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Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells.
Maggio I, Holkers M, Liu J, Janssen JM, Chen X, Goncalves MA
Sci Rep. 2014 May 29;4:5105. doi: 10.1038/srep05105.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58252pAdSh.U6.gRNAS1U6 promoter-driven single guide RNA complementary to the human AAVS1 locus
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58253pAdSh.PGK.Cas9Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter
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58255pAdSh.U6.gRNAGFPU6 promoter-driven single guide RNA complementary to eGFP
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Antibodies from Article