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Sung-Hou Kim Lab Plasmids


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Title Authors Publication
Cloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A. Kim KK, Yokota H, Kim R, Kim SH Protein Sci. 1997 Oct . 6(10):2268-70.
Crystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 A resolution. Wang W, Kim R, Jancarik J, Yokota H, Kim SH Structure. 2001 Jan 10. 9(1):65-71.
Solution nuclear magnetic resonance structure of a protein disulfide oxidoreductase from Methanococcus jannaschii. Cave JW, Cho HS, Batchelder AM, Yokota H, Kim R, Wemmer DE Protein Sci. 2001 Feb . 10(2):384-96.
Crystal structure of conserved hypothetical protein Aq1575 from Aquifex aeolicus. Shin DH, Yokota H, Kim R, Kim SH Proc Natl Acad Sci U S A. 2002 Jun 11. 99(12):7980-5.
Crystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima. Shin DH, Roberts A, Jancarik J, Yokota H, Kim R, Wemmer DE, Kim SH Protein Sci. 2003 Jul . 12(7):1464-72.
Structural analyses of peptide release factor 1 from Thermotoga maritima reveal domain flexibility required for its interaction with the ribosome. Shin DH, Brandsen J, Jancarik J, Yokota H, Kim R, Kim SH J Mol Biol. 2004 Jul 30. 341(1):227-39.
Crystal structure of a stress inducible protein from Mycoplasma pneumoniae at 2.85 A resolution. Choi IG, Shin DH, Brandsen J, Jancarik J, Busso D, Yokota H, Kim R, Kim SH J Struct Funct Genomics. 2003 . 4(1):31-4.
Crystal structure of a hypothetical protein, TM841 of Thermotoga maritima, reveals its function as a fatty acid-binding protein. Schulze-Gahmen U, Pelaschier J, Yokota H, Kim R, Kim SH Proteins. 2003 Mar 1. 50(4):526-30.
Solution structure of a putative ribosome binding protein from Mycoplasma pneumoniae and comparison to a distant homolog. Rubin SM, Pelton JG, Yokota H, Kim R, Wemmer DE J Struct Funct Genomics. 2003 . 4(4):235-43.
Crystal structure of a protein associated with cell division from Mycoplasma pneumoniae (GI: 13508053): a novel fold with a conserved sequence motif. Chen S, Jancrick J, Yokota H, Kim R, Kim SH Proteins. 2004 Jun 1. 55(4):785-91.
Structure of OsmC from Escherichia coli: a salt-shock-induced protein. Shin DH, Choi IG, Busso D, Jancarik J, Yokota H, Kim R, Kim SH Acta Crystallogr D Biol Crystallogr. 2004 May . 60(Pt 5):903-11.
Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2 A resolution. Chen S, Shin DH, Pufan R, Kim R, Kim SH Proteins. 2004 Sep 1. 56(4):839-43.
Crystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution. Du X, Choi IG, Kim R, Wang W, Jancarik J, Yokota H, Kim SH Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14079-84.
Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii. Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim SH Biochemistry. 2001 Nov 27. 40(47):14166-72.
Crystal structure of ScpB from Chlorobium tepidum, a protein involved in chromosome partitioning. Kim JS, Shin DH, Pufan R, Huang C, Yokota H, Kim R, Kim SH Proteins. 2006 Feb 1. 62(2):322-8.
Crystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters. Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH J Biol Chem. 2005 Apr 22. 280(16):15960-6.
Crystal structure of DNA sequence specificity subunit of a type I restriction-modification enzyme and its functional implications. Kim JS, DeGiovanni A, Jancarik J, Adams PD, Yokota H, Kim R, Kim SH Proc Natl Acad Sci U S A. 2005 Mar 1. 102(9):3248-53.
