Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

CROPseq-Guide-Puro Citations (21)

Originally described in: Pooled CRISPR screening with single-cell transcriptome readout.
Datlinger P, Rendeiro AF, Schmidl C, Krausgruber T, Traxler P, Klughammer J, Schuster LC, Kuchler A, Alpar D, Bock C Nat Methods. 2017 Jan 18. doi: 10.1038/nmeth.4177. PubMed Journal

Articles Citing CROPseq-Guide-Puro

Articles
On the design of CRISPR-based single-cell molecular screens. Hill AJ, McFaline-Figueroa JL, Starita LM, Gasperini MJ, Matreyek KA, Packer J, Jackson D, Shendure J, Trapnell C. Nat Methods. 2018 Feb 19. pii: nmeth.4604. doi: 10.1038/nmeth.4604. PubMed

Associated Plasmids
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson A. Cell. 2018 Dec 13;175(7):1958-1971.e15. doi: 10.1016/j.cell.2018.10.024. Epub 2018 Nov 15. PubMed
Experimental and Computational Approaches for Single-Cell Enhancer Perturbation Assay. Xie S, Hon GC. Methods Mol Biol. 2019;1935:203-221. doi: 10.1007/978-1-4939-9057-3_14. PubMed
Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Genga RMJ, Kernfeld EM, Parsi KM, Parsons TJ, Ziller MJ, Maehr R. Cell Rep. 2019 Apr 16;27(3):708-718.e10. doi: 10.1016/j.celrep.2019.03.076. PubMed

Associated Plasmids
CRISPR Screening in Single Cells. Henriksson J. Methods Mol Biol. 2019;1979:395-406. doi: 10.1007/978-1-4939-9240-9_23. PubMed
CRISPR Interference-Based Platform for Multimodal Genetic Screens in Human iPSC-Derived Neurons. Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, Kampmann M. Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 10.1016/j.neuron.2019.07.014. PubMed

Associated Plasmids
Optical Pooled Screens in Human Cells. Feldman D, Singh A, Schmid-Burgk JL, Carlson RJ, Mezger A, Garrity AJ, Zhang F, Blainey PC. Cell. 2019 Oct 17;179(3):787-799.e17. doi: 10.1016/j.cell.2019.09.016. PubMed
Genome-scale CRISPR screens are efficient in non-homologous end-joining deficient cells. Ferreira da Silva J, Salic S, Wiedner M, Datlinger P, Essletzbichler P, Hanzl A, Superti-Furga G, Bock C, Winter G, Loizou JI. Sci Rep. 2019 Oct 31;9(1):15751. doi: 10.1038/s41598-019-52078-9. PubMed
Global Analysis of Enhancer Targets Reveals Convergent Enhancer-Driven Regulatory Modules. Xie S, Armendariz D, Zhou P, Duan J, Hon GC. Cell Rep. 2019 Nov 26;29(9):2570-2578.e5. doi: 10.1016/j.celrep.2019.10.073. PubMed
Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells. Jun S, Lim H, Chun H, Lee JH, Bang D. Commun Biol. 2020 Apr 2;3(1):154. doi: 10.1038/s42003-020-0888-2. PubMed
High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Gier RA, Budinich KA, Evitt NH, Cao Z, Freilich ES, Chen Q, Qi J, Lan Y, Kohli RM, Shi J. Nat Commun. 2020 Jul 13;11(1):3455. doi: 10.1038/s41467-020-17209-1. PubMed

Associated Plasmids
Pooled protein tagging, cellular imaging, and in situ sequencing for monitoring drug action in real time. Reicher A, Koren A, Kubicek S. Genome Res. 2020 Dec;30(12):1846-1855. doi: 10.1101/gr.261503.120. Epub 2020 Nov 17. PubMed
Characterizing the molecular regulation of inhibitory immune checkpoints with multimodal single-cell screens. Papalexi E, Mimitou EP, Butler AW, Foster S, Bracken B, Mauck WM 3rd, Wessels HH, Hao Y, Yeung BZ, Smibert P, Satija R. Nat Genet. 2021 Mar;53(3):322-331. doi: 10.1038/s41588-021-00778-2. Epub 2021 Mar 1. PubMed
Profiling the genetic determinants of chromatin accessibility with scalable single-cell CRISPR screens. Liscovitch-Brauer N, Montalbano A, Deng J, Mendez-Mancilla A, Wessels HH, Moss NG, Kung CY, Sookdeo A, Guo X, Geller E, Jaini S, Smibert P, Sanjana NE. Nat Biotechnol. 2021 Apr 29. pii: 10.1038/s41587-021-00902-x. doi: 10.1038/s41587-021-00902-x. PubMed

Associated Plasmids
Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing. Datlinger P, Rendeiro AF, Boenke T, Senekowitsch M, Krausgruber T, Barreca D, Bock C. Nat Methods. 2021 Jun;18(6):635-642. doi: 10.1038/s41592-021-01153-z. Epub 2021 May 31. PubMed
Pooled genetic perturbation screens with image-based phenotypes. Feldman D, Funk L, Le A, Carlson RJ, Leiken MD, Tsai F, Soong B, Singh A, Blainey PC. Nat Protoc. 2022 Feb;17(2):476-512. doi: 10.1038/s41596-021-00653-8. Epub 2022 Jan 12. PubMed
CRISPR activation and interference screens decode stimulation responses in primary human T cells. Schmidt R, Steinhart Z, Layeghi M, Freimer JW, Bueno R, Nguyen VQ, Blaeschke F, Ye CJ, Marson A. Science. 2022 Feb 4;375(6580):eabj4008. doi: 10.1126/science.abj4008. Epub 2022 Feb 4. PubMed

Associated Plasmids
Computational identification of clonal cells in single-cell CRISPR screens. Wang Y, Xie S, Armendariz D, Hon GC. BMC Genomics. 2022 Feb 15;23(1):135. doi: 10.1186/s12864-022-08359-1. PubMed
A time-resolved, multi-symbol molecular recorder via sequential genome editing. Choi J, Chen W, Minkina A, Chardon FM, Suiter CC, Regalado SG, Domcke S, Hamazaki N, Lee C, Martin B, Daza RM, Shendure J. Nature. 2022 Jul 6. pii: 10.1038/s41586-022-04922-8. doi: 10.1038/s41586-022-04922-8. PubMed

Associated Plasmids
Runx2 regulates chromatin accessibility to direct the osteoblast program at neonatal stages. Hojo H, Saito T, He X, Guo Q, Onodera S, Azuma T, Koebis M, Nakao K, Aiba A, Seki M, Suzuki Y, Okada H, Tanaka S, Chung UI, McMahon AP, Ohba S. Cell Rep. 2022 Sep 6;40(10):111315. doi: 10.1016/j.celrep.2022.111315. PubMed
Single-cell CRISPR immune screens reveal immunological roles of tumor intrinsic factors. Hou J, Liang S, Xu C, Wei Y, Wang Y, Tan Y, Sahni N, McGrail DJ, Bernatchez C, Davies M, Li Y, Chen R, Yi SS, Chen Y, Yee C, Chen K, Peng W. NAR Cancer. 2022 Dec 9;4(4):zcac038. doi: 10.1093/narcan/zcac038. eCollection 2022 Dec. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.