Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation.
Erdel F, Rademacher A, Vlijm R, Tunnermann J, Frank L, Weinmann R, Schweigert E, Yserentant K, Hummert J, Bauer C, Schumacher S, Al Alwash A, Normand C, Herten DP, Engelhardt J, Rippe K
Mol Cell. 2020 Apr 16;78(2):236-249.e7. doi: 10.1016/j.molcel.2020.02.005. Epub 2020 Feb 25. PubMed Article
Erdel F, Rademacher A, Vlijm R, Tunnermann J, Frank L, Weinmann R, Schweigert E, Yserentant K, Hummert J, Bauer C, Schumacher S, Al Alwash A, Normand C, Herten DP, Engelhardt J, Rippe K
Mol Cell. 2020 Apr 16;78(2):236-249.e7. doi: 10.1016/j.molcel.2020.02.005. Epub 2020 Feb 25. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 181883 | pPHR-mCherry-mHP1alpha | Soluble light-dependent effector protein (mouse HP1alpha) for optodroplet formation or recruitment to CIBN localizers |
| 181884 | pPHR-mCherry-hHP1alpha | Soluble light-dependent effector protein (human HP1alpha) for optodroplet formation or recruitment to CIBN localizers |
| 181885 | pPHR-mCherry-hHP1gamma | Soluble light-dependent effector protein (human HP1gamma) for optodroplet formation or recruitment to CIBN localizers |
| 181886 | pPHR-mCherry-hNCL | Soluble light-dependent effector protein (nucleolin) for optodroplet formation or recruitment to CIBN localizers |
| 181887 | pPHR-mCherry-hNPM | Soluble light-dependent effector protein (nucleophosmin) for optodroplet formation or recruitment to CIBN localizers |
| 181888 | pPHR-mCherry-MECP2 | Soluble light-dependent effector protein (Methyl-CpG binding protein) for optodroplet formation or recruitment to CIBN localizers |
| 181889 | pPHR-mCherry-mHP1alpha-I163A | Soluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers, |
| 181890 | pPHR-mCherry-nE-mHP1alpha | Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant) for optodroplet formation or recruitment to CIBN localizers |
| 181891 | pPHR-mCherry-nE-mHP1alpha-delCTE | Soluble light-dependent effector protein (HP1alpha, phosphomimetic NTE mutant with deletion of C-terminal extension)for optodroplet formation or recruitment to CIBN localizers |
| 181892 | pPHR-mCherry-FUSN-mHP1alpha | Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers |
| 181893 | pPHR-mCherry-RGG-GFP-RGG-mHP1alpha | Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers |
| 181894 | pPHR-mCherry-PGL-1-mHP1alpha | Soluble light-dependent effector protein (HP1alpha fused to PGL-1) for optodroplet formation or recruitment to CIBN localizers |
| 181895 | pGFP-nE-mHP1alpha | Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates |
| 181896 | pGFP-nE-mHP1alpha-delCTE | Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion |
| 181897 | pGFP-FUSN-mHP1alpha | Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) |
| 181898 | pRGG-GFP-RGG-mHP1alpha | Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1 |
| 181899 | pGFP-PGL-1-mHP1alpha | Fluorescently tagged HP1alpha fused to PGL-1 |
| 181900 | pGFP-mHP1alpha | Fluorescently tagged HP1alpha |
| 181901 | pGFP-mHP1beta | Fluorescently tagged HP1beta |
| 181902 | pGFP-mHP1gamma | Fluorescently tagged HP1gamma |
| 181903 | pGFP-hPMLIII | Fluorescently tagged PMLIII |
| 181904 | pMECP2-GFP | Fluorescently tagged MECP2, GFP |
| 181905 | pMECP2-RFP | Fluorescently tagged MECP2, RFP |
| 181906 | pdCas9-GFP | Fluorescently tagged dead Cas9 |
| 181907 | pdCas9-GFP-VPR | Fluorescently tagged dead Cas9 fused to the tripartite transcriptional activator VP64-p65-Rta |
| 181908 | psgRNA-2xMS2-mSAT | Single guide RNA with 2XMS2 loops targeting mouse major satellite repeats |
| 181909 | psgRNA-2xMS2-lacO | Single guide RNA with 2XMS2 loops targeting bacterial lac operator |
| 181910 | pET16-mHP1alpha | Bacterial protein expression vector for HP1alpha |
| 181911 | pET28-mHP1alpha | Bacterial protein expression vector for HP1alpha |
| 181912 | pET28-GFP-mHP1alpha | Bacterial protein expression vector for GFP-tagged HP1alpha |
| 181913 | pTRE3G-ZSGreen1-mHP1alpha | Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha |