Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Holiday Schedule: Addgene will be closed November 28th & 29th for the Thanksgiving holiday. Order processing and shipping may be delayed the week of November 25th - 29th. If you have any questions, please contact us at [email protected].

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

G-CaMP3 Citations (55)

Plasmid Article: Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators.
Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, Petreanu L, Akerboom J, McKinney SA, Schreiter ER, Bargmann CI, Jayaraman V, Svoboda K, Looger LL. Nat Methods. 2009 Dec;6(12):875-81.
PubMed Journal

Articles Citing G-CaMP3

Articles
Unfolded proteins are Ire1-activating ligands that directly induce the unfolded protein response. Gardner BM, Walter P. Science. 2011 Sep 30;333(6051):1891-4. doi: 10.1126/science.1209126. Epub 2011 Aug 18. PubMed
An Expanded Palette of Genetically Encoded Ca2+ Indicators. Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE. Science. 2011 Sep 8. PubMed

Associated Plasmids

Dynamic changes in neural circuit topology following mild mechanical injury in vitro. Patel TP, Ventre SC, Meaney DF. Ann Biomed Eng. 2012 Jan;40(1):23-36. doi: 10.1007/s10439-011-0390-6. Epub 2011 Oct 13. PubMed
Quantitative comparison of genetically encoded Ca indicators in cortical pyramidal cells and cerebellar Purkinje cells. Yamada Y, Michikawa T, Hashimoto M, Horikawa K, Nagai T, Miyawaki A, Hausser M, Mikoshiba K. Front Cell Neurosci. 2011 Sep 29;5:18. doi: 10.3389/fncel.2011.00018. eCollection 2011. PubMed
Concurrent imaging of synaptic vesicle recycling and calcium dynamics. Li H, Foss SM, Dobryy YL, Park CK, Hires SA, Shaner NC, Tsien RY, Osborne LC, Voglmaier SM. Front Mol Neurosci. 2011 Nov 2;4:34. doi: 10.3389/fnmol.2011.00034. eCollection 2011. PubMed
Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD. PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9. PubMed

Associated Plasmids

Akt2 inhibits the activation of NFAT in lymphocytes by modulating calcium release from intracellular stores. Martin VA, Wang WH, Lipchik AM, Parker LL, He Y, Zhang S, Zhang ZY, Geahlen RL. Cell Signal. 2012 May;24(5):1064-73. doi: 10.1016/j.cellsig.2012.01.001. Epub 2012 Jan 11. PubMed
Neural activity imaging with genetically encoded calcium indicators. Tian L, Akerboom J, Schreiter ER, Looger LL. Prog Brain Res. 2012;196:79-94. doi: 10.1016/B978-0-444-59426-6.00005-7. PubMed
Analysis of functional neuronal connectivity in the Drosophila brain. Yao Z, Macara AM, Lelito KR, Minosyan TY, Shafer OT. J Neurophysiol. 2012 Jul;108(2):684-96. doi: 10.1152/jn.00110.2012. Epub 2012 Apr 25. PubMed
Human ES-cell-derived cardiomyocytes electrically couple and suppress arrhythmias in injured hearts. Shiba Y, Fernandes S, Zhu WZ, Filice D, Muskheli V, Kim J, Palpant NJ, Gantz J, Moyes KW, Reinecke H, Van Biber B, Dardas T, Mignone JL, Izawa A, Hanna R, Viswanathan M, Gold JD, Kotlikoff MI, Sarvazyan N, Kay MW, Murry CE, Laflamme MA. Nature. 2012 Sep 13;489(7415):322-5. doi: 10.1038/nature11317. PubMed
Cholinergic coordination of presynaptic and postsynaptic activity induces timing-dependent hippocampal synaptic plasticity. Gu Z, Lamb PW, Yakel JL. J Neurosci. 2012 Sep 5;32(36):12337-48. doi: 10.1523/JNEUROSCI.2129-12.2012. PubMed
Quantitative comparison of novel GCaMP-type genetically encoded Ca(2+) indicators in mammalian neurons. Yamada Y, Mikoshiba K. Front Cell Neurosci. 2012 Oct 8;6:41. doi: 10.3389/fncel.2012.00041. eCollection 2012. PubMed
High yield derivation of enriched glutamatergic neurons from suspension-cultured mouse ESCs for neurotoxicology research. Hubbard KS, Gut IM, Lyman ME, Tuznik KM, Mesngon MT, McNutt PM. BMC Neurosci. 2012 Oct 24;13:127. doi: 10.1186/1471-2202-13-127. PubMed
TMHS is an integral component of the mechanotransduction machinery of cochlear hair cells. Xiong W, Grillet N, Elledge HM, Wagner TF, Zhao B, Johnson KR, Kazmierczak P, Muller U. Cell. 2012 Dec 7;151(6):1283-95. doi: 10.1016/j.cell.2012.10.041. PubMed
Dysfunction of KCNK potassium channels impairs neuronal migration in the developing mouse cerebral cortex. Bando Y, Hirano T, Tagawa Y. Cereb Cortex. 2014 Apr;24(4):1017-29. doi: 10.1093/cercor/bhs387. Epub 2012 Dec 12. PubMed
Calcium oscillations-coupled conversion of actin travelling waves to standing oscillations. Wu M, Wu X, De Camilli P. Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1339-44. doi: 10.1073/pnas.1221538110. Epub 2013 Jan 7. PubMed
Imaging burst kinetics and spatial coordination during serial killing by single natural killer cells. Choi PJ, Mitchison TJ. Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6488-93. doi: 10.1073/pnas.1221312110. Epub 2013 Apr 1. PubMed

Associated Plasmids

Imaging calcium microdomains within entire astrocyte territories and endfeet with GCaMPs expressed using adeno-associated viruses. Shigetomi E, Bushong EA, Haustein MD, Tong X, Jackson-Weaver O, Kracun S, Xu J, Sofroniew MV, Ellisman MH, Khakh BS. J Gen Physiol. 2013 Apr 15. PubMed

Associated Plasmids

Filamin and phospholipase C-epsilon are required for calcium signaling in the Caenorhabditis elegans spermatheca. Kovacevic I, Orozco JM, Cram EJ. PLoS Genet. 2013 May;9(5):e1003510. doi: 10.1371/journal.pgen.1003510. Epub 2013 May 9. PubMed
Modulation by Syk of Bcl-2, calcium and the calpain-calpastatin proteolytic system in human breast cancer cells. Fei B, Yu S, Geahlen RL. Biochim Biophys Acta. 2013 Oct;1833(10):2153-64. doi: 10.1016/j.bbamcr.2013.05.010. Epub 2013 May 16. PubMed
Transcription factors MYOCD, SRF, Mesp1 and SMARCD3 enhance the cardio-inducing effect of GATA4, TBX5, and MEF2C during direct cellular reprogramming. Christoforou N, Chellappan M, Adler AF, Kirkton RD, Wu T, Addis RC, Bursac N, Leong KW. PLoS One. 2013 May 21;8(5):e63577. doi: 10.1371/journal.pone.0063577. Print 2013. PubMed
In vivo imaging of alphaherpesvirus infection reveals synchronized activity dependent on axonal sorting of viral proteins. Granstedt AE, Bosse JB, Thiberge SY, Enquist LW. Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):E3516-25. doi: 10.1073/pnas.1311062110. Epub 2013 Aug 26. PubMed
Stochastic Ca(2+) waves that propagate through the neuroepithelium in limited distances of the brain and retina imaged with GCaMP3 in zebrafish embryos. Okamoto S, Nakagawa M, Hatta K. Zoolog Sci. 2013 Sep;30(9):716-23. doi: 10.2108/zsj.30.716. PubMed
Cilioplasm is a cellular compartment for calcium signaling in response to mechanical and chemical stimuli. Jin X, Mohieldin AM, Muntean BS, Green JA, Shah JV, Mykytyn K, Nauli SM. Cell Mol Life Sci. 2014 Jun;71(11):2165-78. doi: 10.1007/s00018-013-1483-1. Epub 2013 Oct 9. PubMed
Monitoring synaptic and neuronal activity in 3D with synthetic and genetic indicators using a compact acousto-optic lens two-photon microscope. Fernandez-Alfonso T, Nadella KM, Iacaruso MF, Pichler B, Ros H, Kirkby PA, Silver RA. J Neurosci Methods. 2014 Jan 30;222:69-81. doi: 10.1016/j.jneumeth.2013.10.021. Epub 2013 Nov 4. PubMed
Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis. Cai Z, Jitkaew S, Zhao J, Chiang HC, Choksi S, Liu J, Ward Y, Wu LG, Liu ZG. Nat Cell Biol. 2014 Jan;16(1):55-65. doi: 10.1038/ncb2883. Epub 2013 Dec 8. PubMed
Presynaptic alpha7 nicotinic acetylcholine receptors enhance hippocampal mossy fiber glutamatergic transmission via PKA activation. Cheng Q, Yakel JL. J Neurosci. 2014 Jan 1;34(1):124-33. doi: 10.1523/JNEUROSCI.2973-13.2014. PubMed
Multiparametric optical analysis of mitochondrial redox signals during neuronal physiology and pathology in vivo. Breckwoldt MO, Pfister FM, Bradley PM, Marinkovic P, Williams PR, Brill MS, Plomer B, Schmalz A, St Clair DK, Naumann R, Griesbeck O, Schwarzlander M, Godinho L, Bareyre FM, Dick TP, Kerschensteiner M, Misgeld T. Nat Med. 2014 May;20(5):555-60. doi: 10.1038/nm.3520. Epub 2014 Apr 20. PubMed
High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor. St-Pierre F, Marshall JD, Yang Y, Gong Y, Schnitzer MJ, Lin MZ. Nat Neurosci. 2014 Apr 22. doi: 10.1038/nn.3709. PubMed

