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MAP2-GCaMP3
(Plasmid #43917)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 43917 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    FU-MAP2-Gateway
  • Backbone manufacturer
    Addgene plasmid 43915
  • Backbone size w/o insert (bp) 9600
  • Total vector size (bp) 10900
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use recA cells, such as STBL3 (Invitrogen)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP3
  • Alt name
    G-CaMP3
  • Species
    Synthetic
  • Insert Size (bp)
    1300
  • Mutation
    E247V in GCaMP3
  • GenBank ID
    HM143847.1 ADJ53338.1
  • Promoter Human MAP2 promoter
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • T7 epitope (N terminal on insert)
    • Xpress tag (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GCaMP3 cloned from G-CaMP3, Addgene plasmid 22692.
  • Terms and Licenses

Depositor Comments

The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915).

This destination vector was subsequently used to generate MAP2-GCaMP3 (GCaMP3 cloned from G-CaMP3, Addgene plasmid 22692).

The V5 and His tags present in the vector backbone are not expressed with the GCaMP3 insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAP2-GCaMP3 was a gift from John Gearhart (Addgene plasmid # 43917 ; http://n2t.net/addgene:43917 ; RRID:Addgene_43917)
  • For your References section:

    Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD. PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9. 10.1371/journal.pone.0028719 PubMed 22174877