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pGP-CMV-GCaMP6m Citations (49)

Originally described in: Ultrasensitive fluorescent proteins for imaging neuronal activity.
Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354.
PubMed Journal

Articles Citing pGP-CMV-GCaMP6m

Measuring baseline Ca(2+) levels in subcellular compartments using genetically engineered fluorescent indicators. Hill JM, De Stefani D, Jones AW, Ruiz A, Rizzuto R, Szabadkai G. Methods Enzymol. 2014;543:47-72. doi: 10.1016/B978-0-12-801329-8.00003-9. PubMed
TPC2 mediates new mechanisms of platelet dense granule membrane dynamics through regulation of Ca2+ release. Ambrosio AL, Boyle JA, Di Pietro SM. Mol Biol Cell. 2015 Sep 15;26(18):3263-74. doi: 10.1091/mbc.E15-01-0058. Epub 2015 Jul 22. PubMed

Associated Plasmids

Multi-channel fiber photometry for population neuronal activity recording. Guo Q, Zhou J, Feng Q, Lin R, Gong H, Luo Q, Zeng S, Luo M, Fu L. Biomed Opt Express. 2015 Sep 10;6(10):3919-31. doi: 10.1364/BOE.6.003919. eCollection 2015 Oct 1. PubMed
A comparison of fluorescent Ca(2+) indicators for imaging local Ca(2+) signals in cultured cells. Lock JT, Parker I, Smith IF. Cell Calcium. 2015 Dec;58(6):638-48. doi: 10.1016/j.ceca.2015.10.003. Epub 2015 Oct 29. PubMed
Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. He L, Zhang Y, Ma G, Tan P, Li Z, Zang S, Wu X, Jing J, Fang S, Zhou L, Wang Y, Huang Y, Hogan PG, Han G, Zhou Y. Elife. 2015 Dec 8;4. pii: e10024. doi: 10.7554/eLife.10024. PubMed

Associated Plasmids

Serotonin neurons in the dorsal raphe nucleus encode reward signals. Li Y, Zhong W, Wang D, Feng Q, Liu Z, Zhou J, Jia C, Hu F, Zeng J, Guo Q, Fu L, Luo M. Nat Commun. 2016 Jan 28;7:10503. doi: 10.1038/ncomms10503. PubMed
Nucleus accumbens D2R cells signal prior outcomes and control risky decision-making. Zalocusky KA, Ramakrishnan C, Lerner TN, Davidson TJ, Knutson B, Deisseroth K. Nature. 2016 Mar 31;531(7596):642-6. doi: 10.1038/nature17400. Epub 2016 Mar 23. PubMed
TPC2 controls pigmentation by regulating melanosome pH and size. Ambrosio AL, Boyle JA, Aradi AE, Christian KA, Di Pietro SM. Proc Natl Acad Sci U S A. 2016 May 17;113(20):5622-7. doi: 10.1073/pnas.1600108113. Epub 2016 May 2. PubMed

