172,433 results

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CROPseq-puro-v2

Plasmid

#127458

Purpose

Entry plasmid for guide cloning

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pEG BacMam

Plasmid

#160451

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ERT2CreERT2

Plasmid

#13777

Purpose

Mammalian expression of conditionally active form of Cre recombinase (activated in response to tamoxifen)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

ERT2-Cre-ERT2 fusion protein

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-IGHG1

Plasmid

#80795

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pXPR_050

Plasmid

#96925

Purpose

lentiviral expression of gRNA scaffold

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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V5-TurboID-NES_pCDNA3

Plasmid

#107169

Purpose

expresses V5-tagged TurboID in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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AbVec1.1-IGKC

Plasmid

#80796

Purpose

Expression of immunoglobulin light chains in mammalian cells, human kappa constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-VPR-2AmScarlet

Plasmid

#121964

Purpose

Mammalian expression, Transcription regulation module

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

VP64-p65-Rta fusion module fused with MCP, recruited by MS2 hairpin

Expression

Mammalian

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FokI-dCas9

Plasmid

#52970

Purpose

Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

Depositor

David Liu

Article

Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

Insert

Fok1 fused to dCas9

Use

CRISPR

Tags

Fok1 nuclease (homodimeric wildtype) and NLS

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-mVenus-p27K−

Plasmid

#176651

Purpose

It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

Depositor

Mohamed Bentires-Alj

Article

Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

Insert

mVenus-p27K- (Cdkn1b Mouse)

Use

Lentiviral

Tags

mVenus

Mutation

Mutant K- (lacks affinity to Cdk)

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHDM-Hgpm2

Plasmid

#164441

Purpose

Encodes codon-optimized HIV gagpol for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

Codon Optimized HIV-1 GagPol

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRC-CMV-Rev1b

Plasmid

#164443

Purpose

Encodes HIV-1 Rev accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Rev

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHDM-VSV-G

Plasmid

#164440

Purpose

Encodes VSV-G protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

VSV-G

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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SGEP

Plasmid

#111170

Purpose

miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

SFFV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Cre

Plasmid

#123133

Purpose

Mammalian expression vector for wild-type Cre.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Cre recombinase

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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AbVec1.1-IGLC2-XhoI

Plasmid

#99575

Purpose

Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1-Cre-PGK-Puro

Plasmid

#108543

Purpose

Lentivirus vector expessing Cre under EF1a promoter and puromycin under PGK promoter

Depositor

Javier Alcudia

Inserts

cre recombinase
Puromycin

Promoter

EF1apha and PGK

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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RG6

Plasmid

#80167

Purpose

fluorescent protein splicing reporter

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Expression

Mammalian

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_miRFP670

Plasmid

#113726

Purpose

Expresses the fluorescent protein miRFP670 in a lentiviral backbone

Depositor

Vanessa LaPointe

Insert

miRFP670

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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