Purpose(Empty Backbone) 2nd generation lentiviral transfer plasmid
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12257||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
|Cloning Grade DNA||12257-DNA.cg||2 µg of cloning grade DNA in Tris buffer||1||$105|
This material is available to academics and nonprofits only.
- Backbone size (bp) 10510
Vector typeMammalian Expression, Lentiviral, Cre/Lox
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth instructionsUse Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
Copy numberHigh Copy
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site MCS - see map (not destroyed)
- 3′ cloning site MCS - see map (not destroyed)
- 5′ sequencing primer See map (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pWPT or pWPXL can be used for constitutive transgene expression.
pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter
The loxP site in the 3'LTR is duplicated to the 5'LTR during reverse transcription in the target cells. This allows for removal (if necessary) of an integrated provirus by Cre.
Unique restriction sites at key positions will allow you to change promoter and transgene. PmeI is a popular site for cloning. You can also use PacI and SwaI.
Please note that ClaI in this vector is blocked by Dam methylation. This plasmid needs to be grown in a Dam- bacteria strain if you wish to use ClaI for cloning.
Packaging plasmids for Trono lab lentiviral vectors are also available at Addgene http://www.addgene.org/rnaitools
Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.
Information for Cloning Grade DNA (Catalog # 12257-DNA.cg) ( Back to top )
Addgene no longer distributes this item. Contact [email protected] for more information.
2 µg of cloning grade DNA in Tris buffer
Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
- Amount 2 µg
- Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
- Pricing $105 USD
- Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).
Addgene has verified this plasmid using Next Generation Sequencing. Results are available here
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pWPXL was a gift from Didier Trono (Addgene plasmid # 12257 ; http://n2t.net/addgene:12257 ; RRID:Addgene_12257)