Purpose(Empty Backbone) 3rd generation lentiviral plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36086||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6782
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsoptimal growth in low salt LB (5gm NaCl)
Alt nameenhanced blue fluorescent protein, BFP, eB
Insert Size (bp)734
- Cloning method Unknown
- 5′ sequencing primer CMV fwd
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe Azurite was obtained from Addgene and is originally from Patrick Daugherty (Mena et al., Nat Biotech 2006).
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the Miami project.
Azurite is mammalian codon optimized.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLV-Azurite was a gift from Pantelis Tsoulfas (Addgene plasmid # 36086 ; http://n2t.net/addgene:36086 ; RRID:Addgene_36086)