sortase A pentamutant (eSrtA) in pET29 Citations (25)
Originally described in: A general strategy for the evolution of bond-forming enzymes using yeast display.Chen I, Dorr BM, Liu DR Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404. doi: 10.1073/pnas.1101046108. Epub 2011 Jun 22. PubMed Journal
Articles Citing sortase A pentamutant (eSrtA) in pET29
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Covalently circularized nanodiscs for studying membrane proteins and viral entry.
Nasr ML, Baptista D, Strauss M, Sun ZJ, Grigoriu S, Huser S, Pluckthun A, Hagn F, Walz T, Hogle JM, Wagner G.
Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 2016 Nov 21.
PubMed
Associated Plasmids |
| Sortase A as a cross-linking enzyme in tissue engineering. Broguiere N, Formica FA, Barreto G, Zenobi-Wong M. Acta Biomater. 2018 Sep 1;77:182-190. doi: 10.1016/j.actbio.2018.07.020. Epub 2018 Jul 10. PubMed |
| Assembly and Translocation of a CRISPR-Cas Primed Acquisition Complex. Dillard KE, Brown MW, Johnson NV, Xiao Y, Dolan A, Hernandez E, Dahlhauser SD, Kim Y, Myler LR, Anslyn EV, Ke A, Finkelstein IJ. Cell. 2018 Nov 1;175(4):934-946.e15. doi: 10.1016/j.cell.2018.09.039. Epub 2018 Oct 18. PubMed |
| A Flow-Extension Tethered Particle Motion Assay for Single-Molecule Proteolysis. Drabek AA, Loparo JJ, Blacklow SC. Biochemistry. 2019 May 21;58(20):2509-2518. doi: 10.1021/acs.biochem.9b00106. Epub 2019 Apr 12. PubMed |
| Novel Method for Preparation of Site-Specific, Stoichiometric-Controlled Dual Warhead Conjugate of FGF2 via Dimerization Employing Sortase A-Mediated Ligation. Krzyscik MA, Opalinski L, Otlewski J. Mol Pharm. 2019 Aug 5;16(8):3588-3599. doi: 10.1021/acs.molpharmaceut.9b00434. Epub 2019 Jun 24. PubMed |
| Conformational Sensors and Domain Swapping Reveal Structural and Functional Differences between beta-Arrestin Isoforms. Ghosh E, Dwivedi H, Baidya M, Srivastava A, Kumari P, Stepniewski T, Kim HR, Lee MH, van Gastel J, Chaturvedi M, Roy D, Pandey S, Maharana J, Guixa-Gonzalez R, Luttrell LM, Chung KY, Dutta S, Selent J, Shukla AK. Cell Rep. 2019 Sep 24;28(13):3287-3299.e6. doi: 10.1016/j.celrep.2019.08.053. PubMed |
| Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging. Hebbrecht T, Liu J, Zwaenepoel O, Boddin G, Van Leene C, Decoene K, Madder A, Braeckmans K, Gettemans J. N Biotechnol. 2020 Nov 25;59:33-43. doi: 10.1016/j.nbt.2020.05.004. Epub 2020 Jul 10. PubMed |
| Site-Specific Biofunctionalization of Cellulose and Poly(dimethylsiloxane): A Chemoenzymatic Approach for Surface Engineering. Ibrar M, Zhang H. Langmuir. 2020 Dec 15;36(49):15039-15047. doi: 10.1021/acs.langmuir.0c02575. Epub 2020 Dec 4. PubMed |
| Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation. Tibble RW, Depaix A, Kowalska J, Jemielity J, Gross JD. Nat Chem Biol. 2021 May;17(5):615-623. doi: 10.1038/s41589-021-00774-x. Epub 2021 Mar 25. PubMed |
| A modular toolbox to generate complex polymeric ubiquitin architectures using orthogonal sortase enzymes. Fottner M, Weyh M, Gaussmann S, Schwarz D, Sattler M, Lang K. Nat Commun. 2021 Nov 11;12(1):6515. doi: 10.1038/s41467-021-26812-9. PubMed |
| Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4(+) Cells. Traenkle B, Kaiser PD, Pezzana S, Richardson J, Gramlich M, Wagner TR, Seyfried D, Weldle M, Holz S, Parfyonova Y, Nueske S, Scholz AM, Zeck A, Jakobi M, Schneiderhan-Marra N, Schaller M, Maurer A, Gouttefangeas C, Kneilling M, Pichler BJ, Sonanini D, Rothbauer U. Front Immunol. 2021 Dec 9;12:799910. doi: 10.3389/fimmu.2021.799910. eCollection 2021. PubMed |
| Covalent coupling of Spike's receptor binding domain to a multimeric carrier produces a high immune response against SARS-CoV-2. Sci Rep. 2022 Jan 13;12(1):692. doi: 10.1038/s41598-021-03675-0. PubMed |