Associated Plasmids

Concurrent Imaging of Receptor Trafficking and Calcium Dynamics by Spinning Disk Confocal Microscopy. Larsen DD, Choy RW, Park M. Methods Mol Biol. 2017;1538:249-259. PubMed
Deciphering the molecular mechanism responsible for GCaMP6m's Ca2+-dependent change in fluorescence. Barnett LM, Hughes TE, Drobizhev M. PLoS One. 2017 Feb 9;12(2):e0170934. doi: 10.1371/journal.pone.0170934. eCollection 2017. PubMed
Learning shapes the aversion and reward responses of lateral habenula neurons. Wang D, Li Y, Feng Q, Guo Q, Zhou J, Luo M. Elife. 2017 May 31;6. pii: e23045. doi: 10.7554/eLife.23045. PubMed
Ancestral Circuits for the Coordinated Modulation of Brain State. Lovett-Barron M, Andalman AS, Allen WE, Vesuna S, Kauvar I, Burns VM, Deisseroth K. Cell. 2017 Nov 30;171(6):1411-1423.e17. doi: 10.1016/j.cell.2017.10.021. Epub 2017 Nov 2. PubMed
Activation of the dopaminergic pathway from VTA to the medial olfactory tubercle generates odor-preference and reward. Zhang Z, Liu Q, Wen P, Zhang J, Rao X, Zhou Z, Zhang H, He X, Li J, Zhou Z, Xu X, Zhang X, Luo R, Lv G, Li H, Cao P, Wang L, Xu F. Elife. 2017 Dec 18;6. pii: 25423. doi: 10.7554/eLife.25423. PubMed
Astrocytes expressing ALS-linked mutant FUS induce motor neuron death through release of tumor necrosis factor-alpha. Kia A, McAvoy K, Krishnamurthy K, Trotti D, Pasinelli P. Glia. 2018 May;66(5):1016-1033. doi: 10.1002/glia.23298. Epub 2018 Jan 30. PubMed
Hypothalamic Circuits for Predation and Evasion. Li Y, Zeng J, Zhang J, Yue C, Zhong W, Liu Z, Feng Q, Luo M. Neuron. 2018 Feb 21;97(4):911-924.e5. doi: 10.1016/j.neuron.2018.01.005. Epub 2018 Feb 1. PubMed
Divergent midbrain circuits orchestrate escape and freezing responses to looming stimuli in mice. Shang C, Chen Z, Liu A, Li Y, Zhang J, Qu B, Yan F, Zhang Y, Liu W, Liu Z, Guo X, Li D, Wang Y, Cao P. Nat Commun. 2018 Mar 26;9(1):1232. doi: 10.1038/s41467-018-03580-7. PubMed
Assessment of cytosolic free calcium changes during ceramide-induced cell death in MDA-MB-231 breast cancer cells expressing the calcium sensor GCaMP6m. Bassett JJ, Bong AHL, Janke EK, Robitaille M, Roberts-Thomson SJ, Peters AA, Monteith GR. Cell Calcium. 2018 Jun;72:39-50. doi: 10.1016/j.ceca.2018.02.003. Epub 2018 Mar 2. PubMed
Astrocytic Ca(2+) responses in the spinal dorsal horn by noxious stimuli to the skin. Yoshihara K, Matsuda T, Kohro Y, Tozaki-Saitoh H, Inoue K, Tsuda M. J Pharmacol Sci. 2018 May;137(1):101-104. doi: 10.1016/j.jphs.2018.04.007. Epub 2018 May 3. PubMed
A Critical Neurodevelopmental Role for L-Type Voltage-Gated Calcium Channels in Neurite Extension and Radial Migration. Kamijo S, Ishii Y, Horigane SI, Suzuki K, Ohkura M, Nakai J, Fujii H, Takemoto-Kimura S, Bito H. J Neurosci. 2018 Jun 13;38(24):5551-5566. doi: 10.1523/JNEUROSCI.2357-17.2018. Epub 2018 May 17. PubMed
Probing the gating mechanism of the mechanosensitive channel Piezo1 with the small molecule Yoda1. Lacroix JJ, Botello-Smith WM, Luo Y. Nat Commun. 2018 May 23;9(1):2029. doi: 10.1038/s41467-018-04405-3. PubMed
Unsupervised Discovery of Demixed, Low-Dimensional Neural Dynamics across Multiple Timescales through Tensor Component Analysis. Williams AH, Kim TH, Wang F, Vyas S, Ryu SI, Shenoy KV, Schnitzer M, Kolda TG, Ganguli S. Neuron. 2018 Jun 27;98(6):1099-1115.e8. doi: 10.1016/j.neuron.2018.05.015. Epub 2018 Jun 7. PubMed
Characterization of developmental and molecular factors underlying release heterogeneity at Drosophila synapses. Akbergenova Y, Cunningham KL, Zhang YV, Weiss S, Littleton JT. Elife. 2018 Jul 10;7. pii: 38268. doi: 10.7554/eLife.38268. PubMed
NAADP-dependent Ca(2+) signaling regulates Middle East respiratory syndrome-coronavirus pseudovirus translocation through the endolysosomal system. Gunaratne GS, Yang Y, Li F, Walseth TF, Marchant JS. Cell Calcium. 2018 Nov;75:30-41. doi: 10.1016/j.ceca.2018.08.003. Epub 2018 Aug 9. PubMed
Response dynamics of midbrain dopamine neurons and serotonin neurons to heroin, nicotine, cocaine, and MDMA. Wei C, Han X, Weng D, Feng Q, Qi X, Li J, Luo M. Cell Discov. 2018 Nov 6;4:60. doi: 10.1038/s41421-018-0060-z. eCollection 2018. PubMed
5-Azido-8-ethynyl-NAADP: A bifunctional, clickable photoaffinity probe for the identification of NAADP receptors. Gunaratne GS, Su P, Marchant JS, Slama JT, Walseth TF. Biochim Biophys Acta Mol Cell Res. 2018 Dec 4. pii: S0167-4889(18)30521-4. doi: 10.1016/j.bbamcr.2018.11.017. PubMed
TCR microclusters form spatially segregated domains and sequentially assemble in calcium-dependent kinetic steps. Yi J, Balagopalan L, Nguyen T, McIntire KM, Samelson LE. Nat Commun. 2019 Jan 17;10(1):277. doi: 10.1038/s41467-018-08064-2. PubMed
ORAI1 and ORAI3 in Breast Cancer Molecular Subtypes and the Identification of ORAI3 as a Hypoxia Sensitive Gene and a Regulator of Hypoxia Responses. Azimi I, Milevskiy MJG, Chalmers SB, Yapa KTDS, Robitaille M, Henry C, Baillie GJ, Thompson EW, Roberts-Thomson SJ, Monteith GR. Cancers (Basel). 2019 Feb 11;11(2). pii: cancers11020208. doi: 10.3390/cancers11020208. PubMed
Neurotrophin receptor tyrosine kinases regulated with near-infrared light. Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV. Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3. PubMed

