Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3.
Singh AK, Krezel A
Microb Cell Fact. 2023 Jul 11;22(1):125. doi: 10.1186/s12934-023-02134-x. PubMed Article
Singh AK, Krezel A
Microb Cell Fact. 2023 Jul 11;22(1):125. doi: 10.1186/s12934-023-02134-x. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 200303 | SUMO_MT3 | Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility |
| 200304 | SUMO_LPETG_MT3 | Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage |
| 200305 | eSrtA-MT3 | Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA |
| 200306 | eSrtA-MT4 | Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA |