-
Plasmid#188172PurposeMammalian Expression Plasmid of anti-VGAT (Mouse). Derived from hybridoma L118/14DepositorInsertanti-VGAT (Mus musculus) recombinant mouse monoclonal antibody (Slc32a1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mGeos-M-ALK1-N-13
Plasmid#57525PurposeLocalization: Membrane Receptor, Excitation: 503 / 569, Emission: 514 / 581DepositorInsertALK1 (ACVRL1 Human)
UseTagsmGeos-MExpressionMammalianMutationV501GPromoterCMVAvailable sinceJan. 16, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXR003: CasRx gRNA cloning backbone
Plasmid#109053PurposehU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863APromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-WT
Plasmid#17608PurposeMammalian expression of HA tagged ubiquitinDepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMitoTimer
Plasmid#52659PurposeExpresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivoDepositorInsertDsRed1-E5
UseDsredTagshuman cytochrome c oxidase subunit VIII mitochond…ExpressionMutationPromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-multi-CRISPR
Plasmid#85402PurposeLentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expressionDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dR8.2 dvpr
Plasmid#8455Purpose2nd generation lentiviral packaging plasmid. Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
EF.PGK.GFP
Plasmid#17618DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMXs-3XHA-EGFP-OMP25
Plasmid#83356PurposeFor tagging mitochondria with HA epitopes (HA-MITO construct)DepositorInsert3XHA-EGFP-OMP25
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
LCC-ICCG_pET21b
Plasmid#215408PurposeExpression construct for LCC-ICCG gene, codon optimized for expression in E. coliDepositorInsertLCC-ICCG
UseTagsExpressionBacterialMutationF243I/D238C/S283C/Y127GPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianMutationPromotertet-onAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianMutationPromotertet-onAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV.CAP-B10
Plasmid#175004Purposenon-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation systemDepositorInsertSynthetic construct isolate AAV.CAP-B10 VP1 gene
UseAAVTagsExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1…Promoterp41Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-mVenus-p27K−
Plasmid#176651PurposeIt encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).DepositorInsertmVenus-p27K- (Cdkn1b Mouse)
UseLentiviralTagsmVenusExpressionMutationMutant K- (lacks affinity to Cdk)PromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Tmem192-3xHA
Plasmid#102930PurposeExpress a lysosomal TagDepositorInsertTmem192 (TMEM192 Human)
UseLentiviralTagsHAExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV(CAT)T7-SB100
Plasmid#34879DepositorInsertSB100X transposase
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3_C1
Plasmid#189753PurposeC1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent proteinDepositorInsertmScarlet3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lamp1-RFP
Plasmid#1817PurposeMammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.DepositorInsertlysosome associated membrane protein 1 (Lamp1 Rat)
UseTagsRFPExpressionMammalianMutationD50E (not important for function of plasmid)PromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_Gal4VP64
Plasmid#79125PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only