177,069 results
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Plasmid#62226PurposeConstitutive expression of sgRNA without donor editing template DNADepositorInsertsgRNA
UseCRISPRExpressionBacterialPromoterpij23119Available sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Flag mTOR wt
Plasmid#26603PurposeMammalian expression of human flag-tagged mTorDepositorAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SBE4-Luc
Plasmid#16495PurposeLuciferase reporter containing four copies of the Smad binding siteDepositorPublicationInsertSmad binding element
UseLuciferaseTagsluciferaseExpressionMammalianAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable sinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-mRFP
Plasmid#13032PurposeMammalian expression vector for cloning your gene fused to mRFPDepositorInsertMonomer Red Fluorescent Protein
ExpressionMammalianAvailable sinceNov. 7, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVCT-tTR-KRAB
Plasmid#11643PurposeTet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-iCas9-neo
Plasmid#85400PurposeLentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequenceDepositorInsertFlag-iCas9-P2A-GFP
UseLentiviralTagsFlag-iCas9-P2A-GFPAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
psPAX2-D64V
Plasmid#63586PurposepsPAX2-D64V is derived from psPAX2 plasmid. It is a packaging plasmid for making Integrase deficient lentiviral vectors since has a point mutation in the integrase gene.DepositorInsertPackaging plasmid for integrase deficient lentiviral vectors
UseIntegrase deficient lentiviral packaging vectorAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available sinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21a-BirA
Plasmid#20857DepositorInsertBirA
Tags6xHisExpressionBacterialAvailable sinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRISPRoff-v2.1
Plasmid#167981PurposeExpresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoterDepositorInsertCRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
Tags2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFPExpressionMammalianPromoterCAGAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry2-N1
Plasmid#54517PurposeLocalization: N1 Cloning Vector, Excitation: 587, Emission: 610DepositorTypeEmpty backboneTagsmCherry2ExpressionMammalianPromoterCMVAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-LC3 (human)
Plasmid#24920PurposeMammalian expression of LC3 fused to EGFPDepositorAvailable sinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSP1E3D1
Plasmid#20066PurposeBacterial expression of an extended membrane scaffold protein (MSP1E3D1) for Nanodisc formationDepositorInsertMSP1E3D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeats of …Available sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMitoTimer
Plasmid#52659PurposeExpresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivoDepositorInsertDsRed1-E5
UseDsredTagshuman cytochrome c oxidase subunit VIII mitochond…PromoterCMVAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3-NFAT luciferase
Plasmid#17870Purpose3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissueDepositorInsert3x NFAT binding sequence (IL2 )
UseLuciferaseTagsIL2 promoter and LuciferaseExpressionMammalianPromoterIL-2 promoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pX333
Plasmid#64073PurposeVector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.DepositorTypeEmpty backboneTags3XFLAG-Cas9ExpressionMammalianPromoterCBh; U6Available sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by