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Lenti-iCas9-neo
(Plasmid #85400)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85400 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pInducer-20
  • Backbone size w/o insert (bp) 10000
  • Total vector size (bp) 15188
  • Vector type
    Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag-iCas9-P2A-GFP
  • Insert Size (bp)
    5000
  • Tag / Fusion Protein
    • Flag-iCas9-P2A-GFP

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-iCas9-neo was a gift from Qin Yan (Addgene plasmid # 85400)
  • For your References section:

    An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q. Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25. 10.1093/nar/gkw660 PubMed 27458201