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Addgene

pRRLSIN.cPPT.PGK-GFP.WPRE Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder and a digestion of the plasmid 12252 with EcoRV-HF, EcoRI-HF, and SpeI.

Our digest results confirm the expected banding patterns:

EcoRV-HF single digest: 1 band at 7.3

EcoRI-HF single digest: 2 bands - 1.9 and 6.4

SpeI single digest: 1 band at 7.3

Note: Full sequence for this plasmid is approximated.