Crystal structure of a heat-inducible transcriptional repressor HrcA from Thermotoga maritima: structural insight into DNA binding and dimerization. Liu J, Huang C, Shin DH, Yokota H, Jancarik J, Kim JS, Adams PD, Kim R, Kim SH J Mol Biol. 2005 Jul 29. 350(5):987-96.
Crystal structure of a phosphotransacetylase from Streptococcus pyogenes. Xu QS, Shin DH, Pufan R, Yokota H, Kim R, Kim SH Proteins. 2004 May 1. 55(2):479-81.
Crystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain. Shin DH, Lou Y, Jancarik J, Yokota H, Kim R, Kim SH Proc Natl Acad Sci U S A. 2004 Sep 7. 101(36):13198-203.
Crystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum. Shin DH, Oganesyan N, Jancarik J, Yokota H, Kim R, Kim SH J Biol Chem. 2005 May 6. 280(18):18326-35.
Structural characterization of an iron-sulfur cluster assembly protein IscU in a zinc-bound form. Liu J, Oganesyan N, Shin DH, Jancarik J, Yokota H, Kim R, Kim SH Proteins. 2005 Jun 1. 59(4):875-81.
Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate. Xu QS, Jancarik J, Lou Y, Kuznetsova K, Yakunin AF, Yokota H, Adams P, Kim R, Kim SH J Struct Funct Genomics. 2005 Nov 9. ():1-11.
A model for the determination of the 3D-spatial distribution of the functions of the hormone-binding domain of receptors that bind 3-keto-4-ene steroids. Lemesle-Varloot L, Ojasoo T, Mornon JP, Raynaud JP J Steroid Biochem Mol Biol. 1992 Mar . 41(3-8):369-88.
Crystal structure of tRNA (m1G37) methyltransferase from Aquifex aeolicus at 2.6 A resolution: a novel methyltransferase fold. Liu J, Wang W, Shin DH, Yokota H, Kim R, Kim SH Proteins. 2003 Nov 1. 53(2):326-8.
Crystal structure of TM1457 from Thermotoga maritima. Shin DH, Lou Y, Jancarik J, Yokota H, Kim R, Kim SH J Struct Biol. 2005 Nov . 152(2):113-7.
SH Kim lab plasmids (unpublished)
Overexpression of archaeal proteins in Escherichia coli Rosalind Kim, Steve J. Sandler, Stanley Goldman, Hisao Yokota, Alvin J. Clark and Sung-Hou Kim Biotechnology Letters. March 1998. 20(3): 207-210.
Purification, crystallization and preliminary X-ray crystallographic analysis of Pyrococcus furiosus DNA polymerase. Goldman S, Kim R, Hung LW, Jancarik J, Kim SH Acta Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8.
Crystal structure of a flavin-binding protein from Thermotoga maritima. Wang W, Kim R, Jancarik J, Yokota H, Kim SH Proteins. 2003 Sep 1. 52(4):633-5.
Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP. Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH J Mol Biol. 2005 Nov 25. 354(2):289-303.
Crystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain. Shin DH, Nguyen HH, Jancarik J, Yokota H, Kim R, Kim SH Biochemistry. 2003 Nov 25. 42(46):13429-37.
Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution. Wang H, Boisvert D, Kim KK, Kim R, Kim SH EMBO J. 2000 Feb 1. 19(3):317-23.
Crystal structure of MJ1247 protein from M. jannaschii at 2.0 A resolution infers a molecular function of 3-hexulose-6-phosphate isomerase. Martinez-Cruz LA, Dreyer MK, Boisvert DC, Yokota H, Martinez-Chantar ML, Kim R, Kim SH Structure. 2002 Feb . 10(2):195-204.
Structure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii. Huang L, Hung L, Odell M, Yokota H, Kim R, Kim SH J Struct Funct Genomics. 2002 . 2(3):121-7.
Crystal structure of archaeal RNase HII: a homologue of human major RNase H. Lai L, Yokota H, Hung LW, Kim R, Kim SH Structure. 2000 Aug 15. 8(8):897-904.
Purification, crystallization, and preliminary X-ray crystallographic data analysis of small heat shock protein homolog from Methanococcus jannaschii, a hyperthermophile. Kim KK, Yokota H, Santoso S, Lerner D, Kim R, Kim SH J Struct Biol. 1998 Jan . 121(1):76-80.
Structural and functional characterization of a novel phosphodiesterase from Methanococcus jannaschii. Chen S, Yakunin AF, Kuznetsova E, Busso D, Pufan R, Proudfoot M, Kim R, Kim SH J Biol Chem. 2004 Jul 23. 279(30):31854-62.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
11304pET21a.MJ0285small heat shock proteinBacterial ExpressionPurification, crystallization, and preliminary X-ray crystallographic data analysis of small heat shock protein homolog from Methanococcus jannaschii, a hyperthermophile. J Struct Biol. 1998 Jan . 121(1):76-80. pET21a MJ_0285 MJ_0285, MJ0285 Add to Cart
11410pET21a.MJ1228eukaryotic translation initiation factorBacterial ExpressionCloning, expression, and crystallization of a hyperthermophilic protein that is homologous to the eukaryotic translation initiation factor, eIF5A. Protein Sci. 1997 Oct . 6(10):2268-70. pET21a Add to Cart
11411pET21a.MJ0697fibrillarin homolog (Other)Bacterial ExpressionCrystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6 A resolution. EMBO J. 2000 Feb 1. 19(3):317-23. pET21a MJ_0697 MJ_0697, MJ0697 Add to Cart
11412pET21a.MJ0135RNase HII (Other)Bacterial ExpressionCrystal structure of archaeal RNase HII: a homologue of human major RNase H. Structure. 2000 Aug 15. 8(8):897-904. pET21a rnhB Add to Cart
11413pET21a.MJ1594phosphoserine phosphataseBacterial ExpressionCrystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 A resolution. Structure. 2001 Jan 10. 9(1):65-71. pET21a MJ_1594 MJ_1594, MJ1594 Add to Cart
11414pET21a.MJ12473-hexulose-6-phosphate isomerase (Other)Bacterial ExpressionCrystal structure of MJ1247 protein from M. jannaschii at 2.0 A resolution infers a molecular function of 3-hexulose-6-phosphate isomerase. Structure. 2002 Feb . 10(2):195-204. pET21a MJ_1247 MJ_1247, MJ1247 Add to Cart
11415pET21a.MJ0307disulfide oxidoreductaseBacterial ExpressionSolution nuclear magnetic resonance structure of a protein disulfide oxidoreductase from Methanococcus jannaschii. Protein Sci. 2001 Feb . 10(2):384-96. pET21a Add to Cart
11416pET21a.AQ_1575hypotheticalBacterial ExpressionCrystal structure of conserved hypothetical protein Aq1575 from Aquifex aeolicus. Proc Natl Acad Sci U S A. 2002 Jun 11. 99(12):7980-5. pET21a aq_1575 Add to Cart
11417pET21a.MJ0882methyltransferase (Other)Bacterial ExpressionStructure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii. J Struct Funct Genomics. 2002 . 2(3):121-7. pET21a MJ_0882 Add to Cart
11418pET21a.TM0651phosphataseBacterial ExpressionCrystal structure of a phosphatase with a unique substrate binding domain from Thermotoga maritima. Protein Sci. 2003 Jul . 12(7):1464-72. pET21a Add to Cart
11419pET21a.TM0857flavin binding protein (Other)Bacterial ExpressionCrystal structure of a flavin-binding protein from Thermotoga maritima. Proteins. 2003 Sep 1. 52(4):633-5. pET21a TM0857 TM0857 Add to Cart
11420pET21a.TM1363peptide release factor IBacterial ExpressionStructural analyses of peptide release factor 1 from Thermotoga maritima reveal domain flexibility required for its interaction with the ribosome. J Mol Biol. 2004 Jul 30. 341(1):227-39. pET21a prfA Add to Cart
