Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pcDNA3-EGFP-C4-Nrf2 Citations (27)

Originally described in: BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase.
Furukawa M, Xiong Y Mol Cell Biol. 2005 Jan . 25(1):162-71.
PubMed Journal

Articles Citing pcDNA3-EGFP-C4-Nrf2

Caveolin-1 inhibits expression of antioxidant enzymes through direct interaction with nuclear erythroid 2 p45-related factor-2 (Nrf2). Li W, Liu H, Zhou JS, Cao JF, Zhou XB, Choi AM, Chen ZH, Shen HH. J Biol Chem. 2012 Jun 15;287(25):20922-30. doi: 10.1074/jbc.M112.352336. Epub 2012 Apr 30. PubMed
The Keap1/Nrf2 protein axis plays a role in osteoclast differentiation by regulating intracellular reactive oxygen species signaling. Kanzaki H, Shinohara F, Kajiya M, Kodama T. J Biol Chem. 2013 Aug 9;288(32):23009-20. doi: 10.1074/jbc.M113.478545. Epub 2013 Jun 25. PubMed
Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes. Funakoshi-Hirose I, Aki T, Unuma K, Funakoshi T, Noritake K, Uemura K. Toxicology. 2013 Oct 4;312:74-82. doi: 10.1016/j.tox.2013.07.016. Epub 2013 Aug 7. PubMed
Methylated pentavalent arsenic metabolites are bifunctional inducers, as they induce cytochrome P450 1A1 and NAD(P)H:quinone oxidoreductase through AhR- and Nrf2-dependent mechanisms. Anwar-Mohamed A, Elshenawy OH, Soshilov AA, Denison MS, Chris Le X, Klotz LO, El-Kadi AO. Free Radic Biol Med. 2014 Feb;67:171-87. doi: 10.1016/j.freeradbiomed.2013.10.810. Epub 2013 Oct 24. PubMed
Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. Brzoska K, Stepkowski TM, Kruszewski M. Mol Cell Biochem. 2014 Apr;389(1-2):99-111. doi: 10.1007/s11010-013-1931-0. Epub 2014 Jan 5. PubMed
Lysophosphatidate signaling stabilizes Nrf2 and increases the expression of genes involved in drug resistance and oxidative stress responses: implications for cancer treatment. Venkatraman G, Benesch MG, Tang X, Dewald J, McMullen TP, Brindley DN. FASEB J. 2015 Mar;29(3):772-85. doi: 10.1096/fj.14-262659. Epub 2014 Nov 14. PubMed
CACUL1/CAC1 Regulates the Antioxidant Response by Stabilizing Nrf2. Kigoshi Y, Fukuda T, Endo T, Hayasaka N, Iemura S, Natsume T, Tsuruta F, Chiba T. Sci Rep. 2015 Aug 4;5:12857. doi: 10.1038/srep12857. PubMed
NRF2 Mediates Neuroblastoma Proliferation and Resistance to Retinoic Acid Cytotoxicity in a Model of In Vitro Neuronal Differentiation. de Miranda Ramos V, Zanotto-Filho A, de Bittencourt Pasquali MA, Klafke K, Gasparotto J, Dunkley P, Gelain DP, Moreira JC. Mol Neurobiol. 2015 Nov 5. PubMed
Alkylating Agent-Induced NRF2 Blocks Endoplasmic Reticulum Stress-Mediated Apoptosis via Control of Glutathione Pools and Protein Thiol Homeostasis. Zanotto-Filho A, Masamsetti VP, Loranc E, Tonapi SS, Gorthi A, Bernard X, Goncalves RM, Moreira JC, Chen Y, Bishop AJ. Mol Cancer Ther. 2016 Dec;15(12):3000-3014. doi: 10.1158/1535-7163.MCT-16-0271. Epub 2016 Sep 16. PubMed
T-REX on-demand redox targeting in live cells. Parvez S, Long MJ, Lin HY, Zhao Y, Haegele JA, Pham VN, Lee DK, Aye Y. Nat Protoc. 2016 Dec;11(12):2328-2356. doi: 10.1038/nprot.2016.114. Epub 2016 Oct 27. PubMed

