Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pEVOL-pAzF Citations (41)

Originally described in: Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli.
Chin JW, Santoro SW, Martin AB, King DS, Wang L, Schultz PG J Am Chem Soc. 2002 Aug 7;124(31):9026-7.
PubMed Journal

Articles Citing pEVOL-pAzF

Articles
Site-specific albumination of a therapeutic protein with multi-subunit to prolong activity in vivo. Lim SI, Hahn YS, Kwon I. J Control Release. 2015 Jun 10;207:93-100. doi: 10.1016/j.jconrel.2015.04.004. Epub 2015 Apr 7. PubMed
Evaluating Sense Codon Reassignment with a Simple Fluorescence Screen. Biddle W, Schmitt MA, Fisk JD. Biochemistry. 2015 Dec 22;54(50):7355-64. doi: 10.1021/acs.biochem.5b00870. Epub 2015 Dec 5. PubMed
Binding of the Covalent Flavin Assembly Factor to the Flavoprotein Subunit of Complex II. Maklashina E, Rajagukguk S, Starbird CA, McDonald WH, Koganitsky A, Eisenbach M, Iverson TM, Cecchini G. J Biol Chem. 2015 Dec 7. pii: jbc.M115.690396. PubMed
Site-Specific Albumination as an Alternative to PEGylation for the Enhanced Serum Half-Life in Vivo. Yang B, Lim SI, Kim JC, Tae G, Kwon I. Biomacromolecules. 2016 May 9;17(5):1811-7. doi: 10.1021/acs.biomac.6b00238. Epub 2016 Apr 15. PubMed
Site-Specific Labeling of Protein Kinase CK2: Combining Surface Display and Click Chemistry for Drug Discovery Applications. Nienberg C, Retterath A, Becher KS, Saenger T, Mootz HD, Jose J. Pharmaceuticals (Basel). 2016 Jun 27;9(3). pii: E36. doi: 10.3390/ph9030036. PubMed
Site-specific fluorescent labeling to visualize membrane translocation of a myristoyl switch protein. Yang ST, Lim SI, Kiessling V, Kwon I, Tamm LK. Sci Rep. 2016 Sep 8;6:32866. doi: 10.1038/srep32866. PubMed
In vitro suppression of two different stop codons. Ozer E, Chemla Y, Schlesinger O, Aviram HY, Riven I, Haran G, Alfonta L. Biotechnol Bioeng. 2016 Nov 24. doi: 10.1002/bit.26226. PubMed
Controlled Orientation of Active Sites in a Nanostructured Multienzyme Complex. Lim SI, Yang B, Jung Y, Cha J, Cho J, Choi ES, Kim YH, Kwon I. Sci Rep. 2016 Dec 22;6:39587. doi: 10.1038/srep39587. PubMed
Identification of a Potent Allosteric Inhibitor of Human Protein Kinase CK2 by Bacterial Surface Display Library Screening. Nienberg C, Garmann C, Gratz A, Bollacke A, Gotz C, Jose J. Pharmaceuticals (Basel). 2017 Jan 5;10(1). pii: E6. doi: 10.3390/ph10010006. PubMed
Semisynthetic Bioluminescent Sensor Proteins for Direct Detection of Antibodies and Small Molecules in Solution. Arts R, Ludwig SKJ, van Gerven BCB, Estirado EM, Milroy LG, Merkx M. ACS Sens. 2017 Nov 22;2(11):1730-1736. doi: 10.1021/acssensors.7b00695. Epub 2017 Nov 2. PubMed
The atlastin membrane anchor forms an intramembrane hairpin that does not span the phospholipid bilayer. Betancourt-Solis MA, Desai T, McNew JA. J Biol Chem. 2018 Nov 30;293(48):18514-18524. doi: 10.1074/jbc.RA118.003812. Epub 2018 Oct 4. PubMed
Chemically monoubiquitinated PEX5 binds to the components of the peroxisomal docking and export machinery. Hagmann V, Sommer S, Fabian P, Bierlmeier J, van Treel N, Mootz HD, Schwarzer D, Azevedo JE, Dodt G. Sci Rep. 2018 Oct 30;8(1):16014. doi: 10.1038/s41598-018-34200-5. PubMed
Conformation-specific detection of calmodulin binding using the unnatural amino acid p-azido-phenylalanine (AzF) as an IR-sensor. Creon A, Josts I, Niebling S, Huse N, Tidow H. Struct Dyn. 2018 Nov 7;5(6):064701. doi: 10.1063/1.5053466. eCollection 2018 Nov. PubMed
Efficient Incorporation of Unnatural Amino Acids into Proteins with a Robust Cell-Free System. Gao W, Bu N, Lu Y. Methods Protoc. 2019 Feb 12;2(1). pii: mps2010016. doi: 10.3390/mps2010016. PubMed
Engineered ribosomes with tethered subunits for expanding biological function. Carlson ED, d'Aquino AE, Kim DS, Fulk EM, Hoang K, Szal T, Mankin AS, Jewett MC. Nat Commun. 2019 Sep 2;10(1):3920. doi: 10.1038/s41467-019-11427-y. PubMed
Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modules. Rimbault C, Maruthi K, Breillat C, Genuer C, Crespillo S, Puente-Munoz V, Chamma I, Gauthereau I, Antoine S, Thibaut C, Tai FWJ, Dartigues B, Grillo-Bosch D, Claverol S, Poujol C, Choquet D, Mackereth CD, Sainlos M. Nat Commun. 2019 Oct 4;10(1):4521. doi: 10.1038/s41467-019-12528-4. PubMed