11421pSKB3.TM1777NusA (Other)Bacterial ExpressionCrystal structure of NusA from Thermotoga maritima and functional implication of the N-terminal domain. Biochemistry. 2003 Nov 25. 42(46):13429-37. pSKB3 nusA Add to Cart
11430pSKB3.MPN625peroxiredoxinBacterial ExpressionCrystal structure of a stress inducible protein from Mycoplasma pneumoniae at 2.85 A resolution. J Struct Funct Genomics. 2003 . 4(1):31-4. pSKB3 MPN625 C12_orf141 Add to Cart
11431pSKB3.TM1468fatty acid binding proteinBacterial ExpressionCrystal structure of a hypothetical protein, TM841 of Thermotoga maritima, reveals its function as a fatty acid-binding protein. Proteins. 2003 Mar 1. 50(4):526-30. pSKB3 Add to Cart
11432pSKB3.MPN156ribosome binding proteinBacterial ExpressionSolution structure of a putative ribosome binding protein from Mycoplasma pneumoniae and comparison to a distant homolog. J Struct Funct Genomics. 2003 . 4(4):235-43. pSKB3 Add to Cart
11433pSKB3.MPN314cell division proteinBacterial ExpressionCrystal structure of a protein associated with cell division from Mycoplasma pneumoniae (GI: 13508053): a novel fold with a conserved sequence motif. Proteins. 2004 Jun 1. 55(4):785-91. pSKB3 Add to Cart
11436pSKB3.EG10680OsmCBacterial ExpressionStructure of OsmC from Escherichia coli: a salt-shock-induced protein. Acta Crystallogr D Biol Crystallogr. 2004 May . 60(Pt 5):903-11. pSKB3 osmC ECK1476, JW1477 Add to Cart
11437pSKB3.MPN348methenyltetrahydrofolate synthetaseBacterial ExpressionCrystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2 A resolution. Proteins. 2004 Sep 1. 56(4):839-43. pSKB3 Add to Cart
11452pHMMa.PH1704intracellular proteaseBacterial ExpressionCrystal structure of an intracellular protease from Pyrococcus horikoshii at 2-A resolution. Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14079-84. pHMMa Add to Cart
11453pHMMa.PH0999pyrazinamidaseBacterial ExpressionCrystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii. Biochemistry. 2001 Nov 27. 40(47):14166-72. pHMMa PH0999 Add to Cart
11526pLIC.B3.CT0478ScpBBacterial ExpressionCrystal structure of ScpB from Chlorobium tepidum, a protein involved in chromosome partitioning. Proteins. 2006 Feb 1. 62(2):322-8. pLIC.B3 Add to Cart
11527pLIC.B3.TM1734PhoUBacterial ExpressionCrystal structure of a PhoU protein homologue: a new class of metalloprotein containing multinuclear iron clusters. J Biol Chem. 2005 Apr 22. 280(16):15960-6. pLIC.B3 TM1734 TM1734 Add to Cart
11528pLIC.B3.MJ0130mType I restriction-modification enzymeBacterial ExpressionCrystal structure of DNA sequence specificity subunit of a type I restriction-modification enzyme and its functional implications. Proc Natl Acad Sci U S A. 2005 Mar 1. 102(9):3248-53. pLIC.B3 MJ_0130m Add to Cart
11529pLIC.B3.AF2373NAD kinaseBacterial ExpressionCrystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP. J Mol Biol. 2005 Nov 25. 354(2):289-303. pLIC.B3 AF2373 Add to Cart
11530pLIC.B3.TM0851heat-inducible transcriptional repressorBacterial ExpressionCrystal structure of a heat-inducible transcriptional repressor HrcA from Thermotoga maritima: structural insight into DNA binding and dimerization. J Mol Biol. 2005 Jul 29. 350(5):987-96. pLIC.B3 TM0851 Add to Cart
11531pLIC.B4.SPy1128phosphotransacetylaseBacterial ExpressionCrystal structure of a phosphotransacetylase from Streptococcus pyogenes. Proteins. 2004 May 1. 55(2):479-81. pLIC.B4 Add to Cart
