166,778 results

1. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXG260

   Plasmid

   #141282

   Purpose

   CMV:EGFP (as control for pXG237)

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Sept. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. nesprin TSmod 25

   Plasmid

   #125555

   Purpose

   Nesprin-2G force sensor with cerulean-F25-venus TSmodule

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   mini-nesprin-2G (Syne2 Mouse)
   

   Tags

   cerulean, venus

   Expression

   Mammalian

   Mutation

   TSmod changed from mTFP to cerulean, flageliform …

   Promoter

   CMV

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIP GFP-Stx17 WT

   Plasmid

   #45909

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

   Insert

   Syntaxin 17 (STX17 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Promoter

   CMV

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

   Plasmid

   #169912

   Purpose

   Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   dCjCas9
   

   Use

   CRISPR

   Tags

   10XGCN4, 2xSV40NLS, and SV40NLS-HA

   Expression

   Mammalian

   Mutation

   D8A, H559A in cjCas9

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

   Plasmid

   #223149

   Purpose

   Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-3xGFP-tDeg

   Plasmid

   #191371

   Purpose

   Overexpression of 3xGFP-tDeg in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   3xGFP-tDeg
   

   Tags

   3xGFP

   Expression

   Mammalian

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

   Plasmid

   #207880

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRDA_336

   Plasmid

   #179095

   Purpose

   BE Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   BE3.9 (Cas9-NG)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. #2.4 pre-G-SynapShot-TagBFP

    Plasmid

    #214721

    Purpose

    Expresses green #2.4 pre-SynapShot and TagBFP

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #2.4 pre-G-SynapShot
    

    Use

    AAV

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPAP004

    Plasmid

    #160911

    Purpose

    E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

    Insert

    lacZ selection cassette
    

    Expression

    Yeast

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHA2

    Plasmid

    #85710

    Purpose

    This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

    Depositor

    Ulrich Koszinowski, Martin Messerle

    Article

    Adler et al J Virol. 2000 Aug;74(15):6964-74.

    Type

    Empty backbone

    Use

    Cre/Lox; Herpesvirus cloning

    Expression

    Bacterial

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 TAP DHX36

    Plasmid

    #53547

    Purpose

    mammalian expression of DHX36

    Depositor

    Kathleen Collins

    Article

    Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    TAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet1_CoiA1_CoiB

    Plasmid

    #208761

    Purpose

    Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

    Depositor

    Wilfred van der Donk

    Article

    Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

    Inserts

    CoiA1
    CoiB
    

    Tags

    Hisx6 Tag and SUMO

    Expression

    Bacterial

    Mutation

    Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

    Promoter

    T7

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CD74-ROS1 nonmutant

    Plasmid

    #183813

    Purpose

    Express CD74-ROS1 fusion (C6;R34) in mammalian cells

    Depositor

    Aaron Hata

    Article

    Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

    Inserts

    ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
    CD74 molecule (CD74 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of CD74 exon 1-6 and ROS1 exon…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. #2.4 post-G-SynapShot-mScarlet

    Plasmid

    #214720

    Purpose

    Expresses green #2.4 post-SynapShot and mScarlet

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #2.4 post-G-SynapShot
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIB4

    Plasmid

    #25453

    Depositor

    Benjamin Glick

    Article

    Sears et al Yeast. 1998 Jun 15;14(8):783-90.

    Insert

    Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
    

    Use

    Pichia pastoris expression

    Available Since

    Feb. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-R-iLACCO1

    Plasmid

    #208025

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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