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Addgene

Intron-Tagging-EGFP-Frame0-Parental-Minicircle
(Plasmid #216124)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 216124 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMC.BESPX
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It is neccesary to remove plasmid backbone with minicircle technology (https://www.systembio.com/products/gene-expression-systems/minicircle-technology)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Intron-Tagging-EGFP-Frame0-Parental-Minicircle was a gift from Stefan Kubicek (Addgene plasmid # 216124 ; http://n2t.net/addgene:216124 ; RRID:Addgene_216124)

  • For your References section:

    Pooled multicolour tagging for visualizing subcellular protein dynamics. Reicher A, Reinis J, Ciobanu M, Ruzicka P, Malik M, Siklos M, Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S. Nat Cell Biol. 2024 May;26(5):745-756. doi: 10.1038/s41556-024-01407-w. Epub 2024 Apr 19. 10.1038/s41556-024-01407-w PubMed 38641660
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