell Rep. 2018 Nov 27;25(9):2470-2483.e8. doi: 10.1016/j.celrep.2018.11.005. PubMed
Sterol regulatory element binding protein 1 couples mechanical cues and lipid metabolism. Bertolio R, Napoletano F, Mano M, Maurer-Stroh S, Fantuz M, Zannini A, Bicciato S, Sorrentino G, Del Sal G. Nat Commun. 2019 Mar 22;10(1):1326. doi: 10.1038/s41467-019-09152-7. PubMed
Vav2 lacks Ca(2+) entry-promoting scaffolding functions unique to Vav1 and inhibits T cell activation via Cdc42. Fray MA, Charpentier JC, Sylvain NR, Seminario MC, Bunnell SC. J Cell Sci. 2020 Mar 13;133(5). pii: jcs.238337. doi: 10.1242/jcs.238337. PubMed
Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors. Shen W, Kilander MBC, Bridi MS, Frei JA, Niescier RF, Huang S, Lin YC. J Neurosci Res. 2020 Jun;98(6):1213-1231. doi: 10.1002/jnr.24608. Epub 2020 Mar 4. PubMed
The Inhibition of Wnt Restrain KRAS(G12V)-Driven Metastasis in Non-Small-Cell Lung Cancer. Hung PS, Huang MH, Kuo YY, Yang JC. Cancers (Basel). 2020 Mar 31;12(4). pii: cancers12040837. doi: 10.3390/cancers12040837. PubMed
Tension heterogeneity directs form and fate to pattern the myocardial wall. Priya R, Allanki S, Gentile A, Mansingh S, Uribe V, Maischein HM, Stainier DYR. Nature. 2020 Dec;588(7836):130-134. doi: 10.1038/s41586-020-2946-9. Epub 2020 Nov 18. PubMed
Neuropathy-causing TRPV4 mutations disrupt TRPV4-RhoA interactions and impair neurite extension. McCray BA, Diehl E, Sullivan JM, Aisenberg WH, Zaccor NW, Lau AR, Rich DJ, Goretzki B, Hellmich UA, Lloyd TE, Sumner CJ. Nat Commun. 2021 Mar 4;12(1):1444. doi: 10.1038/s41467-021-21699-y. PubMed
Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells. Wu D, Harrison DL, Szasz T, Yeh CF, Shentu TP, Meliton A, Huang RT, Zhou Z, Mutlu GM, Huang J, Fang Y. Nat Metab. 2021 May;3(5):714-727. doi: 10.1038/s42255-021-00390-y. Epub 2021 May 24. PubMed
Stress fiber strain recognition by the LIM protein testin is cryptic and mediated by RhoA. Sala S, Oakes PW. Mol Biol Cell. 2021 Aug 19;32(18):1758-1771. doi: 10.1091/mbc.E21-03-0156. Epub 2021 May 26. PubMed
EphA2 signaling within integrin adhesions regulates fibrillar adhesion elongation and fibronectin deposition. Finney AC, Scott ML, Reeves KA, Wang D, Alfaidi M, Schwartz JC, Chitmon CM, Acosta CH, Murphy JM, Alexander JS, Pattillo CB, Lim ST, Orr AW. Matrix Biol. 2021 Sep;103-104:1-21. doi: 10.1016/j.matbio.2021.09.001. Epub 2021 Sep 17. PubMed
A cell-based GEF assay reveals new substrates for DENN domains and a role for DENND2B in primary ciliogenesis. Kumar R, Francis V, Kulasekaran G, Khan M, Armstrong GAB, McPherson PS. Sci Adv. 2022 Feb 25;8(8):eabk3088. doi: 10.1126/sciadv.abk3088. Epub 2022 Feb 23. PubMed

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