172,049 results

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prhaBAD-anti-rabbit-Nb-Hia5

Plasmid

#239627

Purpose

Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Sidhwani et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Rabbit Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET-16b-10His-TEV-MCES_VACCA_D1_D12

Plasmid

#203671

Purpose

The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

Depositor

Stephen Cusack

Article

Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

Insert

MCES_VACCA_D1_D12

Tags

10xHis

Expression

Bacterial

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDSG289

Plasmid

#115608

Purpose

surface-displayed Nb2, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG291

Plasmid

#115605

Purpose

surface-displayed Null, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDSG287

Plasmid

#115606

Purpose

surface-displayed Ag2, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry-p23

Plasmid

#108224

Purpose

Mammalian expression vector for mCherry fusion to human p23

Depositor

Didier Picard

Article

Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

Insert

mCherry-p23 (PTGES3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-ATLASsnCre

Plasmid

#232351

Purpose

Anterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.

Depositor

Don Arnold

Article

Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

Has Service

AAV5

Insert

ATLASsnCre

Use

AAV

Tags

ALFA-Tag

Promoter

hSyn

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 myc CIITA (P#808)

Plasmid

#14650

Depositor

Matija Peterlin

Article

Kanazawa et al Immunity. 2000 Jan . 12(1):61-70.

Insert

CIITA (CIITA Human)

Tags

Myc

Expression

Mammalian

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV/SB11

Plasmid

#26552

Depositor

Perry Hackett

Article

Ivics et al Cell. 1997 Nov 14. 91(4):501-10.

Insert

Sleeping Beauty

Expression

Bacterial

Mutation

Methionine 243 to Glutamine Threonine 136 to Arg…

Available Since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-CuO-KRAB-dCas9-BFP-CymR-Puro

Plasmid

#248161

Purpose

Expresses KRAB fused to dCas9 and BFP upon cumate induction

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Inserts

KRAB-dCas9-HA-tagBFP
HA-CymR-T2A-puro

Use

CRISPR

Tags

HA and tagBFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pBOB-Adcy3-3xFlag

Plasmid

#245989

Purpose

Express 3xFlag C-terminal tagged wild-type mouse ADCY3 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

Insert

Adcy3 (Adcy3 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-VPR

Plasmid

#63800

Purpose

SP-dCas9-VPR with doxycycline-inducible expression

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

VPR (VP64-p65-Rta)

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold

Plasmid

#212017

Purpose

mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.22-PGK1-HRE::dLUC

Plasmid

#128095

Purpose

Hypoxia response element real-time luciferase reporter

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)

Use

Luciferase

Tags

drives expression of destabilized luciferase (2CP…

Expression

Mammalian

Promoter

inserted PGK1 hypoxia response elements (HRE) (x3)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pOpen-T4 PNK

Plasmid

#165520

Purpose

The most commonly used polynucleotide kinase.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T4 PNK

Use

Synthetic Biology

Available Since

Aug. 3, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L4552 x6NFATRE (NFAT) Ben DsRed-Express2 in TUPV1

Plasmid

#244201

Purpose

Expression of a DsRed-Express2 reporter under an NFAT-responsive promoter

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

NFAT-responsive promoter (6 NFAT binding sites + YB_TATA), dsRed-Express2 reporter

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-SCRAMBLE

Plasmid

#26701

Purpose

3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selection

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

scramble

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-L

Plasmid

#59025

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-L

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-TALEN-R

Plasmid

#59026

Purpose

TALEN designed to target the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

AAVS1-TALEN-R

Use

AAV and TALEN

Tags

3xFlag and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CRE-CytoTape-V5

Plasmid

#239430

Purpose

CytoTape signal monomer for recording phosphorylated CREB protein activity

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

CRE promoter

Available Since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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