pLL3.7
(Plasmid #11795)

• Purpose: (Empty Backbone) 3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.
• Depositing Lab: Luk Parijs
• Publication: Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.
• Citations: 160 Articles

Backbone

• Vector backbone: pLL3.7
• Backbone size (bp): 7650
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Promoter: mouse U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: U6 (5'-cagtgcaggggaaagaatagtagac-3')

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

For protocols regarding the use of pLL3.7, visit: http://web.mit.edu/jacks-lab/protocols/rnairesources.htm

Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.