Associated Plasmids

Sniffer cells for the detection of neural Angiotensin II in vitro. Farmer GE, Amune A, Bachelor ME, Duong P, Yuan JP, Cunningham JT. Sci Rep. 2019 Jun 19;9(1):8820. doi: 10.1038/s41598-019-45262-4. PubMed
Distinct Mechanisms for Visual and Motor-Related Astrocyte Responses in Mouse Visual Cortex. Slezak M, Kandler S, Van Veldhoven PP, Van den Haute C, Bonin V, Holt MG. Curr Biol. 2019 Sep 23;29(18):3120-3127.e5. doi: 10.1016/j.cub.2019.07.078. Epub 2019 Sep 5. PubMed
Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii. Vella SA, Calixto A, Asady B, Li ZH, Moreno SNJ. Methods Mol Biol. 2020;2071:187-207. doi: 10.1007/978-1-4939-9857-9_11. PubMed
Control of locomotor speed, arousal, and hippocampal theta rhythms by the nucleus incertus. Lu L, Ren Y, Yu T, Liu Z, Wang S, Tan L, Zeng J, Feng Q, Lin R, Liu Y, Guo Q, Luo M. Nat Commun. 2020 Jan 14;11(1):262. doi: 10.1038/s41467-019-14116-y. PubMed
Visible light excited ratiometric-GECIs for long-term in-cellulo monitoring of calcium signals. Li J, Wang L, Chen Y, Yang Y, Liu J, Liu K, Lee YT, He N, Zhou Y, Wang Y. Cell Calcium. 2020 May;87:102165. doi: 10.1016/j.ceca.2020.102165. Epub 2020 Jan 23. PubMed
Intraorganellar calcium imaging in Arabidopsis seedling roots using the GCaMP variants GCaMP6m and R-CEPIA1er. Luo J, Chen L, Huang F, Gao P, Zhao H, Wang Y, Han S. J Plant Physiol. 2020 Mar - Apr;246-247:153127. doi: 10.1016/j.jplph.2020.153127. Epub 2020 Jan 23. PubMed
An optical brain-to-brain interface supports rapid information transmission for precise locomotion control. Lu L, Wang R, Luo M. Sci China Life Sci. 2020 Jun;63(6):875-885. doi: 10.1007/s11427-020-1675-x. Epub 2020 Mar 20. PubMed
Reporter cell assay for human CD33 validated by specific antibodies and human iPSC-derived microglia. Wissfeld J, Mathews M, Mossad O, Picardi P, Cinti A, Redaelli L, Pradier L, Brustle O, Neumann H. Sci Rep. 2021 Jun 29;11(1):13462. doi: 10.1038/s41598-021-92434-2. PubMed
Signal strength controls the rate of polarization within CTLs during killing. Frazer GL, Gawden-Bone CM, Dieckmann NMG, Asano Y, Griffiths GM. J Cell Biol. 2021 Oct 4;220(10). pii: 212498. doi: 10.1083/jcb.202104093. Epub 2021 Jul 22. PubMed
KCTD8 and KCTD12 Facilitate Axonal Expression of GABA(B) Receptors in Habenula Cholinergic Neurons. Ren Y, Liu Y, Zheng S, Luo M. J Neurosci. 2022 Mar 2;42(9):1648-1665. doi: 10.1523/JNEUROSCI.1676-21.2021. Epub 2022 Jan 11. PubMed
Identification of a dihydropyridine scaffold that blocks ryanodine receptors. Gunaratne GS, Rebbeck RT, McGurran LM, Yan Y, Arzua T, Frolkis T, Sprague DJ, Bai X, Cornea RL, Walseth TF, Marchant JS. iScience. 2021 Dec 27;25(1):103706. doi: 10.1016/j.isci.2021.103706. eCollection 2022 Jan 21. PubMed
Learning-induced biases in the ongoing dynamics of sensory representations predict stimulus generalization. Aschauer DF, Eppler JB, Ewig L, Chambers AR, Pokorny C, Kaschube M, Rumpel S. Cell Rep. 2022 Feb 8;38(6):110340. doi: 10.1016/j.celrep.2022.110340. PubMed
Increased matrix stiffness suppresses ATP-induced sustained Ca(2+) influx in MDA-MB-231 breast cancer cells. So CL, Meinert C, Xia Q, Robitaille M, Roberts-Thomson SJ, Monteith GR. Cell Calcium. 2022 Jun;104:102569. doi: 10.1016/j.ceca.2022.102569. Epub 2022 Mar 2. PubMed
Mechanosensitive calcium flashes promote sustained RhoA activation during tight junction remodeling. Varadarajan S, Chumki SA, Stephenson RE, Misterovich ER, Wu JL, Dudley CE, Erofeev IS, Goryachev AB, Miller AL. J Cell Biol. 2022 Apr 4;221(4):e202105107. doi: 10.1083/jcb.202105107. Epub 2022 Mar 7. PubMed
Novel Fluorescence-Based High-Throughput FLIPR Assay Utilizing Membrane-Tethered Genetic Calcium Sensors to Identify T-Type Calcium Channel Modulators. Zhang YL, Moran SP, Allen A, Baez-Nieto D, Xu Q, Wang LA, Martenis WE, Sacher JR, Gale JP, Weiwer M, Wagner FF, Pan JQ. ACS Pharmacol Transl Sci. 2022 Feb 25;5(3):156-168. doi: 10.1021/acsptsci.1c00233. eCollection 2022 Mar 11. PubMed
Mitochondrial calcium uniporter stabilization preserves energetic homeostasis during Complex I impairment. Balderas E, Eberhardt DR, Lee S, Pleinis JM, Sommakia S, Balynas AM, Yin X, Parker MC, Maguire CT, Cho S, Szulik MW, Bakhtina A, Bia RD, Friederich MW, Locke TM, Van Hove JLK, Drakos SG, Sancak Y, Tristani-Firouzi M, Franklin S, Rodan AR, Chaudhuri D. Nat Commun. 2022 May 19;13(1):2769. doi: 10.1038/s41467-022-30236-4. PubMed

