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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64
(Plasmid #180263)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 180263 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    LentiCRISPRv2
  • Total vector size (bp) 13123
  • Modifications to backbone
    The promoter was switched to SFFV, an mCherry-P2A was introduced upstream of dCas9-VP64, and the sgRNA cassette was removed.
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    S. Pyogenes
  • Mutation
    Nuclease dead Cas9
  • Promoter SFFV

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    dCas9-VP64 originated from lentiSAMv2 (Addgene #75112) and cloned into the lentiCRISPRv2-dCas9 backbone (Addgene #112233).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64 was a gift from Alexander Marson (Addgene plasmid # 180263 ; http://n2t.net/addgene:180263 ; RRID:Addgene_180263)
  • For your References section:

    CRISPR activation and interference screens decode stimulation responses in primary human T cells. Schmidt R, Steinhart Z, Layeghi M, Freimer JW, Bueno R, Nguyen VQ, Blaeschke F, Ye CJ, Marson A. Science. 2022 Feb 4;375(6580):eabj4008. doi: 10.1126/science.abj4008. Epub 2022 Feb 4. 10.1126/science.abj4008 PubMed 35113687