We narrowed to 7,332 results for: 11

1. pGEX6P1-hPREP

   Plasmid

   #179387

   Purpose

   Express human prolyl oligopeptidase (tagged) in bacterial cells

   Depositor

   Christian Gruber

   Article

   Retzl et al J Nat Prod. 2020 Nov 25;83(11):3305-3314

   Insert

   human prolyl oligopeptidase (PREP Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   TAC

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIntegrase_7

   Plasmid

   #60578

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_7
   

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-nucGFP11-3xControlgRNA

   Plasmid

   #224568

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFosill-2

   Plasmid

   #89696

   Purpose

   to make Fosmids that can be converted into Illumina sequencable libraries

   Depositor

   Andreas Gnirke

   Article

   Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-nDsRedFL-intron

   Plasmid

   #177804

   Purpose

   An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

   Depositor

   Rikako Sanuki

   Article

   Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

   Insert

   intron
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Pdgfra-T2A-H2B-eGFP donor

   Plasmid

   #113120

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGinB-8X(GGS)-dCas9-FLAG-NLS

   Plasmid

   #81205

   Purpose

   CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

   Depositor

   David Liu

   Article

   Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

   Insert

   pGinB-8X(GGS)-dCas9-FLAG-NLS
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCFJ906

   Plasmid

   #44472

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, unc-119, [4-3] Gateway
   

   Use

   Gateway destination vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMRX-INU-TEX264 LIR4A-FLAG

   Plasmid

   #128259

   Purpose

   Expresses TEX264 LIR4A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   TEX264 LIR4A (TEX264 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed FEEL (aa 273-276) to alanines

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP2
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pFosill-4

    Plasmid

    #89698

    Purpose

    to make Fosmids that can be converted into Illumina sequencable libraries

    Depositor

    Andreas Gnirke

    Article

    Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RhoC FLARE.sc mCer, mVenus - CA

    Plasmid

    #65424

    Purpose

    RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus, Q63L in RhoC

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

13. RhoC FLARE.sc mCer, mVenus - DN

    Plasmid

    #65423

    Purpose

    RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus, T19N in RhoC

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRK5 mFz8CRD-IgG

    Plasmid

    #16689

    Depositor

    Xi He

    Article

    Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

    Insert

    Frizzled-8 (Fzd8 Mouse)
    

    Tags

    IgG

    Expression

    Mammalian

    Mutation

    CRD domain of mFz8

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

15. GFP-V96 ELP

    Plasmid

    #67853

    Purpose

    GFP Fluorescent Elastin-like Polypeptide(VPGVG) with 96 repeats containing Valine guest residues

    Depositor

    Andrew MacKay

    Article

    Pastuszka et al Biomacromolecules. 2012 Nov 12;13(11):34

    Insert

    GFP fused to ELP (VPGVG) repeated 96 times
    

    Tags

    GFP fused to N-terminal of ELP

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

    Plasmid

    #183069

    Purpose

    Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c_4
    

    Use

    CRISPR

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    Wild-type

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIntegrase_5

    Plasmid

    #60577

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_5
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFBD-ESCRT 0

    Plasmid

    #21499

    Depositor

    James Hurley

    Article

    Ren et al Structure. 2009 Mar 11. 17(3):406-16.

    Insert

    pFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Mouse, Human)
    

    Tags

    GST and His-MBP

    Expression

    Insect

    Mutation

    none

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

19. EYFP-Clathrin

    Plasmid

    #20921

    Depositor

    Xiaowei Zhuang

    Article

    Rust et al Nat Struct Mol Biol. 2004 Jun . 11(6):56

    Insert

    Clathrin, light polypeptide (Lca) (Clta Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pPLtetO-ClpX

    Plasmid

    #89657

    Purpose

    Plasmid for titrating synthesis of ClpX with doxycycline

    Depositor

    Uwe Sauer

    Article

    Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

    Insert

    clpX
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge