166,332 results

1. pH6HTC_PRMT1-Halo

   Plasmid

   #175334

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PRMT1 (PRMT1 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ABE8e

   Plasmid

   #138489

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9
   

   Expression

   Mammalian

   Available Since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFast-hHus1

   Plasmid

   #222469

   Purpose

   Expresses hHus1 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hHus1 (HUS1 Human)
   

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. VPS13D^mScarlet

   Plasmid

   #174110

   Purpose

   The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

   Depositor

   Pietro De Camilli

   Article

   Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   Codon optimized VPS13D
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   With an internal mScarlet tag in aminoacid positi…

   Promoter

   CMV

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFast-Flag-hRad9

   Plasmid

   #222468

   Purpose

   Expresses hRad9 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad9 (RAD9A Human)
   

   Tags

   Flag epitope

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFast-hRad1

   Plasmid

   #222471

   Purpose

   Expresses hRad1 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad1 (RAD1 Human)
   

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFast-His6-hRad17

   Plasmid

   #222465

   Purpose

   Expresses hRad17 in insect cells

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

   Insert

   hRad17 (RAD17 Human)
   

   Tags

   His

   Expression

   Insect

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIVT_hifiDn29-dCas9-P2A-GFP

   Plasmid

   #247168

   Purpose

   In vitro transcription of human codon optimized fusion of hifiDn29-dCas9-P2A-GFP

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   hifiDn29-dCas9-P2A-GFP
   

   Use

   In vitro transcription

   Tags

   SV40 NLS

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

   Promoter

   mutated T7

   Available Since

   Nov. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVone-AAV9-CMV-eGFP

   Plasmid

   #230928

   Purpose

   pAAVone plasmid is used to package AAV-CMV-eGFP with an AAV9 serotype, utilizing the AAVone single-plasmid AAV production system.

   Depositor

   Qizhao Wang

   Article

   Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

   Insert

   eGFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV2/5

    Plasmid

    #232922

    Purpose

    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Rep2/Cap5
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Sept. 17, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. AiP15072 - pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA (Alias: CN5072)

    Plasmid

    #230402

    Purpose

    AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    iCre(R297T)
    

    Use

    AAV

    Promoter

    minBG

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTFP1-N1

    Plasmid

    #54521

    Purpose

    Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Tags

    mTFP1

    Expression

    Mammalian

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-IQGAP1

    Plasmid

    #30112

    Depositor

    David Sacks

    Article

    Ren et al J Biol Chem. 2005 Oct 14. 280(41):34548-

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Tags

    tandem EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. ppyCAG_RNaseH1_WT

    Plasmid

    #111906

    Purpose

    Express RNASEH1 in mammalian cell.

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hIBA1a-GFP-miR124T

    Plasmid

    #214147

    Purpose

    AAV vector to restrict GFP expression in microglia.

    Depositor

    Chun-Li Zhang

    Article

    Serrano et al iScience. 2024 Aug 12;27(9):110706. doi:

    Has Service

    AAV5

    Insert

    GFP
    

    Use

    AAV

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Gregg Beckham, Christopher Johnson

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-29b(+)_NSP4ss_SerM_NucA-His

    Plasmid

    #218190

    Purpose

    Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Tags

    His and NSP4 signal secretion peptide

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-LgBit

    Plasmid

    #234738

    Purpose

    Mammalian expression of large NanoBit fragment (LgBit)

    Depositor

    Nevin Lambert

    Article

    Jang et al Elife. 2024 Nov 8;13:RP97033. doi: 10.75

    Insert

    LgBit
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-rGBD

    Plasmid

    #26732

    Depositor

    William Bement

    Article

    Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

    Insert

    rhotekin (rtkn2 Mouse)
    

    Use

    Reporter for rho

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    RhoA binding domain of rhotekin

    Available Since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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