Pooled multicolour tagging for visualizing subcellular protein dynamics
Reicher A, Reiniš J, Ciobanu M, Růžička P, Malik M, Siklos M, Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S.
Nature Cell Biology (2024) Article
Reicher A, Reiniš J, Ciobanu M, Růžička P, Malik M, Siklos M, Kartysh V, Tomek T, Koren A, Rendeiro AF, Kubicek S.
Nature Cell Biology (2024) Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 216123 | CROPseq-Guide-BSD | Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter. |
| 216124 | Intron-Tagging-EGFP-Frame0-Parental-Minicircle | Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes. |
| 216125 | Intron-Tagging-mScarlet-Frame1-Parental-Minicircle | Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes. |
| 216126 | lenti-mTagBFP2 | Lentiviral vector for expressing mTagBFP2. |
| 216127 | lenti-ER-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum. |
| 216128 | lenti-NLS-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to the nucleus |
| 216129 | lenti-NES-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to the cytoplasm |
| 216130 | lenti-mito-mTagBFP2 | Lentiviral vector for expressing mTagBFP2 localizing to mitochondria. |
| 216131 | lenti-NLS-miRFP670nano-miRFP670 | Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus. |
| 216132 | lenti-mAmetrine-CAAX | Lentiviral vector for expressing mAmetrine localizing to the plasma membrane. |
| 216133 | Human Genome-wide Intron Tagging Library, Frame 0 | Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach. |
| 216134 | Human Genome-wide Intron Tagging Library, Frame 1 | Library of intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach. |