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(Plasmid #68110)


Item Catalog # Description Quantity Price (USD)
Plasmid 68110 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    PhiC31 int/attP replaced with phiBT1 int/attP. aac3(IV) gene mostly deleted and hyg gene inserted.
  • Vector type
    Streptomyces integrating vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    apramycin resistance (fragment)
  • Species
    E. coli
  • Insert Size (bp)
  • Mutation

Gene/Insert 2

  • Gene/Insert name
  • Alt name
    Hygromycin resistance
  • Insert Size (bp)

Gene/Insert 3

  • Gene/Insert name
  • Alt name
    origin of transfer
  • Species
    E. coli
  • Insert Size (bp)

Gene/Insert 4

  • Gene/Insert name
  • Alt name
    phage attachment site
  • Species
    bacteriophage phiBT1
  • Insert Size (bp)

Gene/Insert 5

  • Gene/Insert name
  • Alt name
    phiBT1 Integrase
  • Species
    bacteriophage phiBT1
  • Insert Size (bp)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For research using this plasmid, please acknowledge the depositing laboratory and associated citation.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS82 was a gift from Maggie Smith (Addgene plasmid # 68110 ; ; RRID:Addgene_68110)
  • For your References section:

    Integration site for Streptomyces phage phiBT1 and development of site-specific integrating vectors. Gregory MA, Till R, Smith MC. J Bacteriol. 2003 Sep;185(17):5320-3. PubMed 12923110