Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

gRNA_AAVS1-T2 Citations (30)

Plasmid Article: RNA-Guided Human Genome Engineering via Cas9.
Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM Science. 2013 Jan 3.
PubMed Journal

Articles Citing gRNA_AAVS1-T2

High-precision robotic microcontact printing (R-muCP) utilizing a vision guided selectively compliant articulated robotic arm. McNulty JD, Klann T, Sha J, Salick M, Knight GT, Turng LS, Ashton RS. Lab Chip. 2014 Jun 7;14(11):1923-30. doi: 10.1039/c3lc51137e. Epub 2014 Apr 24. PubMed
Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. PubMed

Associated Plasmids

Adenoviral vector DNA for accurate genome editing with engineered nucleases. Holkers M, Maggio I, Henriques SF, Janssen JM, Cathomen T, Goncalves MA. Nat Methods. 2014 Oct;11(10):1051-7. doi: 10.1038/nmeth.3075. Epub 2014 Aug 24. PubMed
Improving the specificity and efficacy of CRISPR/CAS9 and gRNA through target specific DNA reporter. Zhang JH, Pandey M, Kahler JF, Loshakov A, Harris B, Dagur PK, Mo Y, Simonds WF. J Biotechnol. 2014 Sep 2. pii: S0168-1656(14)00838-4. doi: 10.1016/j.jbiotec.2014.08.033. PubMed

Associated Plasmids

Off-target assessment of CRISPR-Cas9 guiding RNAs in human iPS and mouse ES cells. Tan EP, Li Y, Velasco-Herrera Mdel C, Yusa K, Bradley A. Genesis. 2015 Feb;53(2):225-36. doi: 10.1002/dvg.22835. Epub 2014 Dec 10. PubMed
Efficient and allele-specific genome editing of disease loci in human iPSCs. Smith C, Abalde-Atristain L, He C, Brodsky BR, Braunstein EM, Chaudhari P, Jang YY, Cheng L, Ye Z. Mol Ther. 2015 Mar;23(3):570-7. doi: 10.1038/mt.2014.226. Epub 2014 Nov 24. PubMed
Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9. Chen Y, Cao J, Xiong M, Petersen AJ, Dong Y, Tao Y, Huang CT, Du Z, Zhang SC. Cell Stem Cell. 2015 Jul 1. pii: S1934-5909(15)00261-1. doi: 10.1016/j.stem.2015.06.001. PubMed

Associated Plasmids

Research of methods to detect genomic mutations induced by CRISPR/Cas systems. Wang K, Mei DY, Liu QN, Qiao XH, Ruan WM, Huang T, Cao GS. J Biotechnol. 2015 Nov 20;214:128-32. doi: 10.1016/j.jbiotec.2015.09.029. Epub 2015 Sep 28. PubMed
High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells. Steyer B, Carlson-Stevermer J, Angenent-Mari N, Khalil A, Harkness T, Saha K. Acta Biomater. 2015 Dec 30. pii: S1742-7061(15)30273-7. doi: 10.1016/j.actbio.2015.12.036. PubMed
Genome Editing in Human Pluripotent Stem Cells. Smith C, Ye Z, Cheng L. Cold Spring Harb Protoc. 2016 Apr 1;2016(4):pdb.top086819. doi: 10.1101/pdb.top086819. PubMed
Drug conjugated nanoparticles activated by cancer cell specific mRNA. Gossai NP, Naumann JA, Li NS, Zamora EA, Gordon DJ, Piccirilli JA, Gordon PM. Oncotarget. 2016 Jun 21;7(25):38243-38256. doi: 10.18632/oncotarget.9430. PubMed
RAC-tagging: Recombineering And Cas9-assisted targeting for protein tagging and conditional analyses. Baker O, Gupta A, Obst M, Zhang Y, Anastassiadis K, Fu J, Stewart AF. Sci Rep. 2016 May 24;6:25529. doi: 10.1038/srep25529. PubMed
Preparation and Analysis of Native Chromatin-Modifying Complexes. Doyon Y, Cote J. Methods Enzymol. 2016;573:303-18. doi: 10.1016/bs.mie.2016.01.017. Epub 2016 Mar 16. PubMed
Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing in Human Cells. Havlicek S, Shen Y, Alpagu Y, Bruntraeger MB, Zufir NB, Phuah ZY, Fu Z, Dunn NR, Stanton LW. Mol Ther. 2017 Feb 1;25(2):342-355. doi: 10.1016/j.ymthe.2016.11.007. PubMed

Associated Plasmids

A platform for functional assessment of large variant libraries in mammalian cells. Matreyek KA, Stephany JJ, Fowler DM. Nucleic Acids Res. 2017 Mar 15. doi: 10.1093/nar/gkx183. PubMed
Genome Editing in Human Pluripotent Stem Cells. Carlson-Stevermer J, Saha K. Methods Mol Biol. 2017;1590:165-174. doi: 10.1007/978-1-4939-6921-0_12. PubMed
An Organoid-Based Model of Cortical Development Identifies Non-Cell-Autonomous Defects in Wnt Signaling Contributing to Miller-Dieker Syndrome. Iefremova V, Manikakis G, Krefft O, Jabali A, Weynans K, Wilkens R, Marsoner F, Brandl B, Muller FJ, Koch P, Ladewig J. Cell Rep. 2017 Apr 4;19(1):50-59. doi: 10.1016/j.celrep.2017.03.047. PubMed
Marker-free coselection for CRISPR-driven genome editing in human cells. Agudelo D, Duringer A, Bozoyan L, Huard CC, Carter S, Loehr J, Synodinou D, Drouin M, Salsman J, Dellaire G, Laganiere J, Doyon Y. Nat Methods. 2017 Apr 17. doi: 10.1038/nmeth.4265. PubMed

Associated Plasmids

In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting. Chen X, Janssen JM, Liu J, Maggio I, 't Jong AEJ, Mikkers HMM, Goncalves MAFV. Nat Commun. 2017 Sep 22;8(1):657. doi: 10.1038/s41467-017-00687-1. PubMed
Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing. Carlson-Stevermer J, Abdeen AA, Kohlenberg L, Goedland M, Molugu K, Lou M, Saha K. Nat Commun. 2017 Nov 23;8(1):1711. doi: 10.1038/s41467-017-01875-9. PubMed
Efficiency and Specificity of Targeted Integration Mediated by the Adeno-Associated Virus Serotype 2 Rep 78 Protein. Li P, Marino MP, Zou J, Argaw T, Morreale MT, Iaffaldano BJ, Reiser J. Hum Gene Ther Methods. 2018 Jun;29(3):135-145. doi: 10.1089/hgtb.2018.052. PubMed
Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway. Nogimori T, Nishiura K, Kawashima S, Nagai T, Oishi Y, Hosoda N, Imataka H, Kitamura Y, Kitade Y, Hoshino SI. Nucleic Acids Res. 2019 Jan 10;47(1):432-449. doi: 10.1093/nar/gky1087. PubMed
A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing. Chen G, Abdeen AA, Wang Y, Shahi PK, Robertson S, Xie R, Suzuki M, Pattnaik BR, Saha K, Gong S. Nat Nanotechnol. 2019 Oct;14(10):974-980. doi: 10.1038/s41565-019-0539-2. Epub 2019 Sep 9. PubMed
LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Chung J, Wu X, Lambert TJ, Lai ZW, Walther TC, Farese RV Jr. Dev Cell. 2019 Dec 2;51(5):551-563.e7. doi: 10.1016/j.devcel.2019.10.006. Epub 2019 Nov 7. PubMed
Expanding the editable genome and CRISPR-Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking. Chen X, Tasca F, Wang Q, Liu J, Janssen JM, Brescia MD, Bellin M, Szuhai K, Kenrick J, Frock RL, Goncalves MAFV. Nucleic Acids Res. 2020 Jan 24;48(2):974-995. doi: 10.1093/nar/gkz1121. PubMed
Long-term development of human iPSC-derived pyramidal neurons quantified after transplantation into the neonatal mouse cortex. D'Alessio R, Koukouli F, Blanchard S, Catteau J, Rais C, Lemonnier T, Feraud O, Bennaceur-Griscelli A, Groszer M, Maskos U. Dev Biol. 2020 May 1;461(1):86-95. doi: 10.1016/j.ydbio.2020.01.009. Epub 2020 Jan 23. PubMed
MYCN amplification and ATRX mutations are incompatible in neuroblastoma. Zeineldin M, Federico S, Chen X, Fan Y, Xu B, Stewart E, Zhou X, Jeon J, Griffiths L, Nguyen R, Norrie J, Easton J, Mulder H, Yergeau D, Liu Y, Wu J, Van Ryn C, Naranjo A, Hogarty MD, Kaminski MM, Valentine M, Pruett-Miller SM, Pappo A, Zhang J, Clay MR, Bahrami A, Vogel P, Lee S, Shelat A, Sarthy JF, Meers MP, George RE, Mardis ER, Wilson RK, Henikoff S, Downing JR, Dyer MA. Nat Commun. 2020 Feb 14;11(1):913. doi: 10.1038/s41467-020-14682-6. PubMed
Global Landscape and Dynamics of Parkin and USP30-Dependent Ubiquitylomes in iNeurons during Mitophagic Signaling. Ordureau A, Paulo JA, Zhang J, An H, Swatek KN, Cannon JR, Wan Q, Komander D, Harper JW. Mol Cell. 2020 Mar 5;77(5):1124-1142.e10. doi: 10.1016/j.molcel.2019.11.013. PubMed
Fluorescent indicators for continuous and lineage-specific reporting of cell-cycle phases in human pluripotent stem cells. Chang Y, Hellwarth PB, Randolph LN, Sun Y, Xing Y, Zhu W, Lian XL, Bao X. Biotechnol Bioeng. 2020 Apr 11. doi: 10.1002/bit.27352. PubMed

Associated Plasmids

Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter. Butterfield KT, McGrath PS, Han CM, Kogut I, Bilousova G. Methods Mol Biol. 2020;2155:11-21. doi: 10.1007/978-1-0716-0655-1_2. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.