Associated Plasmids

Extracellular Hydraulic Resistance Enhances Cell Migration. Maity D, Bera K, Li Y, Ge Z, Ni Q, Konstantopoulos K, Sun SX. Adv Sci (Weinh). 2022 Oct;9(29):e2200927. doi: 10.1002/advs.202200927. Epub 2022 Aug 28. PubMed
A stable sensory map emerges from a dynamic equilibrium of neurons with unstable tuning properties. Chambers AR, Aschauer DF, Eppler JB, Kaschube M, Rumpel S. Cereb Cortex. 2022 Nov 23:bhac445. doi: 10.1093/cercor/bhac445. PubMed
Chlamydia trachomatis suppresses host cell store-operated Ca(2+) entry and inhibits NFAT/calcineurin signaling. Chamberlain NB, Dimond Z, Hackstadt T. Sci Rep. 2022 Dec 10;12(1):21406. doi: 10.1038/s41598-022-25786-y. PubMed
Convergent activation of two-pore channels mediated by the NAADP-binding proteins JPT2 and LSM12. Gunaratne GS, Brailoiu E, Kumar S, Yuan Y, Slama JT, Walseth TF, Patel S, Marchant JS. Sci Signal. 2023 Aug 22;16(799):eadg0485. doi: 10.1126/scisignal.adg0485. Epub 2023 Aug 22. PubMed
Progesterone receptor membrane component 1 facilitates Ca(2+) signal amplification between endosomes and the endoplasmic reticulum. Gunaratne GS, Kumar S, Lin-Moshier Y, Slama JT, Brailoiu E, Patel S, Walseth TF, Marchant JS. J Biol Chem. 2023 Dec;299(12):105378. doi: 10.1016/j.jbc.2023.105378. Epub 2023 Oct 20. PubMed

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