PurposeExpresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||167983||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backboneCAG expression plasmid
- Backbone size w/o insert (bp) 5574
- Total vector size (bp) 11874
Vector typeMammalian Expression, CRISPR, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameTETv4 (TET1CD-XTEN80-dCas9)
SpeciesH. sapiens (human)
Insert Size (bp)6581
- Promoter CAG
/ Fusion Proteins
- TET1 catalytic domain (N terminal on insert)
- 3xNLS (C terminal on insert)
- tagBFP (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer ggcaaagaattctgcagtcg
- 3′ sequencing primer ccaccaccttctgataggc (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe dCas9 sequence was obtained from a previous CRISPRi construct (Gilbert et al., 2013). The TET1CD sequence was obtained from Fuw-dCas9-Tet1CD (Addgene #84475). XTEN linker sequences were previously published (Schellenberger et al., 2009).
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:TETv4 was a gift from Luke Gilbert (Addgene plasmid # 167983 ; http://n2t.net/addgene:167983 ; RRID:Addgene_167983)
For your References section:Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Nunez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS. Cell. 2021 Apr 7. pii: S0092-8674(21)00353-6. doi: 10.1016/j.cell.2021.03.025. 10.1016/j.cell.2021.03.025 PubMed 33838111