Associated Plasmids

RNA binding activates RIG-I by releasing an autorepressed signaling domain. Dickey TH, Song B, Pyle AM. Sci Adv. 2019 Oct 2;5(10):eaax3641. doi: 10.1126/sciadv.aax3641. eCollection 2019 Oct. PubMed
Interactions between PHD3-Bromo of MLL1 and H3K4me3 Revealed by Single-Molecule Magnetic Tweezers in a Parallel DNA Circuit. Ma X, Zhu M, Liu J, Li X, Qu L, Liang L, Huang W, Wang J, Li N, Chen JH, Zhang W, Yu Z. Bioconjug Chem. 2019 Dec 18;30(12):2998-3006. doi: 10.1021/acs.bioconjchem.9b00665. Epub 2019 Nov 15. PubMed
Single-molecule imaging of HIV-1 envelope glycoprotein dynamics and Gag lattice association exposes determinants responsible for virus incorporation. Pezeshkian N, Groves NS, van Engelenburg SB. Proc Natl Acad Sci U S A. 2019 Dec 10;116(50):25269-25277. doi: 10.1073/pnas.1910008116. Epub 2019 Nov 22. PubMed
Proximity-induced caspase-9 activation on a DNA origami-based synthetic apoptosome. Rosier BJHM, Markvoort AJ, Gumi Audenis B, Roodhuizen JAL, den Hamer A, Brunsveld L, de Greef TFA. Nat Catal. 2020 Mar;3(3):295-306. doi: 10.1038/s41929-019-0403-7. Epub 2020 Jan 6. PubMed
Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging. Hebbrecht T, Liu J, Zwaenepoel O, Boddin G, Van Leene C, Decoene K, Madder A, Braeckmans K, Gettemans J. N Biotechnol. 2020 Nov 25;59:33-43. doi: 10.1016/j.nbt.2020.05.004. Epub 2020 Jul 10. PubMed
Phosphoregulation of tropomyosin-actin interaction revealed using a genetic code expansion strategy. Palani S, Koester D, Balasubramanian MK. Wellcome Open Res. 2020 Jul 7;5:161. doi: 10.12688/wellcomeopenres.16082.1. eCollection 2020. PubMed
Nanobody-displaying porous silicon nanoparticles for the co-delivery of siRNA and doxorubicin. Tieu T, Wojnilowicz M, Huda P, Thurecht KJ, Thissen H, Voelcker NH, Cifuentes-Rius A. Biomater Sci. 2021 Jan 5;9(1):133-147. doi: 10.1039/d0bm01335h. PubMed
A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly. Groves NS, Bruns MM, van Engelenburg SB. Pathogens. 2020 Nov 21;9(11). pii: pathogens9110972. doi: 10.3390/pathogens9110972. PubMed
Site-by-site tracking of signal transduction in an azidophenylalanine-labeled bacteriophytochrome with step-scan FTIR spectroscopy. Kurttila M, Stucki-Buchli B, Rumfeldt J, Schroeder L, Hakkanen H, Liukkonen A, Takala H, Kottke T, Ihalainen JA. Phys Chem Chem Phys. 2021 Mar 11;23(9):5615-5628. doi: 10.1039/d0cp06553f. PubMed
Decoupling the bridge helix of Cas12a results in a reduced trimming activity, increased mismatch sensitivity and impaired conformational transitions. Worle E, Jakob L, Schmidbauer A, Zinner G, Grohmann D. Nucleic Acids Res. 2021 May 21;49(9):5278-5293. doi: 10.1093/nar/gkab286. PubMed
Genetic code expansion enables visualization of Salmonella type three secretion system components and secreted effectors. Singh MK, Zangoui P, Yamanaka Y, Kenney LJ. Elife. 2021 Jun 1;10. pii: 67789. doi: 10.7554/eLife.67789. PubMed
Probing Bioelectronic Connections Using Streptavidin Molecules with Modified Valency. Zhang B, Ryan E, Wang X, Lindsay S. J Am Chem Soc. 2021 Sep 22;143(37):15139-15144. doi: 10.1021/jacs.1c05569. Epub 2021 Sep 9. PubMed
Single-molecule conformational dynamics of a transcription factor reveals a continuum of binding modes controlling association and dissociation. Chen W, Lu W, Wolynes PG, Komives EA. Nucleic Acids Res. 2021 Nov 8;49(19):11211-11223. doi: 10.1093/nar/gkab874. PubMed
Visualizing an Allosteric Intermediate Using CuAAC Stabilization of an NMR Mixed Labeled Dimer. Sapienza PJ, Currie MM, Lancaster NM, Li K, Aube J, Goldfarb D, Cloer EW, Major MB, Lee AL. ACS Chem Biol. 2021 Dec 17;16(12):2766-2775. doi: 10.1021/acschembio.1c00617. Epub 2021 Nov 16. PubMed
A simple and general approach to generate photoactivatable DNA processing enzymes. Kesici MZ, Tinnefeld P, Vera AM. Nucleic Acids Res. 2022 Apr 8;50(6):e31. doi: 10.1093/nar/gkab1212. PubMed
Population Dynamics of Stretching Excitations of p-Azido-phenylalanine Incorporated in Calmodulin-Peptide Complexes. Ruppert M, Creon A, Tidow H, Huse N. J Phys Chem B. 2022 Jan 20;126(2):368-375. doi: 10.1021/acs.jpcb.1c06775. Epub 2022 Jan 6. PubMed
Mutations in respiratory complex I promote antibiotic persistence through alterations in intracellular acidity and protein synthesis. Van den Bergh B, Schramke H, Michiels JE, Kimkes TEP, Radzikowski JL, Schimpf J, Vedelaar SR, Burschel S, Dewachter L, Loncar N, Schmidt A, Meijer T, Fauvart M, Friedrich T, Michiels J, Heinemann M. Nat Commun. 2022 Jan 27;13(1):546. doi: 10.1038/s41467-022-28141-x. PubMed
ATP-responsive biomolecular condensates tune bacterial kinase signaling. Saurabh S, Chong TN, Bayas C, Dahlberg PD, Cartwright HN, Moerner WE, Shapiro L. Sci Adv. 2022 Feb 18;8(7):eabm6570. doi: 10.1126/sciadv.abm6570. Epub 2022 Feb 16. PubMed
Overexpression of recombinant proteins containing non-canonical amino acids in Vibrio natriegens: p-azido-L-phenylalanine as coupling site for (19)F-tags. Stadler KA, Becker W, Darnhofer B, Birner-Gruenberger R, Zangger K. Amino Acids. 2022 Jul;54(7):1041-1053. doi: 10.1007/s00726-022-03148-2. Epub 2022 Apr 13. PubMed
Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free Zn(2). Michielsen CMS, van Aalen EA, Merkx M. ACS Chem Biol. 2022 Jun 17;17(6):1567-1576. doi: 10.1021/acschembio.2c00227. Epub 2022 May 25. PubMed

