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We narrowed to 123 results for: 11

Showing: 1 - 20 of 123 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...CRISPR effector protein they named Cas7-11. Testing showed that Cas7-11 cut RNA with unprecedented specificity...luciferase RNA knockdown assays using Cas7-11   Use The Cas7-11 targeting system contains two pieces: ...both of which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest ...looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth inhibition when...negative effects in RNA targeting experiments. If Cas7-11 lacked this toxicity, it would represent a major ...  The team originally suspected that this new Cas7-11 might be phylogenetically classified as the more ...observations, Koonin and Makarova found that Cas7-11 appeared to have derived from the more complex type...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...are split between strand 10 and 11 to yield large (1-10) and small (11) fragments. In several cases an... FP(11) fragments, even from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). ... Figure 1: Two proteins are fused to the FP(11) and FP(1-10) fragments. When Protein A and B interact...EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...-folder GFP, our most-requested GFP(1-10) and GFP(11) Versatile protein tagging in cells with split fluorescent...Brian McNaughton mNeonGreen2 mNG2(1-10) and mNG2(11) Improved split fluorescent proteins for endogenous...

  3. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...doing rotations? 8:01 - 11:25 : How did you decide what lab to join? 11:25 - 11:50 : What is the number...rotation students should ask senior graduate students? 11:51 - 13:28 : How do you define what a good mentor...What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental ...

  4. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...FlipGFP, sfCherry was split into  β1-9 and β10-11, where β10-11 was forced into a parallel conformation by...different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a single β-strand of GFP, hence the ...et al., 2019). How does FlipGFP work? GFP contains 11 β-strands and one central ɑ-helix that can be split... ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues...was dependent on protease activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands...thus can no longer fit with β1-9. They connected β10-11 with two linkers (E5 and K5) that dimerize together...

  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...Best for: targeting single stranded RNA.   Cas7-11 Cas7-11 is a naturally occurring RNase composed of the...between Cas7-11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display...the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts ssRNA and is capable of pre-crRNA self-processing... of collateral cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/s41586-021-...

  6. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    Published
    Oct. 6, 2016, 2:30 p.m.
    ...give their insights as to what causes this dip. 11:11 - 15:37 : How have you changed throughout graduate....com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate school and what makes...

  7. 8 Great Depositor Research Stories

    Type
    Blog Post
    Published
    Dec. 15, 2022, 2:15 p.m.
    ..., a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh and Jonathan Gootenberg...worked with us to share their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   Figure 1: Single-phage...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288. PubMed ...sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355. PubMed ... next-generation antimicrobials.” Genome Biol. 15(11):516. (2014). PubMed: PMID 25417800. PubMed Central...

  10. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type
    Blog Post
    Published
    Dec. 20, 2016, 3:30 p.m.
    ...listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction - Ghanaian scientist's molecular biology...the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at...

  11. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    .... J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904.   ...new variation on their previous sensor STReTCh, the 11-amino acid BiT fragment of luciferase is embedded... Society. Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors...

  12. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ... iPS cells without antibiotic selection. Nat Meth 11, 291–293 (2014). PubMed PMID: 24509632. PubMed Central...Specific Screening of Genome Edited Cells. PLoS ONE 11, e0153901–17 (2016). PubMed PMID: 27089539. PubMed...-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, ...

  14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...substrates containing gaps or DNA base pair mismatches (8-11). For some applications, there cannot be any end-... near the ligation junction with high efficiency (11, 21). Ligases are thought to interrogate dsDNA for...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al. "Cloning...PubMed PMID: 3684572. PubMed Central PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of...

  16. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...Additional human IgG1s are also available on demand. The 11 IPI synthetic rAbs, developed using yeast display... integrins, the RGD-binding integrins. Six of the 11 IPI antibodies contain RGD-mimetic sequences that...binding specificity, kinetics, and affinity of these 11 newly discovered synthetic rAbs. We then used these...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm...via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub...

  19. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...effector was developed, called Cas7-11. Despite being in Class 1, Cas7-11 is a single protein effector and...CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Makarova...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597(7878), 720–725. https://doi.org/10.1038...

  20. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...keratinocytes. Nat Biotechnol, 2008. 26(11): p. 1276-84. PubMed PMID: 18931654. 11. Loh, Y.H., et al., Reprogramming...generation of human cardiomyocytes. Nat Methods, 2014. 11(8): p. 855-60. PubMed PMID: 24930130. PubMed Central...induced pluripotent stem cells. Stem Cell Rev, 2015. 11(2): p. 242-53. PubMed PMID: 25578634. PubMed Central...
Showing: 1 - 20 of 123 results