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pRRLSIN.cPPT.PGK-GFP.WPRE
(Plasmid #12252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12252 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRRL
  • Backbone manufacturer
    Naldini lab
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    pRRL came from Naldini lab (JVI 98). R. Zufferey from Trono lab inserted the SIN in 98 and then WPRE in 99. Zennou et al finally inserted the cPPT.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit the Trono lab website http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRLSIN.cPPT.PGK-GFP.WPRE was a gift from Didier Trono (Addgene plasmid # 12252 ; http://n2t.net/addgene:12252 ; RRID:Addgene_12252)
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