161,202 results

1. PX458-AAVS1

   Plasmid

   #113194

   Purpose

   Encodes gRNA for human AAVS1

   Depositor

   Adam Karpf

   Insert

   AAVS1 (PPP1R12C, AAVS1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRH-1187 VSVGmut

   Plasmid

   #201913

   Purpose

   Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   VSV-G (K47Q, R354A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q, R354A

   Promoter

   CAG

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-KP1

   Plasmid

   #206504

   Purpose

   AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

   Depositor

   Mark Kay

   Article

   Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

   Insert

   KP1 capsid
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   p5

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOG44_FLP_recombinase (pEHA1338)

   Plasmid

   #209087

   Purpose

   FLP recombinase for FRT-mediated integration

   Depositor

   Vikram Khurana

   Article

   Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

   Insert

   FLP recombinase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV G-CEPIA1er

   Plasmid

   #58215

   Purpose

   Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   G-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

   Plasmid

   #118154

   Purpose

   Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-P2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   EF1a core and U6

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSNAP-tag (m) Vector

   Plasmid

   #101135

   Purpose

   Expression of protein of interest with SNAP-Tag in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Type

   Empty backbone

   Use

   Tags

   SNAP-tag (SNAP26m)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. PB-TRE-dCas9-KRAB-MeCP2

   Plasmid

   #122267

   Purpose

   SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   dCas9-KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFP-BAF

   Plasmid

   #101772

   Purpose

   Expresses EGFP tagged BAF in human cells

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. cyto-Ruby3-iATPSnFR1.0

    Plasmid

    #102551

    Purpose

    expresses cytoplasmic ATP sensor in mammalian cells with red reference protein

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    Ruby3-iATPSnFR1.0
    

    Use

    Tags

    Ruby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. cyto-iATPSnFR1.0

    Plasmid

    #102550

    Purpose

    cytoplasmic ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-Puro-AAVR-FLAG

    Plasmid

    #166716

    Purpose

    Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

    Depositor

    Jan Carette

    Article

    Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

    Insert

    AAVR-FLAG (KIAA0319L Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. HRV-B3 IRES

    Plasmid

    #189969

    Purpose

    Part 2 plasmid to introduce the HRV-B3 IRES into circRNAs

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    HRV-B3 IRES
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLCK-mScarlet3_C1

    Plasmid

    #189771

    Purpose

    Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LCK-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR CD19 tandem CAR

    Plasmid

    #113019

    Purpose

    for lentivirus production of CD19 tandem CAR

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P tandem FcR gamma intracellular and PI3k recruiting domain from CD19 (CD19 Human, Synthetic, Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3xnls-mScarlet3_N1

    Plasmid

    #189775

    Purpose

    Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    3xnls-mScarlet3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Cre

    Plasmid

    #198281

    Purpose

    Expresses Cre in mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid

    #122650

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    PylRS-AF, Pyl-tRNACUA
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDL278_P23-DsRed-Express2

    Plasmid

    #121505

    Purpose

    E. coli-streptococcal shuttle vector that expresses rfp

    Depositor

    Robert Shields

    Article

    Shields et al Appl Environ Microbiol. 2019 May 17. pii

    Insert

    DsRed-Express2
    

    Use

    E. coli streptococcal shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    P23

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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