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Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA.
Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50716pCAG-EGxxFP5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.
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50717pCAG-EGxxFP-Cetn1Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.
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50718pX330-Cetn1/1pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.
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