| A Nanobody-Based Toolset to Monitor and Modify the Mitochondrial GTPase Miro1. Fagbadebo FO, Kaiser PD, Zittlau K, Bartlick N, Wagner TR, Froehlich T, Jarjour G, Nueske S, Scholz A, Traenkle B, Macek B, Rothbauer U. Front Mol Biosci. 2022 Mar 10;9:835302. doi: 10.3389/fmolb.2022.835302. eCollection 2022. PubMed |
| Delineating the mechanism of anti-Lassa virus GPC-A neutralizing antibodies. Enriquez AS, Buck TK, Li H, Norris MJ, Moon-Walker A, Zandonatti MA, Harkins SS, Robinson JE, Branco LM, Garry RF, Saphire EO, Hastie KM. Cell Rep. 2022 May 24;39(8):110841. doi: 10.1016/j.celrep.2022.110841. PubMed |
| A cocktail of protective antibodies subverts the dense glycan shield of Lassa virus. Li H, Buck T, Zandonatti M, Yin J, Moon-Walker A, Fang J, Koval A, Heinrich ML, Rowland MM, Diaz Avalos R, Schendel SL, Parekh D, Zyla D, Enriquez A, Harkins S, Sullivan B, Smith V, Chukwudozie O, Watanabe R, Robinson JE, Garry RF, Branco LM, Hastie KM, Saphire EO. Sci Transl Med. 2022 Oct 26;14(668):eabq0991. doi: 10.1126/scitranslmed.abq0991. Epub 2022 Oct 26. PubMed |
| Disordered Domain Shifts the Conformational Ensemble of the Folded Regulatory Domain of the Multidomain Oncoprotein c-Src. Gurumoorthy V, Shrestha UR, Zhang Q, Pingali SV, Boder ET, Urban VS, Smith JC, Petridis L, O'Neill H. Biomacromolecules. 2023 Feb 13;24(2):714-723. doi: 10.1021/acs.biomac.2c01158. Epub 2023 Jan 24. PubMed |
| User-Controlled 4D Biomaterial Degradation with Substrate-Selective Sortase Transpeptidases for Single-Cell Biology. Bretherton RC, Haack AJ, Kopyeva I, Rahman F, Kern JD, Bugg D, Theberge AB, Davis J, DeForest CA. Adv Mater. 2023 May;35(19):e2209904. doi: 10.1002/adma.202209904. Epub 2023 Mar 29. PubMed |
| Sortase A transpeptidation produces seamless, unbranched biotinylated nanobodies for multivalent and multifunctional applications. Obeng EM, Steer DL, Fulcher AJ, Wagstaff KM. Nanoscale Adv. 2023 Mar 15;5(8):2251-2260. doi: 10.1039/d3na00014a. eCollection 2023 Apr 11. PubMed |
| Rapid nanobody-based imaging of mesothelin expressing malignancies compatible with blocking therapeutic antibodies. Benloucif A, Meyer D, Balasse L, Goubard A, Danner L, Bouhlel A, Castellano R, Guillet B, Chames P, Kerfelec B. Front Immunol. 2023 Jun 14;14:1200652. doi: 10.3389/fimmu.2023.1200652. eCollection 2023. PubMed |
Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3.
Singh AK, Krezel A.
Microb Cell Fact. 2023 Jul 11;22(1):125. doi: 10.1186/s12934-023-02134-x.
PubMed
Associated Plasmids |
| Steric-Deficient Oligoglycine Surrogates Facilitate Multivalent and Bifunctional Nanobody Synthesis via Combined Sortase A Transpeptidation and Click Chemistry. Obeng EM, Steer DL, Fulcher A, Wagstaff KM. Bioconjug Chem. 2023 Sep 20;34(9):1667-1678. doi: 10.1021/acs.bioconjchem.3c00319. Epub 2023 Aug 3. PubMed |
| The intrinsically disordered cytoplasmic tail of a dendrite branching receptor uses two distinct mechanisms to regulate the actin cytoskeleton. Kramer DA, Narvaez-Ortiz HY, Patel U, Shi R, Shen K, Nolen BJ, Roche J, Chen B. Elife. 2023 Aug 9;12:e88492. doi: 10.7554/eLife.88492. PubMed |
| CaMKII autophosphorylation can occur between holoenzymes without subunit exchange. Lucic I, Heluin L, Jiang PL, Castro Scalise AG, Wang C, Franz A, Heyd F, Wahl MC, Liu F, Plested AJR. Elife. 2023 Aug 11;12:e86090. doi: 10.7554/eLife.86090. PubMed |
| Circularized Nanodiscs for Multivalent Mosaic Display of SARS-CoV-2 Spike Protein Antigens. Mabrouk MT, Zidan AA, Aly N, Mohammed MT, Ghantous F, Seaman MS, Lovell JF, Nasr ML. Vaccines (Basel). 2023 Oct 28;11(11):1655. doi: 10.3390/vaccines11111655. PubMed |
| Balancing the Nanoscale Organization in Multivalent Materials for Functional Inhibition of the Programmed Death-1 Immune Checkpoint. Paloja K, Weiden J, Hellmeier J, Eklund AS, Reinhardt SCM, Parish IA, Jungmann R, Bastings MMC. ACS Nano. 2024 Jan 16;18(2):1381-1395. doi: 10.1021/acsnano.3c06552. Epub 2023 Dec 21. PubMed |
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