pLV-eGFP
(Plasmid #36083)

• Purpose: (Empty Backbone) 3rd generation lentiviral transfer plasmid
• Depositing Lab: Pantelis Tsoulfas
• Publication: Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10.1016/j.expneurol.2013.06.013. Epub 2013 Jun 20.
• Citations: 75 Articles

Backbone

• Vector backbone: pLenti-MP2
• Backbone size (bp): 6782
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: optimal growth in low salt LB (5gm NaCl)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Species: Synthetic
• Insert Size (bp): 711
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.