Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

sgRNA(MS2) cloning backbone Citations (27)

Plasmid Article: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136.
PubMed Journal

Articles Citing sgRNA(MS2) cloning backbone

Articles
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed
Manipulating the Prion Protein Gene Sequence and Expression Levels with CRISPR/Cas9. Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS. PLoS One. 2016 Apr 29;11(4):e0154604. doi: 10.1371/journal.pone.0154604. eCollection 2016. PubMed

Associated Plasmids

CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci. Fu Y, Rocha PP, Luo VM, Raviram R, Deng Y, Mazzoni EO, Skok JA. Nat Commun. 2016 May 25;7:11707. doi: 10.1038/ncomms11707. PubMed
Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems. Bialek JK, Dunay GA, Voges M, Schafer C, Spohn M, Stucka R, Hauber J, Lange UC. PLoS One. 2016 Jun 24;11(6):e0158294. doi: 10.1371/journal.pone.0158294. eCollection 2016. PubMed
Inhibition of histone deacetylase reduces transcription of NADPH oxidases and ROS production and ameliorates pulmonary arterial hypertension. Chen F, Li X, Aquadro E, Haigh S, Zhou J, Stepp DW, Weintraub NL, Barman SA, Fulton DJ. Free Radic Biol Med. 2016 Oct;99:167-178. doi: 10.1016/j.freeradbiomed.2016.08.003. Epub 2016 Aug 3. PubMed
Epigenetic Regulation of Interleukin 6 by Histone Acetylation in Macrophages and Its Role in Paraquat-Induced Pulmonary Fibrosis. Hu L, Yu Y, Huang H, Fan H, Hu L, Yin C, Li K, Fulton DJ, Chen F. Front Immunol. 2017 Jan 30;7:696. doi: 10.3389/fimmu.2016.00696. eCollection 2016. PubMed
Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs. Ferry QR, Lyutova R, Fulga TA. Nat Commun. 2017 Mar 3;8:14633. doi: 10.1038/ncomms14633. PubMed
The long noncoding RNA Wisper controls cardiac fibrosis and remodeling. Micheletti R, Plaisance I, Abraham BJ, Sarre A, Ting CC, Alexanian M, Maric D, Maison D, Nemir M, Young RA, Schroen B, Gonzalez A, Ounzain S, Pedrazzini T. Sci Transl Med. 2017 Jun 21;9(395). pii: 9/395/eaai9118. doi: 10.1126/scitranslmed.aai9118. PubMed
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. PubMed

Associated Plasmids

Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA. Lundh M, Plucinska K, Isidor MS, Petersen PSS, Emanuelli B. Mol Metab. 2017 Oct;6(10):1313-1320. doi: 10.1016/j.molmet.2017.07.001. Epub 2017 Jul 8. PubMed
Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Kleinjan DA, Wardrope C, Nga Sou S, Rosser SJ. Nat Commun. 2017 Oct 30;8(1):1191. doi: 10.1038/s41467-017-01222-y. PubMed
Evaluating different DNA binding domains to modulate L1 ORF2p-driven site-specific retrotransposition events in human cells. Ade CM, Derbes RS, Wagstaff BJ, Linker SB, White TB, Deharo D, Belancio VP, Ivics Z, Roy-Engel AM. Gene. 2018 Feb 5;642:188-198. doi: 10.1016/j.gene.2017.11.033. Epub 2017 Nov 14. PubMed
A transcribed enhancer dictates mesendoderm specification in pluripotency. Alexanian M, Maric D, Jenkinson SP, Mina M, Friedman CE, Ting CC, Micheletti R, Plaisance I, Nemir M, Maison D, Kernen J, Pezzuto I, Villeneuve D, Burdet F, Ibberson M, Leib SL, Palpant NJ, Hernandez N, Ounzain S, Pedrazzini T. Nat Commun. 2017 Nov 27;8(1):1806. doi: 10.1038/s41467-017-01804-w. PubMed
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC. Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7. PubMed

