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Pooled CRISPR screening with single-cell transcriptome readout.
Datlinger P, Rendeiro AF, Schmidl C, Krausgruber T, Traxler P, Klughammer J, Schuster LC, Kuchler A, Alpar D, Bock C
Nat Methods. 2017 Jan 18. doi: 10.1038/nmeth.4177.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
86708CROPseq-Guide-PuroMakes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.