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MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.
Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T
Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
63670pX330S-2-PITChExpresses Cas9 nuclease and the PITCh-gRNA
63671pX330A-FBL/PITChExpresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA
63672pCRIS-PITChv2-FBLPITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

Antibodies from Article