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169,859 results

Showing: 181 - 200 of 169859 results

  6. gGBEv6.3-SpG

    Plasmid
    #202630
    Purpose
    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.
    Insert
    nSpG(D10A)-MPGv6.3
    Expression
    Mammalian
    Mutation
    SpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…
    Promoter
    hU6, EF1a, CBh
    Available Since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    202630-plasmid-map-sequence-id-398429

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  9. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid
    #189752
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189752-plasmid-map-sequence-id-375290

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  10. pLX301

    Plasmid
    #25895
    Purpose
    3rd generation lentiviral Gateway destination vector. Puromycin selection.
    Depositor
    David Root
    Article
    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Expression
    Mammalian
    Available Since
    July 30, 2010
    Availability
    Academic Institutions and Nonprofits only
    25895-plasmid-map-sequence-id-241925

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  13. pCALNL-GFP

    Plasmid
    #13770
    Purpose
    Cre recombinase-dependent expression of GFP, i.e. GFP is only expressed in the presence of Cre due to a transcriptional stop in between the loxP sites.
    Depositor
    Connie Cepko
    Article
    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10
    Insert
    EGFP
    Use
    Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 20, 2007
    Availability
    Academic Institutions and Nonprofits only
    13770-plasmid-map-sequence-id-7759

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  15. pAAV-hSyn-hM4D(Gi)-mCherry

    Plasmid
    #50475
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of human synapsin promoter.
    Depositor
    Bryan Roth
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50475-plasmid-map-sequence-id-171072

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Showing: 181 - 200 of 169859 results