Associated Plasmids

Polyunsaturated fatty acids inhibit stimulated coupling between the ER Ca(2+) sensor STIM1 and the Ca(2+) channel protein Orai1 in a process that correlates with inhibition of stimulated STIM1 oligomerization. Holowka D, Korzeniowski MK, Bryant KL, Baird B. Biochim Biophys Acta. 2014 Aug;1841(8):1210-6. doi: 10.1016/j.bbalip.2014.04.006. Epub 2014 Apr 24. PubMed
Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts. Chong JJ, Yang X, Don CW, Minami E, Liu YW, Weyers JJ, Mahoney WM, Van Biber B, Cook SM, Palpant NJ, Gantz JA, Fugate JA, Muskheli V, Gough GM, Vogel KW, Astley CA, Hotchkiss CE, Baldessari A, Pabon L, Reinecke H, Gill EA, Nelson V, Kiem HP, Laflamme MA, Murry CE. Nature. 2014 Jun 12;510(7504):273-7. doi: 10.1038/nature13233. Epub 2014 Apr 30. PubMed
Activation of Cdc42 is necessary for sustained oscillations of Ca2+ and PIP2 stimulated by antigen in RBL mast cells. Wilkes MM, Wilson JD, Baird B, Holowka D. Biol Open. 2014 Jul 4;3(8):700-10. doi: 10.1242/bio.20148862. PubMed
Methods for assessing the electromechanical integration of human pluripotent stem cell-derived cardiomyocyte grafts. Zhu WZ, Filice D, Palpant NJ, Laflamme MA. Methods Mol Biol. 2014;1181:229-47. doi: 10.1007/978-1-4939-1047-2_20. PubMed
Control of Spontaneous Ca2+ Transients Is Critical for Neuronal Maturation in the Developing Neocortex. Bando Y, Irie K, Shimomura T, Umeshima H, Kushida Y, Kengaku M, Fujiyoshi Y, Hirano T, Tagawa Y. Cereb Cortex. 2014 Aug 11. pii: bhu180. PubMed
Using injectoporation to deliver genes to mechanosensory hair cells. Xiong W, Wagner T, Yan L, Grillet N, Muller U. Nat Protoc. 2014 Oct;9(10):2438-49. doi: 10.1038/nprot.2014.168. Epub 2014 Sep 18. PubMed
TMIE is an essential component of the mechanotransduction machinery of cochlear hair cells. Zhao B, Wu Z, Grillet N, Yan L, Xiong W, Harkins-Perry S, Muller U. Neuron. 2014 Dec 3;84(5):954-67. doi: 10.1016/j.neuron.2014.10.041. Epub 2014 Nov 20. PubMed
Characterization and modeling of Ca(2+) oscillations in mouse primary mesothelial cells. Pecze L, Schwaller B. Biochim Biophys Acta. 2015 Mar;1854(3):632-45. doi: 10.1016/j.bbamcr.2014.12.025. Epub 2014 Dec 30. PubMed
Resveratrol up-regulates AMPA receptor expression via AMP-activated protein kinase-mediated protein translation. Wang G, Amato S, Gilbert J, Man HY. Neuropharmacology. 2015 Aug;95:144-53. doi: 10.1016/j.neuropharm.2015.03.003. Epub 2015 Mar 17. PubMed
Activation of alpha7 nicotinic acetylcholine receptors increases intracellular cAMP levels via activation of AC1 in hippocampal neurons. Cheng Q, Yakel JL. Neuropharmacology. 2015 Aug;95:405-14. doi: 10.1016/j.neuropharm.2015.04.016. Epub 2015 Apr 29. PubMed
Calcium Spikes in Epithelium: study on Drosophila early embryos. Markova O, Senatore S, Chardes C, Lenne PF. Sci Rep. 2015 Jul 22;5:11379. doi: 10.1038/srep11379. PubMed
Routes of Ca2+ Shuttling during Ca2+ Oscillations: FOCUS ON THE ROLE OF MITOCHONDRIAL Ca2+ HANDLING AND CYTOSOLIC Ca2+ BUFFERS. Pecze L, Blum W, Schwaller B. J Biol Chem. 2015 Nov 20;290(47):28214-30. doi: 10.1074/jbc.M115.663179. Epub 2015 Sep 22. PubMed
F-actin waves, actin cortex disassembly and focal exocytosis driven by actin-phosphoinositide positive feedback. Masters TA, Sheetz MP, Gauthier NC. Cytoskeleton (Hoboken). 2016 Apr;73(4):180-96. doi: 10.1002/cm.21287. Epub 2016 Apr 7. PubMed