11532pLIC.B4.TM1717YjeQ GDP binding proteinBacterial ExpressionCrystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain. Proc Natl Acad Sci U S A. 2004 Sep 7. 101(36):13198-203. pLIC.B4 TM1717 Add to Cart
11533pLIC.B4.Ta1145nicotinate phosphoribosyltransferaseBacterial ExpressionCrystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum. J Biol Chem. 2005 May 6. 280(18):18326-35. pLIC.B4 Ta1145 Add to Cart
11534pLIC.B4.SPy0289IscUBacterial ExpressionStructural characterization of an iron-sulfur cluster assembly protein IscU in a zinc-bound form. Proteins. 2005 Jun 1. 59(4):875-81. pLIC.B4 Add to Cart
11535pLIC.B4.BSU37660phosphotransacetylaseBacterial ExpressionCrystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate. J Struct Funct Genomics. 2005 Nov 9. ():1-11. pLIC.B4 Add to Cart
11536pLIC.B2.MPN330FAD synthetaseBacterial ExpressionA model for the determination of the 3D-spatial distribution of the functions of the hormone-binding domain of receptors that bind 3-keto-4-ene steroids. J Steroid Biochem Mol Biol. 1992 Mar . 41(3-8):369-88. pLIC.B2 Add to Cart
11538pLIC.B2.MJ0936phosphodiesteraseBacterial ExpressionStructural and functional characterization of a novel phosphodiesterase from Methanococcus jannaschii. J Biol Chem. 2004 Jul 23. 279(30):31854-62. pLIC.B2 Add to Cart
11539pHM6g.AQ_1489tRNA methyltransferaseBacterial ExpressionCrystal structure of tRNA (m1G37) methyltransferase from Aquifex aeolicus at 2.6 A resolution: a novel methyltransferase fold. Proteins. 2003 Nov 1. 53(2):326-8. pHM6g Add to Cart
11540pHM6g.TM1457TM1457Bacterial ExpressionCrystal structure of TM1457 from Thermotoga maritima. J Struct Biol. 2005 Nov . 152(2):113-7. pHM6g TM1457 Add to Cart
12218pRG-12pRas189 Gly12Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12219pRV-12pRas189 Val12Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12220pRas171 Trp40pras171 Trp40Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12221pRas171 Ala12pras171 Ala12Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12222pRGH-12pRas189 Gly12 truncatedBacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12223pRVH-12pRas171 Val12 truncatedBacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12224pRas171 Ser13pras171 Ser13Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12225pRV-12 166aapRas166 Val12 truncatedBacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12226pRas171 Val13pras171 Val13Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12227pRGW40pRas189 W40Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12228pRas171 Pro12pras171 Pro12Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12229pRas171 Thr13pras171 Thr13Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12230pR Leu61pRas189 Leu61Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12231pRL-12pRas189 L12Bacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12232pRLH-12pRas189 Leu12 truncatedBacterial Expression SH Kim lab plasmids (unpublished) pCT-1 Add to Cart
12234pSJS 1240Bacterial ExpressionOverexpression of archaeal proteins in Escherichia coli Biotechnology Letters. March 1998. 20(3): 207-210. pSJS1240 Add to Cart
12509pET16B.PfuPfu DNA polymerase (Other)Bacterial ExpressionPurification, crystallization and preliminary X-ray crystallographic analysis of Pyrococcus furiosus DNA polymerase. Acta Crystallogr D Biol Crystallogr. 1998 Sep 1. 54(Pt 5):986-8. Bacterial expression of Pfu DNA polymerase pET16b Add to Cart