|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40822||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
- Promoter CMV
/ Fusion Protein
- EGFP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Please note that EcoRI is not a unique site. The alpha-synuclein insert contains an EcoRI site in the coding sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EGFP-alphasynuclein-WT was a gift from David Rubinsztein (Addgene plasmid # 40822 ; http://n2t.net/addgene:40822 ; RRID:Addgene_40822)
For your References section:Alpha-synuclein overexpression promotes aggregation of mutant huntingtin. Furlong RA, Narain Y, Rankin J, Wyttenbach A, Rubinsztein DC. Biochem J. 2000 Mar 15;346 Pt 3:577-81. 10.1042/0264-6021:3460577 PubMed 10698681