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Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonetopo
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 3952
- Total vector size (bp) 9370
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Vector typeMouse Targeting, Synthetic Biology
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Selectable markersPuromycin ; R166H in the PuroR. Puro selection has been confirmed to work well in mouse ES cells and fibroblasts.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
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SpeciesSynthetic
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAPDH-CPG-TV was a gift from Rudolf Jaenisch (Addgene plasmid # 70148 ; http://n2t.net/addgene:70148 ; RRID:Addgene_70148) -
For your References section:
Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046. 10.1016/j.cell.2015.08.046 PubMed 26406378
