Purpose(Empty Backbone) Adenoviral vector for the expression of the Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64072||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerGene Transfer Vector Core University of Iowa
- Backbone size (bp) 5679
- Promoter U6 and CBh
/ Fusion Protein
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Restriction Enzyme
- 5′ sequencing primer XhoI
- 3′ sequencing primer EcoRI (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byU6 and Cas9 from pX330 (Zhang Lab)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Adeno Cas9 was a gift from Andrea Ventura (Addgene plasmid # 64072 ; http://n2t.net/addgene:64072 ; RRID:Addgene_64072)
For your References section:In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han YC, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A. Nature. 2014 Dec 18;516(7531):423-7. doi: 10.1038/nature13902. Epub 2014 Oct 22. 10.1038/nature13902 PubMed 25337876