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pU6-sgRNA EF1Alpha-puro-T2A-BFP Citations (23)

Plasmid Article: Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. Epub 2014 Oct 9.
PubMed Journal

Articles Citing pU6-sgRNA EF1Alpha-puro-T2A-BFP

Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression. Radzisheuskaya A, Shlyueva D, Muller I, Helin K. Nucleic Acids Res. 2016 Oct 14;44(18):e141. Epub 2016 Jun 28. PubMed
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS. Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed

Associated Plasmids

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response. Adamson B, Norman TM, Jost M, Cho MY, Nunez JK, Chen Y, Villalta JE, Gilbert LA, Horlbeck MA, Hein MY, Pak RA, Gray AN, Gross CA, Dixit A, Parnas O, Regev A, Weissman JS. Cell. 2016 Dec 15;167(7):1867-1882.e21. doi: 10.1016/j.cell.2016.11.048. PubMed

Associated Plasmids

MELK is not necessary for the proliferation of basal-like breast cancer cells. Huang HT, Seo HS, Zhang T, Wang Y, Jiang B, Li Q, Buckley DL, Nabet B, Roberts JM, Paulk J, Dastjerdi S, Winter GE, McLauchlan H, Moran J, Bradner JE, Eck MJ, Dhe-Paganon S, Zhao JJ, Gray NS. Elife. 2017 Sep 19;6. pii: e26693. doi: 10.7554/eLife.26693. PubMed

Associated Plasmids

Distinct roles for phosphoinositide 3-kinases gamma and delta in malignant B cell migration. Ali AY, Wu X, Eissa N, Hou S, Ghia JE, Murooka TT, Banerji V, Johnston JB, Lin F, Gibson SB, Marshall AJ. Leukemia. 2018 Sep;32(9):1958-1969. doi: 10.1038/s41375-018-0012-5. Epub 2018 Jan 31. PubMed
P53 and mTOR signalling determine fitness selection through cell competition during early mouse embryonic development. Bowling S, Di Gregorio A, Sancho M, Pozzi S, Aarts M, Signore M, D Schneider M, Martinez-Barbera JP, Gil J, Rodriguez TA. Nat Commun. 2018 May 2;9(1):1763. doi: 10.1038/s41467-018-04167-y. PubMed
The ER membrane protein complex interacts cotranslationally to enable biogenesis of multipass membrane proteins. Shurtleff MJ, Itzhak DN, Hussmann JA, Schirle Oakdale NT, Costa EA, Jonikas M, Weibezahn J, Popova KD, Jan CH, Sinitcyn P, Vembar SS, Hernandez H, Cox J, Burlingame AL, Brodsky JL, Frost A, Borner GH, Weissman JS. Elife. 2018 May 29;7. pii: 37018. doi: 10.7554/eLife.37018. PubMed
Specificity of RNAi, LNA and CRISPRi as loss-of-function methods in transcriptional analysis. Stojic L, Lun ATL, Mangei J, Mascalchi P, Quarantotti V, Barr AR, Bakal C, Marioni JC, Gergely F, Odom DT. Nucleic Acids Res. 2018 Jul 6;46(12):5950-5966. doi: 10.1093/nar/gky437. PubMed
A Network of Noncoding Regulatory RNAs Acts in the Mammalian Brain. Kleaveland B, Shi CY, Stefano J, Bartel DP. Cell. 2018 Jul 12;174(2):350-362.e17. doi: 10.1016/j.cell.2018.05.022. Epub 2018 Jun 7. PubMed

