161,186 results

1. pJJB1578

   Plasmid

   #218620

   Purpose

   Express Pex22, Pex6, Pex15, and Pex8

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex22
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE pGK LysoFlux (HaloTag7-mGFP-Galectin3)

   Plasmid

   #215009

   Purpose

   Express a Lysophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

   Plasmid

   #215011

   Purpose

   Express a Mitophagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   pSu9 (Presequence of Fo-ATPase subunit 9)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

   Plasmid

   #215012

   Purpose

   Express a ERphagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   KDEL
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHAGE pGK ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

   Plasmid

   #215013

   Purpose

   Express a ERphagy reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   KDEL
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

   Plasmid

   #221385

   Purpose

   Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

   Depositor

   Junwei Shi

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2-3X-FLAG-H3.1-K27M-BFP

   Plasmid

   #209439

   Purpose

   SB-transposable plasmid expressing a FLAGGED mutant histone H3.1-K27M with BFP

   Depositor

   Maria Castro

   Insert

   H3.1-K27M
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K27M

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2-H3.1-WT-BFP

   Plasmid

   #209437

   Purpose

   SB-transposable plasmid expressing FLAGGED human histone mutant H3.1-Wt with BFP

   Depositor

   Maria Castro

   Insert

   H3.1-WT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-UbiC-GFP-ps

   Plasmid

   #183712

   Purpose

   GFP with palmitoylation signal

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Insert

   GFP with palmitoylation signal
   

   Use

   Lentiviral

   Tags

   palmitoylation signal

   Expression

   Mutation

   Promoter

   UbiC

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE7

    Plasmid

    #214812

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-GFP (PX458)

    Plasmid

    #48138

    Purpose

    Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-pegRNA-GG-acceptor

    Plasmid

    #132777

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    Exchangeable cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCL-Eco

    Plasmid

    #12371

    Depositor

    Inder Verma

    Article

    Naviaux et al J Virol. 1996 Aug . 70(8):5701-5.

    Insert

    gag/pol/env
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSIV-D3psi/delta env/delta Vif/delta Vpr

    Plasmid

    #132928

    Purpose

    Production of SIV-VLPs containing Vpx protein

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    Gag, pol, and vpx
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-evoCAST-eCas8

    Plasmid

    #234727

    Purpose

    Engineered human-codon optimized PseCas8 in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    ePseCas8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-evoCAST-evoTnsAB

    Plasmid

    #234729

    Purpose

    Evolved human-codon optimized PseTnsAB in mammalian cells (for evoCAST)

    Depositor

    David Liu

    Article

    Witte et al Science. 2025 May 15;388(6748):eadt5199.

    Insert

    evoPseTnsAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. psPAX2-D64V

    Plasmid

    #63586

    Purpose

    psPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.

    Depositor

    David Rawlings, Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Packaging plasmid for integrase deficient lentiviral vectors
    

    Use

    Integrase deficient lentiviral packaging vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-tevopreq1-GG-acceptor

    Plasmid

    #174038

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-FLAG-SF2

    Plasmid

    #99021

    Purpose

    Mammalian expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge