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Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA
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Depositing Lab
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Depositing OrganizationWhitehead Institute for Biomedical Research
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 70148-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 70148-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonetopo
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 3952
- Total vector size (bp) 9370
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Vector typeMouse Targeting, Synthetic Biology
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Selectable markersPuromycin ; R166H in the PuroR. Puro selection has been confirmed to work well in mouse ES cells and fibroblasts.
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 70148-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 70148-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAPDH-CPG-TV was a gift from Rudolf Jaenisch (Addgene plasmid # 70148 ; http://n2t.net/addgene:70148 ; RRID:Addgene_70148) -
For your References section:
Tracing Dynamic Changes of DNA Methylation at Single-Cell Resolution. Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R. Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046. 10.1016/j.cell.2015.08.046 PubMed 26406378
