XLone-Puro CCND2-T2A-luciferase-P2A-eGFP
(Plasmid
#179843)
-
PurposeTunable and temporal expression control of CCND2, luciferase and nuclear eGFP
-
Depositing Lab
-
Depositing OrganizationPurdue University
Located in United States of America -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179843 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 179843-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 179843-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneAddgene #140027
- Backbone size w/o insert (bp) 5760
- Total vector size (bp) 9146
-
Vector typeMammalian Expression, Luciferase, Synthetic Biology
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCCND2
-
Alt nameKIAK0002
-
Alt nameMPPH3
-
SpeciesH. sapiens (human)
-
Insert Size (bp)867
-
Entrez GeneCCND2 (a.k.a. KIAK0002, MPPH3)
- Promoter TRE3G
-
Tags
/ Fusion Proteins
- luciferase (C terminal on insert)
- eGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SpeI (not destroyed)
- 5′ sequencing primer None
- 3′ sequencing primer None
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 179843-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 179843-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
XLone-Puro CCND2-T2A-luciferase-P2A-eGFP was a gift from Xiaoping Bao (Addgene plasmid # 179843 ; http://n2t.net/addgene:179843 ; RRID:Addgene_179843) -
For your References section:
Optogenetic Control of Engrafted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Live Mice: A Proof-of-Concept Study. Joshi J, Xu B, Rubart M, Chang Y, Bao X, Chaliki HP, Scott LR, Zhu W. Cells. 2022 Mar 10;11(6). pii: cells11060951. doi: 10.3390/cells11060951. 10.3390/cells11060951 PubMed 35326403
Map uploaded by the depositor.
