165,187 results

1. mEmerald-Rab7a-7

   Plasmid

   #54244

   Purpose

   Localization: Ras GTPase, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Rab7a (RAB7A Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCLASP_mScarlet_CLASP_RGS2membrane

   Plasmid

   #133086

   Purpose

   Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   mScarlet-CLASP
   

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCm C-term MBP NikJC199U

   Plasmid

   #174365

   Purpose

   Expression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.

   Depositor

   Brandon Greene

   Article

   Caceres et al Methods Enzymol. 2022;662:119-141. doi:

   Insert

   nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
   

   Tags

   8xHis, MBP, and TEV

   Expression

   Bacterial

   Mutation

   C199U

   Promoter

   T7

   Available Since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET19b-EcMscL-TEV-mEGFP

   Plasmid

   #165097

   Purpose

   Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

   Depositor

   Neha Kamat

   Article

   Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

   Insert

   E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
   

   Use

   Synthetic Biology

   Tags

   monomeric Enhanced Green Fluorescent Protein

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMR506-EF1a-H2B-EGFP

   Plasmid

   #186627

   Purpose

   Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

   Depositor

   Jonas Frisén

   Article

   Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

   Insert

   EF1a (EEF1A1 Synthetic)
   

   Use

   Lentiviral

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDF-mTET1

   Plasmid

   #81052

   Purpose

   bacterial expression of murine TET1

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TET1 (Tet1 Mouse)
   

   Tags

   6HIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMK1221

   Plasmid

   #84220

   Purpose

   Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry

   Depositor

   Martin Kampmann, Jonathan Weissman

   Article

   Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

   Plasmid

   #202822

   Purpose

   Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   two sgRNAs that delete exon 1 within the ZNF91 gene
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6 - two

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_TOMM20-STf

   Plasmid

   #182002

   Purpose

   CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   TOMM20-STf
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ρ1-EM GABAA receptor in pEZT-BM

    Plasmid

    #213072

    Purpose

    Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD

    Depositor

    Erik Lindahl

    Article

    Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.

    Insert

    Human GABAa rho1 receptor (GABRR1 Human)
    

    Use

    Bacmam, baculovirus, oocyte expression

    Tags

    Twin-Strep tag and superfolderGFP

    Expression

    Mammalian

    Mutation

    Encodes residues S58–L383 and D451–S479, separate…

    Promoter

    CMV and T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Annexin A7-TEV-TwinStrep

    Plasmid

    #198635

    Purpose

    Annexin A7 fibrillization and lipid binding

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 (ANXA7 Human)
    

    Tags

    TEV-GS-Dual Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. v5 ABE-eVLP

    Plasmid

    #228467

    Purpose

    Expresses MMLVgag(C507V)–ABE8e for producing v5 ABE-eVLPs

    Depositor

    David Liu

    Article

    Raguram et al Nat Biotechnol. 2024 Nov 13. doi: 10.103

    Insert

    MMLVgag(C507V)–ABE8e
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    MMLVgag(C507V)

    Promoter

    CMV

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid

    #58993

    Purpose

    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter

    Depositor

    Snorri Thorgeirsson

    Article

    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

    Inserts

    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    EcoRV site in MCS converted to BamHI site

    Promoter

    CMV enhancer-chicken beta actin

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HA

    Plasmid

    #128034

    Purpose

    Mammalian expression vector with N terminal HA tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. eeBxb1

    Plasmid

    #222339

    Purpose

    Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved and engineered Bxb1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW-Cas9

    Plasmid

    #50661

    Purpose

    Doxycycline-inducible lentiviral expression of SpCas9

    Depositor

    Eric Lander, David Sabatini

    Article

    Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    Tet ON

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET29b-10xHis-Super TEV

    Plasmid

    #193833

    Purpose

    Expresses high-efficiency TEV protease in bacterial cells

    Depositor

    EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

    Article

    Lau et al protocols.io, doi: 10.17504/protocols.io

    Insert

    TEV protease
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-TurboID

    Plasmid

    #124646

    Purpose

    Expresses Flag-TurboID fusion for proximity labeling

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-TurboCas-NLS-Blast

    Plasmid

    #232346

    Purpose

    Lentiviral expression vector for dCas9 fused to miniTurboNLS

    Depositor

    Ali Shilatifard

    Article

    Cenik et al Mol Cell. 2024 Dec 19;84(24):4929-4944.e

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA, miniTurbo, V5 and 3xSV40 NLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-CMV-jGCaMP8s

    Plasmid

    #162371

    Purpose

    Mammalian expression of ultrafast protein calcium sensor

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    CMV

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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