We narrowed to 221 results for: nano bret

1. pcDNA3.1(+)Gαi1-91-NLuc

   Plasmid

   #207148

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI1 (GNAI1 Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)K

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)Gαi3-91-NLuc

   Plasmid

   #207150

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI3 (GNAI3 Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)K

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)Gαi2-91-NLuc

   Plasmid

   #207149

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI2 (GNAI2 Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)Q

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)Gαq-124-NLuc

   Plasmid

   #207153

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAQ (GNAQ Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(124)E

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)Gα13-125-NLuc

   Plasmid

   #207155

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA13 (GNA13 Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(125)D

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)Gα12-133-NLuc

   Plasmid

   #207156

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNA12 (GNA12 Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position A(133)F

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)GαsL-260-NLuc

   Plasmid

   #207158

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(260)N

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)GαsS-246-Nluc

   Plasmid

   #207157

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAS (GNAS Human)
   

   Expression

   Mammalian

   Mutation

   NLuc inserted at position D(246)N

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)Gγ1-LSS-mKATE2(N)

   Plasmid

   #207159

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG1 tagged with LSS-mKATE2 to be paired with one of the GNA

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNG1
   

   Expression

   Mammalian

   Mutation

   N-terminally tagged with LSS-mKATE2

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b(+)_S-Luc_CLIP

    Plasmid

    #113923

    Purpose

    Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    S-Luc_CLIP
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

    Plasmid

    #207160

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNG2 (GNG2 Human)
    

    Expression

    Mammalian

    Mutation

    N-terminally tagged with LSS-mKATE2

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. nLuc-PTHR1

    Plasmid

    #162577

    Purpose

    Expresses N-terminally NanoLuc-Tagged PTHR1 in mammalian cells

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    Parathyroid Hormone Receptor 1 (PTH1R Human)
    

    Tags

    Nanoluc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. nLuc-ΔECD-PTHR1

    Plasmid

    #190011

    Purpose

    Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    nLuc-ΔECD-PTHR1 (PTH1R Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nluc-YAP1_TEAD4-HaloTag Bidirectional(Balanced) Vector

    Plasmid

    #237003

    Purpose

    Express NLuc-YAP1_TEAD4-HaloTag(R) Fusion Proteins in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    YAP1 (YAP1 Human)
    TEAD4 (TEAD4 Human)
    

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pNLF1W

    Plasmid

    #136404

    Purpose

    Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. G12-CASE

    Plasmid

    #190714

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.

    Inserts

    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    

    Use

    Luciferase

    Tags

    Nluc is inserted at A133/F134 within GNA12 and cp…

    Expression

    Mammalian

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. NbAlfa-GFP2

    Plasmid

    #201485

    Purpose

    BRET2 detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbAlfa-GFP2
    

    Tags

    GFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NbV5-GFP2

    Plasmid

    #201477

    Purpose

    BRET2 detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    NbV5-GFP2
    

    Tags

    GFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP2-NbALFA

    Plasmid

    #201486

    Purpose

    BRET2 detection of ALFA-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    GFP2-NbALFA
    

    Tags

    GFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP2-NbV5

    Plasmid

    #201478

    Purpose

    BRET2 detection of V5-tagged protein

    Depositor

    Patrick Giguère

    Article

    Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

    Insert

    GFP2-NbV5
    

    Tags

    GFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge