Purpose(Empty Backbone) Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85402||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
|Cloning Grade DNA||85402-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$95|
This material is available to academics and nonprofits only.
- Backbone size (bp) 15069
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
Information for Cloning Grade DNA (Catalog # 85402-DNA.cg) ( Back to top )
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $95 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Terms and Licenses
- Not Available to Industry
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lenti-multi-CRISPR was a gift from Qin Yan (Addgene plasmid # 85402 ; http://n2t.net/addgene:85402 ; RRID:Addgene_85402)
For your References section:An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q. Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25. 10.1093/nar/gkw660 PubMed 27458201