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Showing: 121 - 140 of 172500 results

  5. pETDuet_huCAD_ICADL (TevS)

    Plasmid
    #100098
    Purpose
    dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences
    Depositor
    Jim Wells
    Article
    Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112
    Insert
    DNA fragmentation factor subunit alpha (CAD, DFFA Human)
    Expression
    Bacterial
    Mutation
    two caspase cleavage sites (DETD-117 and DAVD-224…
    Promoter
    T7
    Available Since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    100098-plasmid-map-sequence-id-261413

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  9. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid
    #73497
    Purpose
    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker
    Depositor
    Bruce Conklin
    Article
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Inserts
    dCas9-KRAB-P2A-mCherry
    rtTA
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG and TRE3G
    Available Since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73497-plasmid-map-sequence-id-247304

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Showing: 121 - 140 of 172500 results