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Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation.
Erdel F, Rademacher A, Vlijm R, Tunnermann J, Frank L, Weinmann R, Schweigert E, Yserentant K, Hummert J, Bauer C, Schumacher S, Al Alwash A, Normand C, Herten DP, Engelhardt J, Rippe K
Mol Cell. 2020 Apr 16;78(2):236-249.e7. doi: 10.1016/j.molcel.2020.02.005. Epub 2020 Feb 25.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
181883pPHR-mCherry-mHP1alphaSoluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers
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181884pPHR-mCherry-hHP1alphaSoluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizers
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181885pPHR-mCherry-hHP1gammaSoluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers
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181886pPHR-mCherry-hNCLSoluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers
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181887pPHR-mCherry-hNPMSoluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers
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181888pPHR-mCherry-MECP2Soluble light-dependent effector protein (Methyl-CpG binding protein) for optodroplet formation or recruitment to CIBN localizers
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181889pPHR-mCherry-mHP1alpha-I163ASoluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers,
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181890pPHR-mCherry-nE-mHP1alphaSoluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers
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181891pPHR-mCherry-nE-mHP1alpha-delCTESoluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers
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181892pPHR-mCherry-FUSN-mHP1alphaSoluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers
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181893pPHR-mCherry-RGG-GFP-RGG-mHP1alphaSoluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers
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181894pPHR-mCherry-PGL-1-mHP1alphaSoluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers
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181895pGFP-nE-mHP1alphaFluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates
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181896pGFP-nE-mHP1alpha-delCTEFluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion
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181897pGFP-FUSN-mHP1alphaFluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)
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181898pRGG-GFP-RGG-mHP1alphaFluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1
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181899pGFP-PGL-1-mHP1alphaFluorescently tagged HP1alpha fused to PGL-1
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181900pGFP-mHP1alphaFluorescently tagged HP1alpha
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181901pGFP-mHP1betaFluorescently tagged HP1beta
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181902pGFP-mHP1gammaFluorescently tagged HP1gamma
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181903pGFP-hPMLIIIFluorescently tagged PMLIII
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181904pMECP2-GFPFluorescently tagged MECP2, GFP
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181905pMECP2-RFPFluorescently tagged MECP2, RFP
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181906pdCas9-GFPFluorescently tagged dead Cas9
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181907pdCas9-GFP-VPRFluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta
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181908psgRNA-2xMS2-mSATSingle guide RNA with 2XMS2 loops targeting mouse major satellite repeats
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181909psgRNA-2xMS2-lacOSingle guide RNA with 2XMS2 loops targeting bacterial lac operator
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181910pET16-mHP1alphaBacterial protein expression vector for HP1alpha
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181911pET28-mHP1alphaBacterial protein expression vector for HP1alpha
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181912pET28-GFP-mHP1alphaBacterial protein expression vector for GFP-tagged HP1alpha
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181913pTRE3G-ZSGreen1-mHP1alphaInducible mammalian expression vector for ZsGreen1-tagged HP1alpha
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Recombinant Antibodies from Article