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Pooled multicolour tagging for visualizing subcellular protein dynamics.

Reicher A, Reiniš J, Ciobanu M, Růžička P, Malik M, Siklos M, Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S
Nat Cell Biol. 2024 May;26(5):745-756. doi: 10.1038/s41556-024-01407-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216123CROPseq-Guide-BSDMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
216124Intron-Tagging-EGFP-Frame0-Parental-MinicircleParental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.
216125Intron-Tagging-mScarlet-Frame1-Parental-MinicircleParental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.
216126lenti-mTagBFP2Lentiviral vector for expressing mTagBFP2.
216127lenti-ER-mTagBFP2Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.
216128lenti-NLS-mTagBFP2Lentiviral vector for expressing mTagBFP2 localizing to the nucleus
216129lenti-NES-mTagBFP2Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm
216130lenti-mito-mTagBFP2Lentiviral vector for expressing mTagBFP2 localizing to mitochondria.
216131lenti-NLS-miRFP670nano-miRFP670Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.
216132lenti-mAmetrine-CAAXLentiviral vector for expressing mAmetrine localizing to the plasma membrane.
216133Human Genome-wide Intron Tagging Library, Frame 0Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.
216134Human Genome-wide Intron Tagging Library, Frame 1Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach.

Antibodies from Article