Associated Plasmids

Nrf2 mutagenic activation drives hepatocarcinogenesis. Ngo HKC, Kim DH, Cha YN, Na HK, Surh YJ. Cancer Res. 2017 Jun 27. pii: canres.3538.2016. doi: 10.1158/0008-5472.CAN-16-3538. PubMed
Mitochondrial Complex I Reversible S-Nitrosation Improves Bioenergetics and Is Protective in Parkinson's Disease. Milanese C, Tapias V, Gabriels S, Cerri S, Levandis G, Blandini F, Tresini M, Shiva S, Greenamyre JT, Gladwin MT, Mastroberardino PG. Antioxid Redox Signal. 2018 Jan 1;28(1):44-61. doi: 10.1089/ars.2017.6992. Epub 2017 Sep 21. PubMed
Nrf2/ARE pathway attenuates oxidative and apoptotic response in human osteoarthritis chondrocytes by activating ERK1/2/ELK1-P70S6K-P90RSK signaling axis. Khan NM, Ahmad I, Haqqi TM. Free Radic Biol Med. 2018 Feb 20;116:159-171. doi: 10.1016/j.freeradbiomed.2018.01.013. Epub 2018 Jan 12. PubMed
Brain ischemic preconditioning protects against ischemic injury and preserves the blood-brain barrier via oxidative signaling and Nrf2 activation. Yang T, Sun Y, Mao L, Zhang M, Li Q, Zhang L, Shi Y, Leak RK, Chen J, Zhang F. Redox Biol. 2018 Jul;17:323-337. doi: 10.1016/j.redox.2018.05.001. Epub 2018 May 6. PubMed
Mild ozonisation activates antioxidant cell response by the Keap1/Nrf2 dependent pathway. Galie M, Costanzo M, Nodari A, Boschi F, Calderan L, Mannucci S, Covi V, Tabaracci G, Malatesta M. Free Radic Biol Med. 2018 Aug 20;124:114-121. doi: 10.1016/j.freeradbiomed.2018.05.093. Epub 2018 Jun 2. PubMed
Peroxidasin is a novel target of the redox-sensitive transcription factor Nrf2. Hanmer KL, Mavri-Damelin D. Gene. 2018 Oct 20;674:104-114. doi: 10.1016/j.gene.2018.06.076. Epub 2018 Jun 25. PubMed
TRIM16 controls assembly and degradation of protein aggregates by modulating the p62-NRF2 axis and autophagy. Jena KK, Kolapalli SP, Mehto S, Nath P, Das B, Sahoo PK, Ahad A, Syed GH, Raghav SK, Senapati S, Chauhan S, Chauhan S. EMBO J. 2018 Sep 14;37(18). pii: embj.201798358. doi: 10.15252/embj.201798358. Epub 2018 Aug 24. PubMed
Axonal pathology in hPSC-based models of Parkinson's disease results from loss of Nrf2 transcriptional activity at the Map1b gene locus. Czaniecki C, Ryan T, Stykel MG, Drolet J, Heide J, Hallam R, Wood S, Coackley C, Sherriff K, Bailey CDC, Ryan SD. Proc Natl Acad Sci U S A. 2019 Jul 9;116(28):14280-14289. doi: 10.1073/pnas.1900576116. Epub 2019 Jun 24. PubMed
Blockade of Glutathione Metabolism in IDH1-Mutated Glioma. Tang X, Fu X, Liu Y, Yu D, Cai SJ, Yang C. Mol Cancer Ther. 2020 Jan;19(1):221-230. doi: 10.1158/1535-7163.MCT-19-0103. Epub 2019 Sep 23. PubMed
AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes. Matzinger M, Fischhuber K, Poloske D, Mechtler K, Heiss EH. Redox Biol. 2020 Jan;29:101393. doi: 10.1016/j.redox.2019.101393. Epub 2019 Nov 27. PubMed
AMPK Enhances Transcription of Selected Nrf2 Target Genes via Negative Regulation of Bach1. Fischhuber K, Matzinger M, Heiss EH. Front Cell Dev Biol. 2020 Jul 14;8:628. doi: 10.3389/fcell.2020.00628. eCollection 2020. PubMed
MT1DP loaded by folate-modified liposomes sensitizes erastin-induced ferroptosis via regulating miR-365a-3p/NRF2 axis in non-small cell lung cancer cells. Gai C, Liu C, Wu X, Yu M, Zheng J, Zhang W, Lv S, Li W. Cell Death Dis. 2020 Sep 14;11(9):751. doi: 10.1038/s41419-020-02939-3. PubMed
Rosmarinic acid prevents refractory bacterial pneumonia through regulating Keap1/Nrf2-mediated autophagic pathway and mitochondrial oxidative stress. Zhang W, Cheng C, Sha Z, Chen C, Yu C, Lv N, Ji P, Wu X, Ma T, Cheng H, Shi L. Free Radic Biol Med. 2021 May 20;168:247-257. doi: 10.1016/j.freeradbiomed.2021.03.038. Epub 2021 Apr 1. PubMed
Upregulation of NOX-2 and Nrf-2 Promotes 5-Fluorouracil Resistance of Human Colon Carcinoma (HCT-116) Cells. Waghela BN, Vaidya FU, Pathak C. Biochemistry (Mosc). 2021 Mar;86(3):262-274. doi: 10.1134/S0006297921030044. PubMed
Small-molecule Akt-activation in airway cells induces NO production and reduces IL-8 transcription through Nrf-2. Gopallawa I, Kuek LE, Adappa ND, Palmer JN, Lee RJ. Respir Res. 2021 Oct 19;22(1):267. doi: 10.1186/s12931-021-01865-y. PubMed
PLK-1 Interacting Checkpoint Helicase, PICH, Mediates Cellular Oxidative Stress Response. Dutta A, Das A, Bisht D, Arya V, Muthuswami R. Epigenomes. 2022 Oct 18;6(4):36. doi: 10.3390/epigenomes6040036. PubMed
Protein Kinase B (PKB/AKT) Protects IDH-Mutated Glioma from Ferroptosis via Nrf2. Liu Y, Chou FJ, Lang F, Zhang M, Song H, Zhang W, Davis DL, Briceno NJ, Zhang Y, Cimino PJ, Zaghloul KA, Gilbert MR, Armstrong TS, Yang C. Clin Cancer Res. 2023 Apr 3;29(7):1305-1316. doi: 10.1158/1078-0432.CCR-22-3179. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.