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pAdSh.U6.gRNAS1
(Plasmid #58252)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58252 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBR322-derived pAdEasy plasmid
  • Backbone size w/o insert (bp) 6930
  • Total vector size (bp) 7296
  • Vector type
    Mammalian Expression, Adenoviral, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    U6.gRNAS1 cassette
  • Species
    Synthetic
  • Insert Size (bp)
    366

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    RNA-guided human genome engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Feb 15;339(6121):823-6
  • Terms and Licenses

Depositor Comments


How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAdSh.U6.gRNAS1 was a gift from Manuel Goncalves (Addgene plasmid # 58252)
  • For your References section:

    Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells. Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA. Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105. 10.1038/srep05105 PubMed 24870050