Associated Plasmids

Evidence for an Integrated Gene Repression Mechanism Based on mRNA Isoform Toggling in Human Cells. Hollerer I, Barker JC, Jorgensen V, Tresenrider A, Dugast-Darzacq C, Chan LY, Darzacq X, Tjian R, Unal E, Brar GA. G3 (Bethesda). 2019 Apr 9;9(4):1045-1053. doi: 10.1534/g3.118.200802. PubMed
Ceapins block the unfolded protein response sensor ATF6alpha by inducing a neomorphic inter-organelle tether. Torres SE, Gallagher CM, Plate L, Gupta M, Liem CR, Guo X, Tian R, Stroud RM, Kampmann M, Weissman JS, Walter P. Elife. 2019 May 31;8. pii: 46595. doi: 10.7554/eLife.46595. PubMed
Integrated analyses of murine breast cancer models reveal critical parallels with human disease. Rennhack JP, To B, Swiatnicki M, Dulak C, Ogrodzinski MP, Zhang Y, Li C, Bylett E, Ross C, Szczepanek K, Hanrahan W, Jayatissa M, Lunt SY, Hunter K, Andrechek ER. Nat Commun. 2019 Jul 22;10(1):3261. doi: 10.1038/s41467-019-11236-3. PubMed
PUMILIO, but not RBMX, binding is required for regulation of genomic stability by noncoding RNA NORAD. Elguindy MM, Kopp F, Goodarzi M, Rehfeld F, Thomas A, Chang TC, Mendell JT. Elife. 2019 Jul 25;8. pii: 48625. doi: 10.7554/eLife.48625. PubMed
Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes. Temoche-Diaz MM, Shurtleff MJ, Nottingham RM, Yao J, Fadadu RP, Lambowitz AM, Schekman R. Elife. 2019 Aug 22;8. pii: 47544. doi: 10.7554/eLife.47544. PubMed
New factors for protein transport identified by a genome-wide CRISPRi screen in mammalian cells. Bassaganyas L, Popa SJ, Horlbeck M, Puri C, Stewart SE, Campelo F, Ashok A, Butnaru CM, Brouwers N, Heydari K, Ripoche J, Weissman J, Rubinsztein DC, Schekman R, Malhotra V, Moreau K, Villeneuve J. J Cell Biol. 2019 Nov 4;218(11):3861-3879. doi: 10.1083/jcb.201902028. Epub 2019 Sep 5. PubMed
PRMT5 methylome profiling uncovers a direct link to splicing regulation in acute myeloid leukemia. Radzisheuskaya A, Shliaha PV, Grinev V, Lorenzini E, Kovalchuk S, Shlyueva D, Gorshkov V, Hendrickson RC, Jensen ON, Helin K. Nat Struct Mol Biol. 2019 Nov;26(11):999-1012. doi: 10.1038/s41594-019-0313-z. Epub 2019 Oct 14. PubMed
A curative combination cancer therapy achieves high fractional cell killing through low cross-resistance and drug additivity. Palmer AC, Chidley C, Sorger PK. Elife. 2019 Nov 19;8. pii: 50036. doi: 10.7554/eLife.50036. PubMed
A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division. Stojic L, Lun ATL, Mascalchi P, Ernst C, Redmond AM, Mangei J, Barr AR, Bousgouni V, Bakal C, Marioni JC, Odom DT, Gergely F. Nat Commun. 2020 Apr 15;11(1):1851. doi: 10.1038/s41467-020-14978-7. PubMed
MicroRNA Clustering Assists Processing of Suboptimal MicroRNA Hairpins through the Action of the ERH Protein. Fang W, Bartel DP. Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 10.1016/j.molcel.2020.01.026. PubMed

Associated Plasmids

TETs compete with DNMT3 activity in pluripotent cells at thousands of methylated somatic enhancers. Charlton J, Jung EJ, Mattei AL, Bailly N, Liao J, Martin EJ, Giesselmann P, Brandl B, Stamenova EK, Muller FJ, Kiskinis E, Gnirke A, Smith ZD, Meissner A. Nat Genet. 2020 Aug;52(8):819-827. doi: 10.1038/s41588-020-0639-9. Epub 2020 Jun 8. PubMed
FOXO1 promotes HIV latency by suppressing ER stress in T cells. Vallejo-Gracia A, Chen IP, Perrone R, Besnard E, Boehm D, Battivelli E, Tezil T, Krey K, Raymond KA, Hull PA, Walter M, Habrylo I, Cruz A, Deeks S, Pillai S, Verdin E, Ott M. Nat Microbiol. 2020 Sep;5(9):1144-1157. doi: 10.1038/s41564-020-0742-9. Epub 2020 Jun 15. PubMed
Tuning the Expression of Long Noncoding RNA Loci with CRISPR Interference. Stojic L. Methods Mol Biol. 2020;2161:1-16. doi: 10.1007/978-1-0716-0680-3_1. PubMed
A Simple and Efficient Method to Generate Gene-Knockout and Transgenic Cell Lines. Liu J, Ge Y, Wang N, Wen J, Wang W, Zeng B, Chen GL. DNA Cell Biol. 2021 Feb;40(2):239-246. doi: 10.1089/dna.2020.6183. Epub 2020 Dec 31. PubMed

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