171,443 results
-
Plasmid#118281PurposeExpresses GFPmut2 with low cell-to-cell variation and lactose inductionDepositorInsertGFPmut2
ExpressionBacterialPromoterpLlacO-1Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL3402 (pTnsABf_Pse)
Plasmid#200901PurposeExpresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.DepositorInsertPseTnsA-TnsB
TagsInternal NLS between TnsA-TnsB fusionExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL5069 (pDonor(AAVS1),CRISPR_Pse)
Plasmid#200904PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a splice acceptor-T2A-PuroR-T2A-eGFP cargo, and a CRISPR RNA under a U6 promoter. Total transposon size = 2,024 bp.DepositorInsertMini Tn7016 (AAVS1 NGS), PseCRISPR (tSL425)
ExpressionMammalianAvailable SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/Rabaptin-5:R5BD
Plasmid#168808PurposeProduction of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily membersDepositorInsertRab5-binding domain (aa739-862) of Rabaptin-5
TagsGSTExpressionBacterialAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-14-3-3 epsilon
Plasmid#13273DepositorAvailable SinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLSL
Plasmid#51493PurposeT-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.DepositorTypeEmpty backboneUsePlant t-dna plasmidPromotervirion-sense LIR and 2x35SAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT2-mApple
Plasmid#180319Purposemammalian expression of human SEPT2 fused to monomeric AppleDepositorAvailable SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP
Plasmid#214679PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Hygro-GFP
Plasmid#61215PurposeRetrovirus expressing GFPDepositorInsertGFP
UseRetroviralAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
Plasmid#115694PurposeAll-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.DepositorInsertsNmeCas9
Nme-sgRNA
UseCRISPRTagsNLS and NLS, HAExpressionInsect and MammalianMutationNone and fusion of repeat/tracrRNA to make a sgRNAPromoterEF-1 alpha and U6Available SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY027
Plasmid#84742PurposeExpresses huLbCpf1 and crRNA guideDepositorInserthuLbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
PRS_8-Jaws-tdTomato
Plasmid#192593PurposeAAV-vector for optogenetic silencing of noradrenergic neuronsDepositorInsertJaws
UseAAVTagstdTomato-ER2ExpressionMammalianPromoterPRSX8Available SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
tph-1(short)::rpl-22-HA
Plasmid#122203PurposeC. elegans tagged ribosome expression plasmidDepositorInserttph-1(short)::rpl-22-HA
Available SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30-2-GPD1L
Plasmid#83912Purposestable overexpressionDepositorInsertglycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
Tags6x His tagsExpressionBacterialPromoterT7Available SinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
1552 pcDNA3 HA gamma adaptin 1
Plasmid#10712DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHK316
Plasmid#235474PurposeInducible gene VIII by Ptet promoterDepositorInsertphage M13 geneVIII
ExpressionBacterialAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_01
Plasmid#194662PurposeProtein production of the split HaloTag based calcium recorder Caprola_01 in bacteriaDepositorInsertCaprola_01
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK234
Plasmid#73324PurposeProduces Janthinobacterium lividum violacein biosynthesis proteins VioA, VioB, and VioCDepositorInsertsviolacein biosynthesis protein VioA
violacein biosynthesis protein VioB
violacein biosynthesis protein VioC
ExpressionBacterialPromoterT7 and n/aAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only