Associated Plasmids

Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing. Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y. Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222. PubMed

Associated Plasmids

Activation of the Anti-Aging and Cognition-Enhancing Gene Klotho by CRISPR-dCas9 Transcriptional Effector Complex. Chen CD, Zeldich E, Li Y, Yuste A, Abraham CR. J Mol Neurosci. 2018 Feb;64(2):175-184. doi: 10.1007/s12031-017-1011-0. Epub 2018 Jan 19. PubMed
lncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperatures. Zhao Z, Senturk N, Song C, Grummt I. Genes Dev. 2018 Jun 1;32(11-12):836-848. doi: 10.1101/gad.311688.118. Epub 2018 Jun 15. PubMed
Biased genome editing using the local accumulation of DSB repair molecules system. Nakade S, Mochida K, Kunii A, Nakamae K, Aida T, Tanaka K, Sakamoto N, Sakuma T, Yamamoto T. Nat Commun. 2018 Aug 16;9(1):3270. doi: 10.1038/s41467-018-05773-6. PubMed
CRISPRa-mediated NEAT1 lncRNA upregulation induces formation of intact paraspeckles. Yamazaki T, Fujikawa C, Kubota A, Takahashi A, Hirose T. Biochem Biophys Res Commun. 2018 Sep 26;504(1):218-224. doi: 10.1016/j.bbrc.2018.08.158. Epub 2018 Sep 1. PubMed
Expanding the repertoire of glucocorticoid receptor target genes by engineering genomic response elements. Thormann V, Glaser LV, Rothkegel MC, Borschiwer M, Bothe M, Fuchs A, Meijsing SH. Life Sci Alliance. 2019 Mar 13;2(2). pii: 2/2/e201800283. doi: 10.26508/lsa.201800283. Print 2019 Apr. PubMed
CRISPRai for simultaneous gene activation and inhibition to promote stem cell chondrogenesis and calvarial bone regeneration. Truong VA, Hsu MN, Kieu Nguyen NT, Lin MW, Shen CC, Lin CY, Hu YC. Nucleic Acids Res. 2019 Jul 26;47(13):e74. doi: 10.1093/nar/gkz267. PubMed
Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes. Ferdosi SR, Ewaisha R, Moghadam F, Krishna S, Park JG, Ebrahimkhani MR, Kiani S, Anderson KS. Nat Commun. 2019 Apr 23;10(1):1842. doi: 10.1038/s41467-019-09693-x. PubMed
A High-Throughput Platform to Identify Small-Molecule Inhibitors of CRISPR-Cas9. Maji B, Gangopadhyay SA, Lee M, Shi M, Wu P, Heler R, Mok B, Lim D, Siriwardena SU, Paul B, Dancik V, Vetere A, Mesleh MF, Marraffini LA, Liu DR, Clemons PA, Wagner BK, Choudhary A. Cell. 2019 May 2;177(4):1067-1079.e19. doi: 10.1016/j.cell.2019.04.009. PubMed
Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors. Tran NT, Bashir S, Li X, Rossius J, Chu VT, Rajewsky K, Kuhn R. Front Genet. 2019 Apr 30;10:365. doi: 10.3389/fgene.2019.00365. eCollection 2019. PubMed
The lncRNA Locus Handsdown Regulates Cardiac Gene Programs and Is Essential for Early Mouse Development. Ritter N, Ali T, Kopitchinski N, Schuster P, Beisaw A, Hendrix DA, Schulz MH, Muller-McNicoll M, Dimmeler S, Grote P. Dev Cell. 2019 Sep 9;50(5):644-657.e8. doi: 10.1016/j.devcel.2019.07.013. Epub 2019 Aug 15. PubMed
CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration. Hsu MN, Liao HT, Truong VA, Huang KL, Yu FJ, Chen HH, Nguyen TKN, Makarevich P, Parfyonova Y, Hu YC. Theranostics. 2019 Aug 14;9(21):6099-6111. doi: 10.7150/thno.36790. eCollection 2019. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.