162,717 results

1. pCS2+HyPer7-NES

   Plasmid

   #136467

   Purpose

   Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   Nuclear export signal from the HIV Rev protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

   Plasmid

   #103865

   Purpose

   Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Contains the H133A and H1058A mutations which ina…

   Promoter

   EF1a

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-iCre

   Plasmid

   #89573

   Purpose

   Expresses iCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iCre
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. attB-puro donor.dna

   Plasmid

   #181923

   Purpose

   attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   attB-EGFP-EF1α-PuroR-T2A-TagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVpuro-CMV-N-mCherry

   Plasmid

   #123221

   Purpose

   N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. OpenCRISPR-1 sgRNA-12nt stem

   Plasmid

   #221567

   Purpose

   Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 12nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-GFP-Kir2.1

   Plasmid

   #107184

   Purpose

   mammalian expression of Kir2.1

   Depositor

   Bikash Pattnaik

   Insert

   KCNJ2 (KCNJ2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.2 TDP-43 YFP

    Plasmid

    #84911

    Purpose

    expression of TDP-43 YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-TurboID

    Plasmid

    #124646

    Purpose

    Expresses Flag-TurboID fusion for proximity labeling

    Depositor

    FENG-QIAN Li, Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYL192 (TRV1)

    Plasmid

    #148968

    Purpose

    Tobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Liu et al Plant J. 2002 May;30(4):415-29. doi: 10.

    Insert

    TRV RNA1
    

    Use

    Plant vector with trv rna1; this vector used with…

    Tags

    Expression

    Mutation

    P697S mutation in putative replicase. S239G and S…

    Promoter

    Available since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. IFN-Beta_pGL3

    Plasmid

    #102597

    Purpose

    IFN-beta promoter driving luciferase. Use in IFN reporter assay.

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    Human IFN-Beta promoter (IFNB1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-FUSN-mCh-Cry2WT

    Plasmid

    #101223

    Purpose

    FUS(1-214) fused to mCh-Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. tetO-SOX9

    Plasmid

    #170703

    Purpose

    doxycycline-inducible overexpression of SOX9

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-EGFP-C1-HsXRN1_K

    Plasmid

    #146784

    Purpose

    Mammalian Expression of HsXRN1

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    HsXRN1 (XRN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge