161,242 results

1. pCMV-MMLVgag-3xNES-ABE7.10-NG

   Plasmid

   #181753

   Purpose

   MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE7.10-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CbS-Flag-TRRAP

   Plasmid

   #32103

   Depositor

   Michael Cole, Yardena Samuels

   Insert

   Transformation/transcription domain-associated protein (TRRAP Human)
   

   Use

   Tags

   Flag 1x

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSmartBAC-S

   Plasmid

   #107268

   Purpose

   Broad host range bacterial artificial chromosome vector

   Depositor

   Ron Godiska, Mark Liles

   Article

   Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAW62.YY1.FKBP.knock-in.mCherry

   Plasmid

   #104370

   Purpose

   For knocking in FKBP F36V P2A mCherry onto YY1

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   FKBP12(F36V) -P2A-mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_PEmax-2A-MLH1dn-2A-BSD

   Plasmid

   #234072

   Purpose

   For lentiviral expression of PEmax with MLH1dn and blasticidin resistance

   Depositor

   Greg Findlay

   Article

   Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

   Insert

   PEmax-2A-MLH1dn
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α core promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. ABE8.20-NG

   Plasmid

   #226857

   Purpose

   Expresses ABE8.20-NG base editor in mammalian cells with eGFP marker

   Depositor

   Alexis Komor

   Article

   Osgood et al Methods in Enzymology, February 5, 2025

   Insert

   ABE8.20-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

   Plasmid

   #225753

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD-KIRIN1-GR

   Plasmid

   #107417

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1-GR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. iCas

   Plasmid

   #84232

   Purpose

   Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

   Depositor

   Meng How Tan

   Article

   Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   2A-OFP co-expression and ERT2-ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FUGW-eGFP-ZEB1 3'UTR

    Plasmid

    #81018

    Purpose

    FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    Zeb1 3'UTR (UTS2R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmScarlet3-Giantin_C1

    Plasmid

    #189773

    Purpose

    Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-Giantin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SETD2-GFP

    Plasmid

    #80653

    Purpose

    mammalian expression of SETD2-GFP

    Depositor

    Sérgio De Almeida

    Article

    Carvalho et al Elife. 2014 May 6;3:e02482. doi: 10.7554

    Insert

    SETD2 (SETD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScaf

    Plasmid

    #111401

    Purpose

    Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

    Plasmid

    #138148

    Purpose

    Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV mRuby3-Synapsin1a

    Plasmid

    #187896

    Purpose

    For labeling pre-synaptic terminals

    Depositor

    Timothy Ryan

    Insert

    Synapsin1a
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXJ-Nanoluc (Nluc)

    Plasmid

    #232656

    Purpose

    Nanoluc luciferase expression driven CMV promoter

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    Nanoluc
    

    Use

    Luciferase

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cys-3HB-SPYcatcher

    Plasmid

    #214838

    Purpose

    This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al bioRxiv 2024.01.21.576499.

    Insert

    Cys-3HB-SPYcatcher
    

    Use

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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