The Severity of Acute Stress Is Represented by Increased Synchronous Activity and Recruitment of Hypothalamic CRH Neurons. Vom Berg-Maurer CM, Trivedi CA, Bollmann JH, De Marco RJ, Ryu S. J Neurosci. 2016 Mar 16;36(11):3350-62. doi: 10.1523/JNEUROSCI.3390-15.2016. PubMed
Characterization of a ligand-gated cation channel based on an inositol receptor in the silkworm, Bombyx mori. Kikuta S, Endo H, Tomita N, Takada T, Morita C, Asaoka K, Sato R. Insect Biochem Mol Biol. 2016 Jul;74:12-20. doi: 10.1016/j.ibmb.2016.04.010. Epub 2016 Apr 27. PubMed
Murine Fam65b forms ring-like structures at the base of stereocilia critical for mechanosensory hair cell function. Zhao B, Wu Z, Muller U. Elife. 2016 Jun 8;5. pii: e14222. doi: 10.7554/eLife.14222. PubMed
The effect of chronic alcohol consumption on mitochondrial calcium handling in hepatocytes. Wang G, Memin E, Murali I, Gaspers LD. Biochem J. 2016 Nov 1;473(21):3903-3921. Epub 2016 Aug 31. PubMed
Ganglionic GFAP + glial Gq-GPCR signaling enhances heart functions in vivo. Xie AX, Lee JJ, McCarthy KD. JCI Insight. 2017 Jan 26;2(2):e90565. doi: 10.1172/jci.insight.90565. PubMed
Substitution with a Single Cysteine in the Green Fluorescent Protein-Based Calcium Indicator GCaMP3 Enhances Calcium Sensitivity. Kim TJ, Yoo JY, Shim WS. J Fluoresc. 2017 Nov;27(6):2187-2193. doi: 10.1007/s10895-017-2159-2. Epub 2017 Aug 8. PubMed
Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb. Iwata R, Kiyonari H, Imai T. Neuron. 2017 Dec 6;96(5):1139-1152.e7. doi: 10.1016/j.neuron.2017.11.008. PubMed
Proteolytic Processing of Neurexins by Presenilins Sustains Synaptic Vesicle Release. Servian-Morilla E, Robles-Lanuza E, Sanchez-Hidalgo AC, Camacho-Garcia RJ, Paez-Gomez JA, Mavillard F, Saura CA, Martinez-Mir A, Scholl FG. J Neurosci. 2018 Jan 24;38(4):901-917. doi: 10.1523/JNEUROSCI.1357-17.2017. Epub 2017 Dec 11. PubMed
Efficient differentiation of cardiomyocytes and generation of calcium-sensor reporter lines from nonhuman primate iPSCs. Lin Y, Liu H, Klein M, Ostrominski J, Hong SG, Yada RC, Chen G, Navarengom K, Schwartzbeck R, San H, Yu ZX, Liu C, Linask K, Beers J, Qiu L, Dunbar CE, Boehm M, Zou J. Sci Rep. 2018 Apr 12;8(1):5907. doi: 10.1038/s41598-018-24074-y. PubMed
In vivo wide-field calcium imaging of mouse thalamocortical synapses with an 8 K ultra-high-definition camera. Yoshida E, Terada SI, Tanaka YH, Kobayashi K, Ohkura M, Nakai J, Matsuzaki M. Sci Rep. 2018 May 29;8(1):8324. doi: 10.1038/s41598-018-26566-3. PubMed
OFF-responses of interneurons optimize avoidance behaviors depending on stimulus strength via electrical synapses. Hori S, Oda S, Suehiro Y, Iino Y, Mitani S. PLoS Genet. 2018 Jun 25;14(6):e1007477. doi: 10.1371/journal.pgen.1007477. eCollection 2018 Jun. PubMed

Associated Plasmids

Super-wide-field two-photon imaging with a micro-optical device moving in post-objective space. Terada SI, Kobayashi K, Ohkura M, Nakai J, Matsuzaki M. Nat Commun. 2018 Sep 3;9(1):3550. doi: 10.1038/s41467-018-06058-8. PubMed
Developmental NMDA receptor dysregulation in the infantile neuronal ceroid lipofuscinosis mouse model. Koster KP, Francesconi W, Berton F, Alahmadi S, Srinivas R, Yoshii A. Elife. 2019 Apr 4;8. pii: 40316. doi: 10.7554/eLife.40316. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.