Associated Plasmids

Efficient incorporation of p-azido-l-phenylalanine into the protein using organic solvents. Ma X, Wei B, Wang E. Protein Expr Purif. 2022 Dec;200:106158. doi: 10.1016/j.pep.2022.106158. Epub 2022 Aug 22. PubMed
Noncanonical amino acids as doubly bio-orthogonal handles for one-pot preparation of protein multiconjugates. Wang Y, Zhang J, Han B, Tan L, Cai W, Li Y, Su Y, Yu Y, Wang X, Duan X, Wang H, Shi X, Wang J, Yang X, Liu T. Nat Commun. 2023 Feb 21;14(1):974. doi: 10.1038/s41467-023-36658-y. PubMed
Site-Specific Modification of Virus-Like Particles for Exogenous Tumor Antigen Display and Minimizing Preexisting Immunity. Cheng K, Ma N, Liang J, Ma X, Feng Q, Liu G, Xu C, Tang M, Zhang L, Gao X, Xu J, Wang C, Zhu F, Wang X, Li X, Zhao X, Nie G. Small. 2023 Jun;19(23):e2300125. doi: 10.1002/smll.202300125. Epub 2023 Mar 6. PubMed
Genetically Encoded Noncanonical Amino Acids in Proteins to Investigate Lysine Benzoylation. Guo AD, Chen XH. Methods Mol Biol. 2023;2676:131-146. doi: 10.1007/978-1-0716-3251-2_9. PubMed
Design of Polarity-Dependent Immunosensors Based on the Structural Analysis of Engineered Antibodies. Islam J, Conroy P, Fercher C, Kim M, Yaari Z, Jones M, Bell TDM, Caradoc-Davies T, Law R, Whisstock J, Heller D, Mahler S, Corrie S. ACS Chem Biol. 2023 Aug 18;18(8):1863-1871. doi: 10.1021/acschembio.3c00303. Epub 2023 Jul 13. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.