165,307 results

1. pLX307 P2A GFP Luciferase

   Plasmid

   #193674

   Purpose

   Constitutive lentiviral expression of Luciferase

   Depositor

   William Hahn

   Article

   Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBP-LbCas12a

   Plasmid

   #113431

   Purpose

   Expresses 10xHis-MBP fused to LbCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Has Service

   Cloning Grade DNA

   Insert

   LbCas12a
   

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD33.1

   Plasmid

   #36267

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   PBAD

   Available Since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Eef1a-1955/-1>nls::Cas9::nls

   Plasmid

   #59987

   Purpose

   Eef1a (EF1alpha) promoter driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Eef1a (EF1alpha) -1955/-1

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKm-HCBE

   Plasmid

   #170333

   Purpose

   a modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium meliloti

   Depositor

   Deqiang Duanmu

   Article

   Wang et al Front Microbiol. 2021 Jun 18;12:686008.

   Insert

   Rhizobium codon-optimized CBEs
   

   Use

   CRISPR

   Mutation

   Cas9n-D10A

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. plentiCMV-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196511

   Purpose

   Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS1C

   Plasmid

   #55168

   Purpose

   Empty vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Available Since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMIG-FLAG-MLL-AF9

   Plasmid

   #71443

   Purpose

   Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

   Depositor

   Daisuke Nakada

   Article

   Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

   Insert

   MLL-Af9 (KMT2A Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-PspCas13b-gRNA-Actb1216

   Plasmid

   #155368

   Purpose

   PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   PspCas13b gRNA targeting Actb 1216
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458-AAVS1

    Plasmid

    #113194

    Purpose

    Encodes gRNA for human AAVS1

    Depositor

    Adam Karpf

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-2xFYVE

    Plasmid

    #140047

    Purpose

    Expresses GFP-tagged HRS 2xFYVE in mammalian cells

    Depositor

    Harald Stenmark

    Article

    Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

    Insert

    tandem HRS FYVE domain (Hgs Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    two FYVE domains (amino acids 147-223), linked by…

    Promoter

    CMV

    Available Since

    May 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pink Flamindo

    Plasmid

    #102356

    Purpose

    biosensor for cAMP in mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

    Insert

    Pink Flamindo
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-XPO1

    Plasmid

    #116804

    Purpose

    Lentiviral expression of XPO1

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.1 hSCN5A

    Plasmid

    #145374

    Purpose

    expresses human Nav1.5 (WT) in mammalian cells

    Depositor

    Hugues Abriel, Robert Kass

    Article

    Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1_jRCaMP1b

    Plasmid

    #183758

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    jRCaMP1b
    

    Tags

    NES

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-p62

    Plasmid

    #28027

    Purpose

    Mammalian expression of HA tagged human p62

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    p62 (SQSTM1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSGDlucV3.0

    Plasmid

    #119760

    Purpose

    dual luciferase with FMDV StopGo sequences flanking MCS

    Depositor

    John Atkins

    Article

    Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40 and T7

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV2/11

    Plasmid

    #240486

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid protein

    Depositor

    Addgene Research Program, Meghan Rego

    Insert

    AAV2 Rep and AAV11 Cap
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    July 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. T444T

    Plasmid

    #113081

    Purpose

    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-dCas13-M3nls

    Plasmid

    #155366

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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