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Two new CRISPR tools from the Abudayyeh-Gootenberg lab are now available at Addgene: Cas7/11 and PASTE.

An overview of split fluorescent proteins and a highlight of some of the most popular and newest ones available on Addgene.

Senior graduate student researchers Niroshi Senaratne and Ben Vincent provide concrete advice on how to pick a lab, a mentor, and a research project.

FlipGFP is a genetically encoded caspase reporter that contains a flipped beta strand of GFP. There is no fluorescence unless the caspase cuts the cleavage site between the strands.

Comparing and contrasting Cas9 to the other genome editing-capable Cas family proteins.

How to develop a supportive grad student community .The basics - be proactive, choose a good mentor, and start thinking about your career early.

Our depositors don't just deposit great plasmids - they also do some incredible science! Check out some incredible depositor research stories from 2022.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

In this podcast, learn about Kingdom Kwapata, a Malawian researcher currently developing transgenic crops and new means to produce biofuels.

Ghanaian researcher, Kwabena Duedu discusses his work in public health, synthetic biology, and microbiology.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Check out how you can use digital PCR to validate your CRISPR genome edits.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Learn what a high fidelity DNA ligase is and when you might need one.

Learn how a new episomal plasmid-based method can be used to efficiently reprogram blood cells to induced pluripotent stem cells

Use the mRNA expression FlowCode plasmids for barcoded expression of mRNAs of interest, for use in large-scale multiplexed in vivo mRNA expression screens.

Find plasmids encoding fluorescent biosensors to measure biomolecules or genes via FRET or other assays.

From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently.

Exciting new plasmids, viruses, and antibodies in the Addgene collection!

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.