...CRISPR effector protein they named Cas7-11. Testing showed that Cas7-11 cut RNA with unprecedented specificity...luciferase RNA knockdown assays using Cas7-11   Use The Cas7-11 targeting system contains two pieces: ...both of which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest ...looked at the transformed E. coli expressing Cas7-11, they found no evidence of growth inhibition when...negative effects in RNA targeting experiments. If Cas7-11 lacked this toxicity, it would represent a major ...  The team originally suspected that this new Cas7-11 might be phylogenetically classified as the more ...observations, Koonin and Makarova found that Cas7-11 appeared to have derived from the more complex type...

...are split between strand 10 and 11 to yield large (1-10) and small (11) fragments. In several cases an... FP(11) fragments, even from a different base FP (for example, EBFP2(1-10) can be used with GFP(11)). ... Figure 1: Two proteins are fused to the FP(11) and FP(1-10) fragments. When Protein A and B interact...EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...Kamiyama Cerulean Cerulean(1-10) for use with GFP(11) Multiplexed labeling of cellular proteins with split...-folder GFP, our most-requested GFP(1-10) and GFP(11) Versatile protein tagging in cells with split fluorescent...Brian McNaughton mNeonGreen2 mNG2(1-10) and mNG2(11) Improved split fluorescent proteins for endogenous...

...doing rotations? 8:01 - 11:25 : How did you decide what lab to join? 11:25 - 11:50 : What is the number...rotation students should ask senior graduate students? 11:51 - 13:28 : How do you define what a good mentor...What's been your biggest experimental failure? 26:11 - 31: 32 : What's been your biggest experimental ...

...FlipGFP, sfCherry was split into  β1-9 and β10-11, where β10-11 was forced into a parallel conformation by...different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a single β-strand of GFP, hence the ...et al., 2019). How does FlipGFP work? GFP contains 11 β-strands and one central ɑ-helix that can be split... ɑ-helix, (2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues...was dependent on protease activation. Normally, β10-11 are antiparallel. To prevent binding, the β strands...thus can no longer fit with β1-9. They connected β10-11 with two linkers (E5 and K5) that dimerize together...

...Best for: targeting single stranded RNA.   Cas7-11 Cas7-11 is a naturally occurring RNase composed of the...between Cas7-11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity. Cas7-11 does not display...the fusion of several Cas7 and Cas11 domains. Cas7-11 cuts ssRNA and is capable of pre-crRNA self-processing... of collateral cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597, 720-725 (2021). 10.1038/s41586-021-...

...give their insights as to what causes this dip. 11:11 - 15:37 : How have you changed throughout graduate....com/watch?v=lQGAsgjjBDI&feature=youtu.be. 8:17 - 11:10 : The 3rd year of graduate school and what makes...

... the proportion of virulent S. aureus from 50% to 11% in just 24 hours. Improving CRISPR delivery and...efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed: PMID 252409288. PubMed ...sequence-specific antimicrobials.” Nature Biotechnol. 32(11): 1146-50. (2014). PubMed: PMID 25282355. PubMed ... next-generation antimicrobials.” Genome Biol. 15(11):516. (2014). PubMed: PMID 25417800. PubMed Central...

..., a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh and Jonathan Gootenberg...worked with us to share their new CRISPR tools, Cas7-11, a low-toxicity RNA editing tool, and PASTE, a drag-and-drop...

...10:30-11:41 : Kingdom on the difficulties surrouding and need for biotech research in Malawi 11:41-14:...

...listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 : Introduction - Ghanaian scientist's molecular biology...the Addgene, Seeding Labs plasmid grant. 7:12 - 7:11 : Kwabena's work in Christopher E. French's Lab at...

... iPS cells without antibiotic selection. Nat Meth 11, 291–293 (2014). PubMed PMID: 24509632. PubMed Central...Specific Screening of Genome Edited Cells. PLoS ONE 11, e0153901–17 (2016). PubMed PMID: 27089539. PubMed...-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed PMID: 26914317. 4. Mock, ...

...layers, form embryoid bodies, and form teratomas [11]. iPSCs also possess similar morphologies, growth...properties, and express stem cell markers similar to ESCs [11].  While iPSCs and ESCs have similar functional utility...2007. 318(5858): p. 1917-20. PubMed PMID: 18029452. 11. Medvedev, S.P., A.I. Shevchenko, and S.M. Zakian...

...substrates containing gaps or DNA base pair mismatches (8-11). For some applications, there cannot be any end-... near the ligation junction with high efficiency (11, 21). Ligases are thought to interrogate dsDNA for...thermostable DNA ligase-encoding gene." Gene 109.1 (1991): 1-11. PubMed PMID: 1756968. 7. Lauer, G., et al. "Cloning...PubMed PMID: 3684572. PubMed Central PMCID: PMC306403. 11. Wu, Dan Y., and R. Bruce Wallace. "Specificity of...

...generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying the enhancement...non-integrating episomal vectors. PLoS One, 2011. 6(11): p. e27956. PubMed PMID: 22132178. PubMed Central...PMID: 19668191. PubMed Central PMCID: PMC2917235. 11. Nakagawa, M., et al., Promotion of direct reprogramming...

... mRNA with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm...via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub...

...Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. PubMed. Find the plasmids...

...Additional human IgG1s are also available on demand. The 11 IPI synthetic rAbs, developed using yeast display... integrins, the RGD-binding integrins. Six of the 11 IPI antibodies contain RGD-mimetic sequences that...binding specificity, kinetics, and affinity of these 11 newly discovered synthetic rAbs. We then used these...

...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15731-w Wang... ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 . https://doi.org/10.1371/journal.pone.0154027...

...keratinocytes. Nat Biotechnol, 2008. 26(11): p. 1276-84. PubMed PMID: 18931654. 11. Loh, Y.H., et al., Reprogramming...generation of human cardiomyocytes. Nat Methods, 2014. 11(8): p. 855-60. PubMed PMID: 24930130. PubMed Central...induced pluripotent stem cells. Stem Cell Rev, 2015. 11(2): p. 242-53. PubMed PMID: 25578634. PubMed Central...

...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can...