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pET26b_Nb.b201

Plasmid

#131404

Purpose

E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

Depositor

Andrew Kruse

Article

McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

Insert

Nanobody b201

Tags

6xHis-tag and pelB leader

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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OPRM1-DuET

Plasmid

#213361

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRM1 (OPRM1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

Plasmid

#48285

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6

Expression

Bacterial

Available Since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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hKCNQ1 pCDNA3.1(-)

Plasmid

#219953

Purpose

Expresses human KCNQ1 in mammalian cells

Depositor

Niels Decher

Article

Kiper et al Proc Natl Acad Sci U S A. 2024 Jun 18;12

Insert

KCNQ1 (KCNQ1 Human)

Expression

Mammalian

Mutation

contains silent sequence variations

Promoter

CMV

Available Since

Aug. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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TDH3pro-rtTA-tetO7pro-T7pol-pRS413

Plasmid

#239293

Purpose

Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 polymerase with NLS and P278L
reverse tetracycline transactivator (rtTA)

Expression

Yeast

Mutation

Contains M2-SE-G72P mutations, described in Addge…

Promoter

TDH3 and tetracycline-responsive element (TRE) an…

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

Plasmid

#117661

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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MCS-3_Endofin_LgBiT

Plasmid

#182574

Purpose

This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.

Depositor

Bradley McConnell

Article

Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1

Insert

zinc finger FYVE-type (ZFYVE16 Human)

Tags

LgBiT (Large BiT)

Expression

Mammalian

Mutation

FYVE domain Q739-K806

Promoter

Herpes simplex virus (HSV)

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Ntn1-Fc-His

Plasmid

#72104

Purpose

Expresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn1 (Ntn1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-N EBNALP

Plasmid

#37959

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNALP (EBNA-LP H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
pCherry.90 beta

Plasmid

#108223

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 beta

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

mCherry-Hsp90b (HSP90AB1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2

Plasmid

#42175

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

T7

Available Since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
mCherry-BAF

Plasmid

#172447

Purpose

Expression of nuclear protein BAF with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

BAF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFG7m34GW

Plasmid

#133747

Purpose

Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

Depositor

Niko Geldner

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Type

Empty backbone

Expression

Plant

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_N_STA5A

Plasmid

#167799

Purpose

Expresses STA5A in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

STA5A (STAT5A Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL-CAG-NR5A1-FLAG

Plasmid

#242229

Purpose

Expression of full-length human SF-1 using lentivirus

Depositor

Courtney Hodges

Article

Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057

Insert

NR5A1 (NR5A1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-3HA

Plasmid

#247593

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xHA

Use

Unspecified

Promoter

read through

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NES-FRCaMPi-P2A-mGreenLantern

Plasmid

#232841

Purpose

AAV transfer plasmid for CAG-mediated co-expression of FRCaMPi and mGreenLantern

Depositor

Kiryl Piatkevich

Article

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-FRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-N EBNA3A

Plasmid

#37956

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA3A (EBNA-3A H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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repressilator (pZS1-lTlrLLtCL)

Plasmid

#26489

Depositor

Michael Elowitz

Article

Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

Insert

LacI, tetR

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-N EBNA3B

Plasmid

#37957

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

EBNA3B (EBNA-3B/EBNA-3C H. Herpesvirus 4 (EBV))

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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